PMID- 27020413
OWN - NLM
STAT- MEDLINE
DCOM- 20171117
LR  - 20201209
IS  - 1574-6968 (Electronic)
IS  - 0378-1097 (Linking)
VI  - 363
IP  - 9
DP  - 2016 May
TI  - Susceptibility of Klebsiella pneumoniae on coriander leaves to liquid- and 
      vapor-phase ethanol.
LID - fnw072 [pii]
LID - 10.1093/femsle/fnw072 [doi]
AB  - The bio-control of ethanol on Klebsiella pneumoniae on fresh coriander leaves for 
      significantly reducing consumer health risk was investigated. Washed and 
      sterilized leaves of coriander were inoculated with K. pneumoniae cultured in 
      Trypticase Soy broth. Susceptibility of the K. pneumoniae to liquid- and 
      evaporated vapor-phase ethanol (EVE) was then examined in vitro Complete 
      inhibition of K. pneumoniae was found with 18% (v/v) liquid ethanol. Exposure for 
      15 min to EVE (9.00 +/- 0.8 mmol L(-1)) completely destroyed K. pneumoniae (4.04 +/- 
      0.02 log CFU/ml) spread on Mueller Hilton agar at 30 +/- 2 degrees C. The effect of EVE 
      with and without evaporated water vapor (EWV) on the susceptibility of K. 
      pneumoniae on fresh coriander leaves was examined. While exposure to EVE affected 
      the survival of K. pneumoniae, the degree of reduction depended on both the 
      inoculation level and the EWV. Complete reduction of K. pneumoniae was achieved 
      for the low inoculation level by EVE alone (37 +/- 2% relative humidity; RH) but 
      susceptibility was reduced with EWV (high RH; 80 +/- 2%). Scanning electron 
      microscope (SEM) images of inoculated coriander leaves confirm the effects of EVE 
      in reducing levels of K. pneumoniae Exposure to EVE alone proved an effective 
      bio-control for K. pneumoniae on fresh coriander leaves.
CI  - (c) FEMS 2016. All rights reserved. For permissions, please e-mail: 
      journals.permissions@oup.com.
FAU - Krusong, Warawut
AU  - Krusong W
AD  - Division of Fermentation Technology, Faculty of Agro-Industry, King Mongkut's 
      Institute of Technology Ladkrabang, Chalong Krung Rd, Bangkok 10520, Thailand 
      warawut.kr@kmitl.ac.th.
FAU - Pornpukdeewatana, Soisuda
AU  - Pornpukdeewatana S
AD  - Division of Fermentation Technology, Faculty of Agro-Industry, King Mongkut's 
      Institute of Technology Ladkrabang, Chalong Krung Rd, Bangkok 10520, Thailand.
FAU - Teerarak, Montinee
AU  - Teerarak M
AD  - Department of Plant Production Technology, Faculty of Agricultural Technology, 
      King Mongkut's Institute of Technology Ladkrabang, Chalong Krung Rd, Bangkok 
      10520, Thailand.
LA  - eng
PT  - Journal Article
DEP - 20160327
PL  - England
TA  - FEMS Microbiol Lett
JT  - FEMS microbiology letters
JID - 7705721
RN  - 0 (Steam)
RN  - 3K9958V90M (Ethanol)
SB  - IM
MH  - Coriandrum/*microbiology
MH  - Ethanol/chemistry/*pharmacology
MH  - Food Microbiology/methods
MH  - Humidity
MH  - Klebsiella pneumoniae/*drug effects/growth & development
MH  - Plant Leaves/*microbiology
MH  - Steam
MH  - Volatilization
OTO - NOTNLM
OT  - Klebsiella pneumoniae
OT  - evaporated vapor-phase ethanol
OT  - evaporated water vapor
OT  - fresh coriander
OT  - relative humidity
EDAT- 2016/03/30 06:00
MHDA- 2017/11/29 06:00
CRDT- 2016/03/30 06:00
PHST- 2016/03/21 00:00 [accepted]
PHST- 2016/03/30 06:00 [entrez]
PHST- 2016/03/30 06:00 [pubmed]
PHST- 2017/11/29 06:00 [medline]
AID - fnw072 [pii]
AID - 10.1093/femsle/fnw072 [doi]
PST - ppublish
SO  - FEMS Microbiol Lett. 2016 May;363(9):fnw072. doi: 10.1093/femsle/fnw072. Epub 
      2016 Mar 27.