PMID- 27021228
OWN - NLM
STAT- MEDLINE
DCOM- 20161114
LR  - 20161230
IS  - 1480-3321 (Electronic)
IS  - 0831-2796 (Linking)
VI  - 59
IP  - 4
DP  - 2016 Apr
TI  - Molecular cytogenetic identification of a wheat-rye 1R addition line with 
      multiple spikelets and resistance to powdery mildew.
PG  - 277-88
LID - 10.1139/gen-2015-0129 [doi]
AB  - Alien addition lines are important for transferring useful genes from alien 
      species into common wheat. Rye is an important and valuable gene resource for 
      improving wheat disease resistance, yield, and environment adaptation. A new 
      wheat-rye addition line, N9436B, was developed from the progeny of the cross of 
      common wheat (Triticum aestivum L., 2n = 6x = 42, AABBDD) cultivar Shaanmai 611 
      and rye (Secale cereal L., 2n = 2x = 14, RR) accession Austrian rye. We 
      characterized this new line by cytology, genomic in situ hybridization (GISH), 
      fluorescence in situ hybridization (FISH), molecular markers, and disease 
      resistance screening. N9436B was stable in morphology and cytology, with a 
      chromosome composition of 2n = 42 + 2t = 22II. GISH investigations showed that 
      this line contained two rye chromosomes. GISH, FISH, and molecular maker 
      identification suggested that the introduced R chromosome and the missing wheat 
      chromosome arms were 1R chromosome and 2DL chromosome arm, respectively. N9436B 
      exhibited 30-37 spikelets per spike and a high level of resistance to powdery 
      mildew (Blumeria graminis f. sp. tritici, Bgt) isolate E09 at the seedling stage. 
      N9436B was cytologically stable, had the trait of multiple spikelets, and was 
      resistant to powdery mildew; this line should thus be useful in wheat 
      improvement.
FAU - Yang, Wujuan
AU  - Yang W
AD  - State Key Laboratory of Crop Stress Biology for Arid Areas, College of Agronomy, 
      Northwest A&F University, Yangling, Shaanxi 712100, China.
AD  - State Key Laboratory of Crop Stress Biology for Arid Areas, College of Agronomy, 
      Northwest A&F University, Yangling, Shaanxi 712100, China.
FAU - Wang, Changyou
AU  - Wang C
AD  - State Key Laboratory of Crop Stress Biology for Arid Areas, College of Agronomy, 
      Northwest A&F University, Yangling, Shaanxi 712100, China.
AD  - State Key Laboratory of Crop Stress Biology for Arid Areas, College of Agronomy, 
      Northwest A&F University, Yangling, Shaanxi 712100, China.
FAU - Chen, Chunhuan
AU  - Chen C
AD  - State Key Laboratory of Crop Stress Biology for Arid Areas, College of Agronomy, 
      Northwest A&F University, Yangling, Shaanxi 712100, China.
AD  - State Key Laboratory of Crop Stress Biology for Arid Areas, College of Agronomy, 
      Northwest A&F University, Yangling, Shaanxi 712100, China.
FAU - Wang, Yajuan
AU  - Wang Y
AD  - State Key Laboratory of Crop Stress Biology for Arid Areas, College of Agronomy, 
      Northwest A&F University, Yangling, Shaanxi 712100, China.
AD  - State Key Laboratory of Crop Stress Biology for Arid Areas, College of Agronomy, 
      Northwest A&F University, Yangling, Shaanxi 712100, China.
FAU - Zhang, Hong
AU  - Zhang H
AD  - State Key Laboratory of Crop Stress Biology for Arid Areas, College of Agronomy, 
      Northwest A&F University, Yangling, Shaanxi 712100, China.
AD  - State Key Laboratory of Crop Stress Biology for Arid Areas, College of Agronomy, 
      Northwest A&F University, Yangling, Shaanxi 712100, China.
FAU - Liu, Xinlun
AU  - Liu X
AD  - State Key Laboratory of Crop Stress Biology for Arid Areas, College of Agronomy, 
      Northwest A&F University, Yangling, Shaanxi 712100, China.
AD  - State Key Laboratory of Crop Stress Biology for Arid Areas, College of Agronomy, 
      Northwest A&F University, Yangling, Shaanxi 712100, China.
FAU - Ji, Wanquan
AU  - Ji W
AD  - State Key Laboratory of Crop Stress Biology for Arid Areas, College of Agronomy, 
      Northwest A&F University, Yangling, Shaanxi 712100, China.
AD  - State Key Laboratory of Crop Stress Biology for Arid Areas, College of Agronomy, 
      Northwest A&F University, Yangling, Shaanxi 712100, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20160224
PL  - Canada
TA  - Genome
JT  - Genome
JID - 8704544
RN  - 0 (Genetic Markers)
SB  - IM
MH  - Ascomycota
MH  - Chromosomes, Plant/genetics
MH  - Cytogenetic Analysis
MH  - Disease Resistance/*genetics
MH  - Genetic Markers
MH  - Hybridization, Genetic
MH  - Karyotype
MH  - Plant Breeding
MH  - Plant Diseases/*genetics/microbiology
MH  - Secale/*genetics/microbiology
MH  - Triticum/*genetics/microbiology
OTO - NOTNLM
OT  - GISH and FISH
OT  - GISH et FISH
OT  - lignee d'addition ble-seigle
OT  - marqueurs moleculaires
OT  - molecular makers
OT  - multiple spikelets
OT  - powdery mildew resistance
OT  - resistance au blanc
OT  - wheat-rye addition line
OT  - epillets multiples
EDAT- 2016/03/30 06:00
MHDA- 2016/11/15 06:00
CRDT- 2016/03/30 06:00
PHST- 2016/03/30 06:00 [entrez]
PHST- 2016/03/30 06:00 [pubmed]
PHST- 2016/11/15 06:00 [medline]
AID - 10.1139/gen-2015-0129 [doi]
PST - ppublish
SO  - Genome. 2016 Apr;59(4):277-88. doi: 10.1139/gen-2015-0129. Epub 2016 Feb 24.