PMID- 27020355
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20160330
LR  - 20160329
IS  - 0022-3646 (Print)
IS  - 0022-3646 (Linking)
VI  - 47
IP  - 6
DP  - 2011 Dec
TI  - EFFECT OF ELEVATED TEMPERATURE, DARKNESS, AND HYDROGEN PEROXIDE TREATMENT ON 
      OXIDATIVE STRESS AND CELL DEATH IN THE BLOOM-FORMING TOXIC CYANOBACTERIUM 
      MICROCYSTIS AERUGINOSA(1).
PG  - 1316-25
LID - 10.1111/j.1529-8817.2011.01074.x [doi]
AB  - This study assessed the implication of oxidative stress in the mortality of cells 
      of Microcystis aeruginosa Kutz. Cultures grown at 25 degrees C were exposed to 32 degrees C, 
      darkness, and hydrogen peroxide (0.5 mM) for 96 h. The cellular abundance, chl a 
      concentration and content, maximum photochemical efficiency of PSII (Fv /Fm 
      ratio), intracellular oxidative stress (determined with dihydrorhodamine 123 
      [DHR]), cell mortality (revealed by SYTOX-labeling of DNA), and activation of 
      caspase 3-like proteins were assessed every 24 h. The presence of DNA degradation 
      in cells of M. aeruginosa was also assessed using a terminal deoxynucletidyl 
      transferase-mediated dUTP nick end labeling (TUNEL) assay at 96 h. Transferring 
      cultures from 25 degrees C to 32 degrees C was generally beneficial to the cells. The cellular 
      abundance and chl a concentration increased, and the mortality remained low 
      (except for a transient burst at 72 h) as did the oxidative stress. In darkness, 
      cells did not divide, and the Fv /Fm continuously decreased with time. The slow 
      increase in intracellular oxidative stress coincided with the activation of 
      caspase 3-like proteins and a 15% and 17% increase in mortality and 
      TUNEL-positive cells, respectively. Exposure to hydrogen peroxide had the most 
      detrimental effect on cells as growth ceased and the Fv /Fm declined to near zero 
      in less than 24 h. The 2-fold increase in oxidative stress matched the activation 
      of caspase 3-like proteins and a 40% and 37% increase in mortality and 
      TUNEL-positive cells, respectively. These results demonstrate the implication of 
      oxidative stress in the stress response and mortality of M. aeruginosa.
CI  - (c) 2011 Phycological Society of America.
FAU - Bouchard, Josee N
AU  - Bouchard JN
AD  - University of Southampton, National Oceanography Centre, Waterfront Campus, 
      European Way, Southampton, UK, SO14 3ZH.
FAU - Purdie, Duncan A
AU  - Purdie DA
AD  - University of Southampton, National Oceanography Centre, Waterfront Campus, 
      European Way, Southampton, UK, SO14 3ZH.
LA  - eng
PT  - Journal Article
DEP - 20111104
PL  - United States
TA  - J Phycol
JT  - Journal of phycology
JID - 9882935
OTO - NOTNLM
OT  - DNA degradation
OT  - Microcystis aeruginosa
OT  - SYTOX-green
OT  - caspase 3-like activity
OT  - cell death
OT  - cyanobacteria
OT  - darkness
OT  - hydrogen peroxide
OT  - oxidative stress
OT  - temperature
OT  - terminal deoxynucletidyl transferase-mediated dUTP nick end labeling
EDAT- 2011/12/01 00:00
MHDA- 2011/12/01 00:01
CRDT- 2016/03/30 06:00
PHST- 2016/03/30 06:00 [entrez]
PHST- 2011/12/01 00:00 [pubmed]
PHST- 2011/12/01 00:01 [medline]
AID - 10.1111/j.1529-8817.2011.01074.x [doi]
PST - ppublish
SO  - J Phycol. 2011 Dec;47(6):1316-25. doi: 10.1111/j.1529-8817.2011.01074.x. Epub 
      2011 Nov 4.