PMID- 27020634
OWN - NLM
STAT- MEDLINE
DCOM- 20170103
LR  - 20191113
IS  - 1499-3872 (Print)
VI  - 15
IP  - 2
DP  - 2016 Apr
TI  - Efficient generation of functional hepatocyte-like cells from mouse liver 
      progenitor cells via indirect co-culture with immortalized human hepatic stellate 
      cells.
PG  - 173-9
AB  - BACKGROUND: Differentiation of liver progenitor cells (LPCs) to functional 
      hepatocytes holds great potential to develop new strategies for hepatocyte 
      transplantation and the screening of drug-induced cytotoxicity. However, reports 
      on the efficient and convenient hepatic differentiation of LPCs to hepatocytes 
      are few. The present study aims to investigate the possibility of generating 
      functional hepatocytes from LPCs in an indirect co-culture system. METHODS: Mouse 
      LPCs were co-cultured in Transwell plates with an immortalized human hepatic 
      stellate cell line (HSC-Li) we previously established. The morphology, expression 
      of hepatic markers, and functions of mouse LPC-derived cells were monitored and 
      compared with those of conventionally cultured LPCs. RESULTS: Co-culturing with 
      HSC-Li cells induced differentiation of mouse LPCs into functional 
      hepatocyte-like cells. The differentiated cells were morphologically transformed 
      into hepatocyte-like cells 3 days after co-culture initiation. In addition, the 
      differentiated cells expressed liver-specific genes and possessed hepatic 
      functions, including glycogen storage, low-density lipoprotein uptake, albumin 
      secretion, urea synthesis, and cytochrome P450 1A2 enzymatic activity. 
      CONCLUSIONS: Our method, which employs indirect co-culture with HSC-Li cells, can 
      efficiently induce the differentiation of LPCs into functional hepatocytes. This 
      finding suggests that this co-culture system can be a useful method for the 
      efficient generation of functional hepatocytes from LPCs.
FAU - Pan, Xiao-Ping
AU  - Pan XP
AD  - Collaborative Innovation Center for Diagnosis and Treatment of Infectious 
      Diseases; State Key Laboratory for Diagnosis and Treatment of Infectious 
      Diseases, First Affiliated Hospital, Zhejiang University School of Medicine, 
      Hangzhou 310003, China. ljli@zju.edu.cn.
FAU - Wang, Yi-Ni
AU  - Wang YN
FAU - Yu, Xiao-Peng
AU  - Yu XP
FAU - Zhu, Chun-Xia
AU  - Zhu CX
FAU - Li, Jian-Zhou
AU  - Li JZ
FAU - Du, Wei-Bo
AU  - Du WB
FAU - Zhang, Yi-Min
AU  - Zhang YM
FAU - Cao, Hong-Cui
AU  - Cao HC
FAU - Zhang, Yan-Hong
AU  - Zhang YH
FAU - Zhu, Dan-Hua
AU  - Zhu DH
FAU - Yeoh, George C
AU  - Yeoh GC
FAU - Li, Lan-Juan
AU  - Li LJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Singapore
TA  - Hepatobiliary Pancreat Dis Int
JT  - Hepatobiliary & pancreatic diseases international : HBPD INT
JID - 101151457
RN  - 0 (Albumins)
RN  - 0 (Biomarkers)
RN  - 0 (Culture Media, Conditioned)
RN  - 0 (Lipoproteins, LDL)
RN  - 8W8T17847W (Urea)
RN  - 9005-79-2 (Glycogen)
RN  - EC 1.14.14.1 (Cytochrome P-450 CYP1A2)
SB  - IM
MH  - Albumins/metabolism
MH  - Animals
MH  - Biomarkers/metabolism
MH  - *Cell Differentiation
MH  - Cell Line
MH  - Cell Shape
MH  - Coculture Techniques
MH  - Culture Media, Conditioned/metabolism
MH  - Cytochrome P-450 CYP1A2/metabolism
MH  - Gene Expression Regulation
MH  - Glycogen/metabolism
MH  - Hepatic Stellate Cells/*metabolism
MH  - Hepatocytes/*metabolism
MH  - Humans
MH  - Lipoproteins, LDL/metabolism
MH  - Liver/cytology/*metabolism
MH  - Male
MH  - Mice, Inbred C57BL
MH  - *Paracrine Communication
MH  - Phenotype
MH  - Stem Cells/*metabolism
MH  - Time Factors
MH  - Urea/metabolism
EDAT- 2016/03/30 06:00
MHDA- 2017/01/04 06:00
CRDT- 2016/03/30 06:00
PHST- 2016/03/30 06:00 [entrez]
PHST- 2016/03/30 06:00 [pubmed]
PHST- 2017/01/04 06:00 [medline]
AID - S1499-3872(16)60074-7 [pii]
AID - 10.1016/s1499-3872(16)60074-7 [doi]
PST - ppublish
SO  - Hepatobiliary Pancreat Dis Int. 2016 Apr;15(2):173-9. doi: 
      10.1016/s1499-3872(16)60074-7.