PMID- 27022730 OWN - NLM STAT- MEDLINE DCOM- 20180615 LR - 20220330 IS - 1993-0402 (Electronic) IS - 1672-0415 (Linking) VI - 23 IP - 9 DP - 2017 Sep TI - Huannao Yicong Decoction () extract reduces inflammation and cell apoptosis in Abeta(1-42)-induced Alzheimer's disease model of rats. PG - 672-680 LID - 10.1007/s11655-016-2255-1 [doi] AB - OBJECTIVE: Huannao Yicong Decoction (, HYD), an effective herbal formula against Alzheimer's disease (AD), has been proven to have neuroprotective action in amyloid beta-protein(1-42) (Abeta(1-42))-induced rat model. This study was designed to characterize mechanisms by which HYD leads to suppression of inflflammation and apoptosis in the brains of Abeta(1-42)-induced rat. METHODS: A total of 72 rats were divided into 6 groups, which were referred to as: sham operation group, model group, donepezil-treated group, HYD low-dose group (HYDL), HYD middle-dose group (HYDM) and HYD high-dose group (HYDH). Rats in HYDL, HYDM and HYDH were injected with Abeta(1-42) at the CA1 region of hippocampus to form AD model and were fed the HYD extract at different dose of 3.78, 7.56 and 18.90 g crude drug/kg. The behavioral changes of rats were evaluated by Morris water maze (MWM) before sacrififice. Pathological changes of the brain tissue were evaluated using hematoxylin eosin (HE) staining. The levels of interleukin-1 (IL-1) and tumor necrosis factor-alpha (TNF-alpha) were measured by radioimmunoassay. The levels of Abeta and proteins that are associated with apoptosis such as B-cell lymphoma-2 protein (Bcl-2), Bcl-2-associated X protein (Bax), cysteine-aspartic protease (caspase)-3, -8, -9 and -12 in serum were measured by immunohistochemistry. RESULTS: Compared with the sham operation group, the spatial learning and memory abilities of AD rats were signifificantly decreased (P<0.05 or P<0.01; Expressions of IL-1, TNF-alpha, Abeta and apoptosis-signaling proteins caspase-3, -8, -9, -12 were signifificantly up-regulated (P<0.05 or P<0.01). The ratio of Bcl-2 to Bax were signifificantly decreased in the model group (P<0.01). When treated with HYD extract, the spatial learning and memory abilities of AD-model rats were significantly increased (P<0.05 or P<0.01), IL-1, TNF-alpha, Abeta, caspase-3, -8, -9 and -12 were down-regulated (P<0.05 or P<0.01), and the ratio of Bcl-2 to Bax were reduced (P<0.05 or P<0.01). CONCLUSIONS: HYD extract can improve the learning and memory ability defificits, alleviate the inflflammatory response and pathological manifestations induced by Abeta(1-42) injection in the rat model of AD. HYD down-regulates the levels of IL-1, TNF-alpha and Abeta, and decreases the rate of apoptosis by modulating apoptosis-signaling-related proteins such as caspase-3, -8, -9, and -12. FAU - Wang, Qi AU - Wang Q AD - Gerontology Department, Xiyuan Hospital, China Academy of Chinese Medical Sciences, Beijing, 100091, China. AD - Graduate School, Beijing University of Chinese Medicine, Beijing, 100029, China. FAU - Li, Hao AU - Li H AD - Gerontology Department, Xiyuan Hospital, China Academy of Chinese Medical Sciences, Beijing, 100091, China. xyhplihao1965@126.com. FAU - Wang, Fei-Xue AU - Wang FX AD - Gerontology Department, Xiyuan Hospital, China Academy of Chinese Medical Sciences, Beijing, 100091, China. FAU - Gao, Lei AU - Gao L AD - Gerontology Department, Xiyuan Hospital, China Academy of Chinese Medical Sciences, Beijing, 100091, China. FAU - Qin, Ji-Chang AU - Qin JC AD - Gerontology Department, Xiyuan Hospital, China Academy of Chinese Medical Sciences, Beijing, 100091, China. FAU - Liu, Jian-Gang AU - Liu JG AD - Gerontology Department, Xiyuan Hospital, China Academy of Chinese Medical Sciences, Beijing, 100091, China. FAU - Wei, Yun AU - Wei Y AD - Gerontology Department, Xiyuan Hospital, China Academy of Chinese Medical Sciences, Beijing, 100091, China. FAU - Liu, Mei-Xia AU - Liu MX AD - Gerontology Department, Xiyuan Hospital, China Academy of Chinese Medical Sciences, Beijing, 100091, China. LA - eng PT - Journal Article DEP - 20160329 PL - China TA - Chin J Integr Med JT - Chinese journal of integrative medicine JID - 101181180 RN - 0 (Amyloid beta-Peptides) RN - 0 (Drugs, Chinese Herbal) RN - 0 (Interleukin-1) RN - 0 (Peptide Fragments) RN - 0 (Plant Extracts) RN - 0 (Tumor Necrosis Factor-alpha) RN - 0 (amyloid beta-protein (1-42)) RN - 0 (bcl-2-Associated X Protein) RN - 0 (huannao yicong) RN - EC 3.4.22.- (Caspases) SB - IM MH - Alzheimer Disease/blood/complications/*drug therapy/*pathology MH - Amyloid beta-Peptides MH - Animals MH - *Apoptosis/drug effects MH - Caspases/metabolism MH - Chromatography, High Pressure Liquid MH - Disease Models, Animal MH - Drugs, Chinese Herbal/*therapeutic use MH - Female MH - Hippocampus/drug effects/pathology MH - Inflammation/blood/complications/*drug therapy/*pathology MH - Interleukin-1/blood/metabolism MH - Male MH - Peptide Fragments MH - Plant Extracts/*therapeutic use MH - Rats, Sprague-Dawley MH - Spatial Learning/drug effects MH - Tumor Necrosis Factor-alpha/blood/metabolism MH - bcl-2-Associated X Protein/metabolism OTO - NOTNLM OT - Alzheimer's disease OT - Chinese medicine compound OT - Huannao Yicong Decoction OT - apoptosis OT - inflammation EDAT- 2016/03/30 06:00 MHDA- 2018/06/16 06:00 CRDT- 2016/03/30 06:00 PHST- 2015/11/11 00:00 [received] PHST- 2016/03/30 06:00 [pubmed] PHST- 2018/06/16 06:00 [medline] PHST- 2016/03/30 06:00 [entrez] AID - 10.1007/s11655-016-2255-1 [pii] AID - 10.1007/s11655-016-2255-1 [doi] PST - ppublish SO - Chin J Integr Med. 2017 Sep;23(9):672-680. doi: 10.1007/s11655-016-2255-1. Epub 2016 Mar 29.