PMID- 27021637
OWN - NLM
STAT- MEDLINE
DCOM- 20170907
LR  - 20181202
IS  - 1432-0436 (Electronic)
IS  - 0301-4681 (Linking)
VI  - 92
IP  - 1-2
DP  - 2016 Jul-Aug
TI  - Mouse bone marrow stromal cells differentiate to neuron-like cells upon 
      inhibition of BMP signaling.
PG  - 1-9
LID - S0301-4681(15)30066-9 [pii]
LID - 10.1016/j.diff.2016.03.003 [doi]
AB  - Bone marrow stromal cells (BMSCs) are a source of autologous stem cells that have 
      the potential for undergoing differentiation into multiple cell types including 
      neurons. Although the neuronal differentiation of mesenchymal stem cells has been 
      studied for a long time, the molecular players involved are still not defined. 
      Here we report that the genetic deletion of two members of the bone morphogenetic 
      protein (Bmp) family, Bmp2 and Bmp4 in mouse BMSCs causes their differentiation 
      into cells with neuron-like morphology. Surprisingly these cells expressed 
      certain markers characteristic of both neuronal and glial cells. Based on this 
      observation, we inhibited BMP signaling in mouse BMSCs through a brief exposure 
      to Noggin protein which also led to their differentiation into cells expressing 
      both neuronal and glial markers. Such cells seem to have the potential for 
      further differentiation into subtypes of neuronal and glial cells and thus could 
      be utilized for cell-based therapeutic applications.
CI  - Copyright (c) 2016 International Society of Differentiation. Published by Elsevier 
      B.V. All rights reserved.
FAU - Saxena, Monika
AU  - Saxena M
AD  - Department of Biological Sciences and Bioengineering, Indian Institute of 
      Technology Kanpur, Kanpur 208016, Uttar Pradesh, India.
FAU - Prashar, Paritosh
AU  - Prashar P
AD  - Department of Biological Sciences and Bioengineering, Indian Institute of 
      Technology Kanpur, Kanpur 208016, Uttar Pradesh, India.
FAU - Yadav, Prem Swaroop
AU  - Yadav PS
AD  - Department of Biological Sciences and Bioengineering, Indian Institute of 
      Technology Kanpur, Kanpur 208016, Uttar Pradesh, India.
FAU - Sen, Jonaki
AU  - Sen J
AD  - Department of Biological Sciences and Bioengineering, Indian Institute of 
      Technology Kanpur, Kanpur 208016, Uttar Pradesh, India. Electronic address: 
      jonaki@iitk.ac.in.
LA  - eng
PT  - Journal Article
DEP - 20160325
PL  - England
TA  - Differentiation
JT  - Differentiation; research in biological diversity
JID - 0401650
RN  - 0 (Bmp2 protein, mouse)
RN  - 0 (Bmp4 protein, mouse)
RN  - 0 (Bone Morphogenetic Protein 2)
RN  - 0 (Bone Morphogenetic Protein 4)
RN  - 0 (Carrier Proteins)
RN  - 0 (RNA, Messenger)
RN  - 148294-77-3 (noggin protein)
SB  - IM
EIN - Differentiation. 2020 Nov - Dec;116:38. PMID: 31983490
MH  - Animals
MH  - Blotting, Western
MH  - Bone Morphogenetic Protein 2/antagonists & inhibitors/*physiology
MH  - Bone Morphogenetic Protein 4/antagonists & inhibitors/*physiology
MH  - Carrier Proteins/genetics/*metabolism
MH  - *Cell Differentiation
MH  - Cell Proliferation
MH  - Cells, Cultured
MH  - Gene Expression Regulation
MH  - Mesenchymal Stem Cells/*cytology/metabolism
MH  - Mice
MH  - Mice, Knockout
MH  - Neurons/*cytology/metabolism
MH  - RNA, Messenger/genetics
MH  - Real-Time Polymerase Chain Reaction
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Signal Transduction
MH  - Stem Cells/*cytology/metabolism
OTO - NOTNLM
OT  - BMP
OT  - Bone marrow stromal cells
OT  - Neuron-like cells
OT  - Noggin
EDAT- 2016/03/30 06:00
MHDA- 2017/09/08 06:00
CRDT- 2016/03/30 06:00
PHST- 2015/10/22 00:00 [received]
PHST- 2016/03/06 00:00 [revised]
PHST- 2016/03/17 00:00 [accepted]
PHST- 2016/03/30 06:00 [entrez]
PHST- 2016/03/30 06:00 [pubmed]
PHST- 2017/09/08 06:00 [medline]
AID - S0301-4681(15)30066-9 [pii]
AID - 10.1016/j.diff.2016.03.003 [doi]
PST - ppublish
SO  - Differentiation. 2016 Jul-Aug;92(1-2):1-9. doi: 10.1016/j.diff.2016.03.003. Epub 
      2016 Mar 25.