PMID- 27020958
OWN - NLM
STAT- MEDLINE
DCOM- 20171107
LR  - 20220408
IS  - 1532-298X (Electronic)
IS  - 1040-4651 (Print)
IS  - 1040-4651 (Linking)
VI  - 28
IP  - 4
DP  - 2016 Apr
TI  - UV-B Perception and Acclimation in Chlamydomonas reinhardtii.
PG  - 966-83
LID - 10.1105/tpc.15.00287 [doi]
AB  - Plants perceive UV-B, an intrinsic component of sunlight, via a signaling pathway 
      that is mediated by the photoreceptor UV RESISTANCE LOCUS8 (UVR8) and induces 
      UV-B acclimation. To test whether similar UV-B perception mechanisms exist in the 
      evolutionarily distant green alga Chlamydomonas reinhardtii, we identified 
      Chlamydomonas orthologs of UVR8 and the key signaling factor CONSTITUTIVELY 
      PHOTOMORPHOGENIC1 (COP1). Cr-UVR8 shares sequence and structural similarity to 
      Arabidopsis thaliana UVR8, has conserved tryptophan residues for UV-B 
      photoreception, monomerizes upon UV-B exposure, and interacts with Cr-COP1 in a 
      UV-B-dependent manner. Moreover, Cr-UVR8 can interact with At-COP1 and complement 
      the Arabidopsis uvr8 mutant, demonstrating that it is a functional UV-B 
      photoreceptor. Chlamydomonas shows apparent UV-B acclimation in colony survival 
      and photosynthetic efficiency assays. UV-B exposure, at low levels that induce 
      acclimation, led to broad changes in the Chlamydomonas transcriptome, including 
      in genes related to photosynthesis. Impaired UV-B-induced activation in the 
      Cr-COP1 mutant hit1 indicates that UVR8-COP1 signaling induces transcriptome 
      changes in response to UV-B. Also, hit1 mutants are impaired in UV-B acclimation. 
      Chlamydomonas UV-B acclimation preserved the photosystem II core proteins D1 and 
      D2 under UV-B stress, which mitigated UV-B-induced photoinhibition. These 
      findings highlight the early evolution of UVR8 photoreceptor signaling in the 
      green lineage to induce UV-B acclimation and protection.
CI  - (c) 2016 American Society of Plant Biologists. All rights reserved.
FAU - Tilbrook, Kimberley
AU  - Tilbrook K
AUID- ORCID: 0000-0001-5394-1996
AD  - Department of Botany and Plant Biology, University of Geneva, Sciences III, 
      CH-1211 Geneva 4, Switzerland.
FAU - Dubois, Marine
AU  - Dubois M
AUID- ORCID: 0000-0003-3579-1829
AD  - Department of Botany and Plant Biology, University of Geneva, Sciences III, 
      CH-1211 Geneva 4, Switzerland.
FAU - Crocco, Carlos D
AU  - Crocco CD
AUID- ORCID: 0000-0002-6026-4003
AD  - Department of Botany and Plant Biology, University of Geneva, Sciences III, 
      CH-1211 Geneva 4, Switzerland.
FAU - Yin, Ruohe
AU  - Yin R
AUID- ORCID: 0000-0001-6782-4651
AD  - Department of Botany and Plant Biology, University of Geneva, Sciences III, 
      CH-1211 Geneva 4, Switzerland.
FAU - Chappuis, Richard
AU  - Chappuis R
AD  - Department of Botany and Plant Biology, University of Geneva, Sciences III, 
      CH-1211 Geneva 4, Switzerland.
FAU - Allorent, Guillaume
AU  - Allorent G
AD  - Department of Botany and Plant Biology, University of Geneva, Sciences III, 
      CH-1211 Geneva 4, Switzerland.
FAU - Schmid-Siegert, Emanuel
AU  - Schmid-Siegert E
AUID- ORCID: 0000-0003-1339-5120
AD  - SIB-Swiss Institute of Bioinformatics, University of Lausanne, CH-1015 Lausanne, 
      Switzerland.
FAU - Goldschmidt-Clermont, Michel
AU  - Goldschmidt-Clermont M
AUID- ORCID: 0000-0003-1224-5868
AD  - Department of Botany and Plant Biology, University of Geneva, Sciences III, 
      CH-1211 Geneva 4, Switzerland Institute of Genetics and Genomics of Geneva 
      (iGE3), University of Geneva, CH-1211 Geneva 4, Switzerland.
FAU - Ulm, Roman
AU  - Ulm R
AUID- ORCID: 0000-0001-8014-7392
AD  - Department of Botany and Plant Biology, University of Geneva, Sciences III, 
      CH-1211 Geneva 4, Switzerland Institute of Genetics and Genomics of Geneva 
      (iGE3), University of Geneva, CH-1211 Geneva 4, Switzerland roman.ulm@unige.ch.
LA  - eng
PT  - Journal Article
DEP - 20160328
PL  - England
TA  - Plant Cell
JT  - The Plant cell
JID - 9208688
RN  - 0 (Arabidopsis Proteins)
RN  - 0 (Chromosomal Proteins, Non-Histone)
RN  - 0 (Plant Proteins)
RN  - 0 (Uvr8 protein, Arabidopsis)
RN  - EC 2.3.2.27 (AT2G32950 protein, Arabidopsis)
RN  - EC 2.3.2.27 (Ubiquitin-Protein Ligases)
SB  - IM
CIN - Plant Cell. 2016 Apr;28(4):825. PMID: 27053422
MH  - Arabidopsis Proteins/genetics/metabolism
MH  - Chlamydomonas reinhardtii/genetics/*metabolism/*radiation effects
MH  - Chromosomal Proteins, Non-Histone/genetics/metabolism
MH  - Gene Expression Regulation, Plant/genetics/radiation effects
MH  - Plant Proteins/genetics/metabolism
MH  - Signal Transduction/radiation effects
MH  - Ubiquitin-Protein Ligases
MH  - *Ultraviolet Rays
PMC - PMC4863380
EDAT- 2016/03/30 06:00
MHDA- 2017/11/08 06:00
PMCR- 2016/03/28
CRDT- 2016/03/30 06:00
PHST- 2015/04/01 00:00 [received]
PHST- 2016/03/25 00:00 [accepted]
PHST- 2016/03/30 06:00 [entrez]
PHST- 2016/03/30 06:00 [pubmed]
PHST- 2017/11/08 06:00 [medline]
PHST- 2016/03/28 00:00 [pmc-release]
AID - tpc.15.00287 [pii]
AID - TPC201500287RAR2 [pii]
AID - 10.1105/tpc.15.00287 [doi]
PST - ppublish
SO  - Plant Cell. 2016 Apr;28(4):966-83. doi: 10.1105/tpc.15.00287. Epub 2016 Mar 28.