PMID- 27022441
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20160329
LR  - 20200930
IS  - 1948-0210 (Print)
IS  - 1948-0210 (Electronic)
IS  - 1948-0210 (Linking)
VI  - 8
IP  - 3
DP  - 2016 Mar 26
TI  - Updates in the pathophysiological mechanisms of Parkinson's disease: Emerging 
      role of bone marrow mesenchymal stem cells.
PG  - 106-17
LID - 10.4252/wjsc.v8.i3.106 [doi]
AB  - AIM: To explore the approaches exerted by mesenchymal stem cells (MSCs) to 
      improve Parkinson's disease (PD) pathophysiology. METHODS: MSCs were harvested 
      from bone marrow of femoral bones of male rats, grown and propagated in culture. 
      Twenty four ovariectomized animals were classified into 3 groups: Group (1) was 
      control, Groups (2) and (3) were subcutaneously administered with rotenone for 14 
      d after one month of ovariectomy for induction of PD. Then, Group (2) was left 
      untreated, while Group (3) was treated with single intravenous dose of bone 
      marrow derived MSCs (BM-MSCs). SRY gene was assessed by PCR in brain tissue of 
      the female rats. Serum transforming growth factor beta-1 (TGF-beta1), monocyte 
      chemoattractant protein-1 (MCP-1) and brain derived neurotrophic factor (BDNF) 
      levels were assayed by ELISA. Brain dopamine DA level was assayed 
      fluorometrically, while brain tyrosine hydroxylase (TH) and nestin gene 
      expression were detected by semi-quantitative real time PCR. Brain survivin 
      expression was determined by immunohistochemical procedure. Histopathological 
      investigation of brain tissues was also done. RESULTS: BM-MSCs were able to home 
      at the injured brains and elicited significant decrease in serum TGF-beta1 (489.7 +/- 
      13.0 vs 691.2 +/- 8.0, P < 0.05) and MCP-1 (89.6 +/- 2.0 vs 112.1 +/- 1.9, P < 0.05) 
      levels associated with significant increase in serum BDNF (3663 +/- 17.8 vs 2905 +/- 
      72.9, P < 0.05) and brain DA (874 +/- 15.0 vs 599 +/- 9.8, P < 0.05) levels as well 
      as brain TH (1.18 +/- 0.004 vs 0.54 +/- 0.009, P < 0.05) and nestin (1.29 +/- 0.005 vs 
      0.67 +/- 0.006, P < 0.05) genes expression levels. In addition to, producing 
      insignificant increase in the number of positive cells for survivin (293.2 +/- 15.9 
      vs 271.5 +/- 15.9, P > 0.05) expression. Finally, the brain sections showed intact 
      histological structure of the striatum as a result of treatment with BM-MSCs. 
      CONCLUSION: The current study sheds light on the therapeutic potential of BM-MSCs 
      against PD pathophysiology via multi-mechanistic actions.
FAU - Ahmed, Hanaa H
AU  - Ahmed HH
AD  - Hanaa H Ahmed, Emad F Eskandar, Hadeer A Aglan, Hormones Department, Medical 
      Research Division, National Research Centre, Giza 12622, Egypt.
FAU - Salem, Ahmed M
AU  - Salem AM
AD  - Hanaa H Ahmed, Emad F Eskandar, Hadeer A Aglan, Hormones Department, Medical 
      Research Division, National Research Centre, Giza 12622, Egypt.
FAU - Atta, Hazem M
AU  - Atta HM
AD  - Hanaa H Ahmed, Emad F Eskandar, Hadeer A Aglan, Hormones Department, Medical 
      Research Division, National Research Centre, Giza 12622, Egypt.
FAU - Eskandar, Emad F
AU  - Eskandar EF
AD  - Hanaa H Ahmed, Emad F Eskandar, Hadeer A Aglan, Hormones Department, Medical 
      Research Division, National Research Centre, Giza 12622, Egypt.
FAU - Farrag, Abdel Razik H
AU  - Farrag AR
AD  - Hanaa H Ahmed, Emad F Eskandar, Hadeer A Aglan, Hormones Department, Medical 
      Research Division, National Research Centre, Giza 12622, Egypt.
FAU - Ghazy, Mohamed A
AU  - Ghazy MA
AD  - Hanaa H Ahmed, Emad F Eskandar, Hadeer A Aglan, Hormones Department, Medical 
      Research Division, National Research Centre, Giza 12622, Egypt.
FAU - Salem, Neveen A
AU  - Salem NA
AD  - Hanaa H Ahmed, Emad F Eskandar, Hadeer A Aglan, Hormones Department, Medical 
      Research Division, National Research Centre, Giza 12622, Egypt.
FAU - Aglan, Hadeer A
AU  - Aglan HA
AD  - Hanaa H Ahmed, Emad F Eskandar, Hadeer A Aglan, Hormones Department, Medical 
      Research Division, National Research Centre, Giza 12622, Egypt.
LA  - eng
PT  - Journal Article
PL  - United States
TA  - World J Stem Cells
JT  - World journal of stem cells
JID - 101535826
PMC - PMC4807309
OTO - NOTNLM
OT  - Anti-apoptotic effect
OT  - Anti-inflammatory action
OT  - Bone marrow derived mesenchymal stem cells
OT  - Neurogenic potential
OT  - Ovariectomy
OT  - Parkinson's disease
OT  - Pathophysiology
OT  - Rotenone
EDAT- 2016/03/30 06:00
MHDA- 2016/03/30 06:01
PMCR- 2016/03/26
CRDT- 2016/03/30 06:00
PHST- 2015/09/12 00:00 [received]
PHST- 2016/02/01 00:00 [revised]
PHST- 2016/02/23 00:00 [accepted]
PHST- 2016/03/30 06:00 [entrez]
PHST- 2016/03/30 06:00 [pubmed]
PHST- 2016/03/30 06:01 [medline]
PHST- 2016/03/26 00:00 [pmc-release]
AID - 10.4252/wjsc.v8.i3.106 [doi]
PST - ppublish
SO  - World J Stem Cells. 2016 Mar 26;8(3):106-17. doi: 10.4252/wjsc.v8.i3.106.