PMID- 27021681
OWN - NLM
STAT- MEDLINE
DCOM- 20160829
LR  - 20160418
IS  - 1090-2104 (Electronic)
IS  - 0006-291X (Linking)
VI  - 473
IP  - 2
DP  - 2016 Apr 29
TI  - Regulator of G-protein signaling 5 protects cardiomyocytes against apoptosis 
      during in vitro cardiac ischemia-reperfusion in mice by inhibiting both JNK1/2 
      and P38 signaling pathways.
PG  - 551-7
LID - S0006-291X(16)30427-2 [pii]
LID - 10.1016/j.bbrc.2016.03.114 [doi]
AB  - Ischemic heart disease is one of the most common diseases in modern society. 
      Ischemic myocardium can be salvaged by vascular recanalization therapy, but its 
      benefit is attenuated by injury that can occur during reperfusion. And apoptotic 
      cell death plays an important part in myocardial ischemia-reperfusion (IR) 
      injury. Regulator of G-protein signaling 5 (RGS5), highly expressed in different 
      cell types of the human adult heart, is a guanosine triphosphatase-activating 
      protein to inhibit many signaling pathways such as c-Jun NH2-terminal kinase 1/2 
      (JNK1/2) and p38 which promote cardiac IR-induced apoptosis. However the role of 
      RGS5 in cardiac IR-induced apoptosis remains unclear. An in vitro IR model was 
      applied to the isolated hearts of wild type mice (WT), RGS5-transgenic mice (TG), 
      and RGS5-knockout mice (KO). Our results revealed that compared with either WT or 
      KO mice, TG mice showed inhibition of cardiomyocyte apoptosis as indicated by a 
      greater increase of B cell lymphoma/lewkmia-2 (Bcl-2), and an obvious reduction 
      in the positive expression of the terminal deoxynucleotidyl transferase-mediated 
      dUTP nick end labeling (TUNEL), Bcl-2 Associated X protein (Bax), and active 
      caspase-3. Moreover, the inhibition of both JNK1/2 and p38 signaling markedly 
      reversed IR-induced cardiomyocyte apoptosis in RGS5-KO mice. These studies show 
      that RGS5 protects cardiomyocytes against apoptosis during IR through inhibiting 
      both JNK1/2 and p38 signaling pathways.
CI  - Copyright (c) 2016 Elsevier Inc. All rights reserved.
FAU - Wang, Zhiqiang
AU  - Wang Z
AD  - Department of Cardiology, Renmin Hospital of Wuhan University, Wuhan, Hubei 
      Province, China; Cardiovascular Research Institute of Wuhan University, Wuhan, 
      Hubei Province, China.
FAU - Huang, He
AU  - Huang H
AD  - Department of Cardiology, Renmin Hospital of Wuhan University, Wuhan, Hubei 
      Province, China; Cardiovascular Research Institute of Wuhan University, Wuhan, 
      Hubei Province, China. Electronic address: wzqqzw10001@hotmail.com.
FAU - He, Wangwei
AU  - He W
AD  - Department of Cardiology, Renmin Hospital of Wuhan University, Wuhan, Hubei 
      Province, China; Cardiovascular Research Institute of Wuhan University, Wuhan, 
      Hubei Province, China.
FAU - Kong, Bin
AU  - Kong B
AD  - Department of Cardiology, Renmin Hospital of Wuhan University, Wuhan, Hubei 
      Province, China; Cardiovascular Research Institute of Wuhan University, Wuhan, 
      Hubei Province, China.
FAU - Hu, He
AU  - Hu H
AD  - Department of Cardiology, Renmin Hospital of Wuhan University, Wuhan, Hubei 
      Province, China; Cardiovascular Research Institute of Wuhan University, Wuhan, 
      Hubei Province, China.
FAU - Fan, Yang
AU  - Fan Y
AD  - Department of Cardiology, Renmin Hospital of Wuhan University, Wuhan, Hubei 
      Province, China; Cardiovascular Research Institute of Wuhan University, Wuhan, 
      Hubei Province, China.
FAU - Liao, Jiafen
AU  - Liao J
AD  - Department of Cardiology, Renmin Hospital of Wuhan University, Wuhan, Hubei 
      Province, China; Cardiovascular Research Institute of Wuhan University, Wuhan, 
      Hubei Province, China.
FAU - Wang, Lei
AU  - Wang L
AD  - Department of Cardiology, Renmin Hospital of Wuhan University, Wuhan, Hubei 
      Province, China; Cardiovascular Research Institute of Wuhan University, Wuhan, 
      Hubei Province, China.
FAU - Mei, Yang
AU  - Mei Y
AD  - Department of Cardiology, Renmin Hospital of Wuhan University, Wuhan, Hubei 
      Province, China; Cardiovascular Research Institute of Wuhan University, Wuhan, 
      Hubei Province, China.
FAU - Liu, Wanli
AU  - Liu W
AD  - Department of Cardiology, Renmin Hospital of Wuhan University, Wuhan, Hubei 
      Province, China; Cardiovascular Research Institute of Wuhan University, Wuhan, 
      Hubei Province, China.
FAU - Xiong, Xiaoju
AU  - Xiong X
AD  - Department of Cardiology, Renmin Hospital of Wuhan University, Wuhan, Hubei 
      Province, China; Cardiovascular Research Institute of Wuhan University, Wuhan, 
      Hubei Province, China.
FAU - Peng, Jianye
AU  - Peng J
AD  - Department of Cardiology, Renmin Hospital of Wuhan University, Wuhan, Hubei 
      Province, China; Cardiovascular Research Institute of Wuhan University, Wuhan, 
      Hubei Province, China.
FAU - Xiao, Yali
AU  - Xiao Y
AD  - Department of Cardiology, Renmin Hospital of Wuhan University, Wuhan, Hubei 
      Province, China; Cardiovascular Research Institute of Wuhan University, Wuhan, 
      Hubei Province, China.
FAU - Huang, Dan
AU  - Huang D
AD  - Department of Cardiology, Renmin Hospital of Wuhan University, Wuhan, Hubei 
      Province, China; Cardiovascular Research Institute of Wuhan University, Wuhan, 
      Hubei Province, China.
FAU - Quan, Dajun
AU  - Quan D
AD  - Department of Cardiology, Renmin Hospital of Wuhan University, Wuhan, Hubei 
      Province, China; Cardiovascular Research Institute of Wuhan University, Wuhan, 
      Hubei Province, China.
FAU - Li, Qi
AU  - Li Q
AD  - Department of Cardiology, Renmin Hospital of Wuhan University, Wuhan, Hubei 
      Province, China; Cardiovascular Research Institute of Wuhan University, Wuhan, 
      Hubei Province, China.
FAU - Xiong, Liang
AU  - Xiong L
AD  - Department of Cardiology, Renmin Hospital of Wuhan University, Wuhan, Hubei 
      Province, China; Cardiovascular Research Institute of Wuhan University, Wuhan, 
      Hubei Province, China.
FAU - Zhong, Peng
AU  - Zhong P
AD  - Department of Cardiology, Renmin Hospital of Wuhan University, Wuhan, Hubei 
      Province, China; Cardiovascular Research Institute of Wuhan University, Wuhan, 
      Hubei Province, China.
FAU - Wang, Guangji
AU  - Wang G
AD  - Department of Cardiology, Renmin Hospital of Wuhan University, Wuhan, Hubei 
      Province, China; Cardiovascular Research Institute of Wuhan University, Wuhan, 
      Hubei Province, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20160325
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (RGS Proteins)
RN  - 0 (Rgs5 protein, mouse)
RN  - EC 2.7.1.24 (Mitogen-Activated Protein Kinase 9)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinase 8)
SB  - IM
MH  - Animals
MH  - Apoptosis
MH  - *MAP Kinase Signaling System
MH  - Male
MH  - Mice, Inbred C57BL
MH  - Mice, Knockout
MH  - Mitogen-Activated Protein Kinase 8/*metabolism
MH  - Mitogen-Activated Protein Kinase 9/*metabolism
MH  - Myocardial Ischemia/genetics/*metabolism/pathology
MH  - Myocytes, Cardiac/metabolism/*pathology
MH  - RGS Proteins/genetics/*metabolism
MH  - *Signal Transduction
OTO - NOTNLM
OT  - Apoptosis
OT  - Ischemia-reperfusion
OT  - JNK1/2
OT  - RGS5
OT  - p38
EDAT- 2016/03/30 06:00
MHDA- 2016/08/30 06:00
CRDT- 2016/03/30 06:00
PHST- 2016/03/23 00:00 [received]
PHST- 2016/03/23 00:00 [accepted]
PHST- 2016/03/30 06:00 [entrez]
PHST- 2016/03/30 06:00 [pubmed]
PHST- 2016/08/30 06:00 [medline]
AID - S0006-291X(16)30427-2 [pii]
AID - 10.1016/j.bbrc.2016.03.114 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 2016 Apr 29;473(2):551-7. doi: 
      10.1016/j.bbrc.2016.03.114. Epub 2016 Mar 25.