PMID- 27023515
OWN - NLM
STAT- MEDLINE
DCOM- 20161214
LR  - 20220331
IS  - 1422-0067 (Electronic)
IS  - 1422-0067 (Linking)
VI  - 17
IP  - 4
DP  - 2016 Mar 23
TI  - Monocyte Subsets and Related Chemokines in Carotid Artery Stenosis and Ischemic 
      Stroke.
PG  - 433
LID - 10.3390/ijms17040433 [doi]
LID - 433
AB  - Carotid stenosis (CS) is an important cause of ischemic stroke. However, reliable 
      markers for the purpose of identification of high-risk, so-called vulnerable 
      carotid plaques, are still lacking. Monocyte subsets are crucial players in 
      atherosclerosis and might also contribute to plaque rupture. In this study we, 
      therefore, aimed to investigate the potential role of monocyte subsets and 
      associated chemokines as clinical biomarkers for vulnerability of CS. Patients 
      with symptomatic and asymptomatic CS (n = 21), patients with cardioembolic 
      ischemic strokes (n = 11), and controls without any cardiovascular disorder (n = 
      11) were examined. Cardiovascular risk was quantified using the Essen Stroke Risk 
      Score (ESRS). Monocyte subsets in peripheral blood were measured by quantitative 
      flow cytometry. Plaque specimens were histologically analyzed. Furthermore, 
      plasma levels of monocyte chemotactic protein 1 (MCP-1) and fractalkine were 
      measured. Intermediate monocytes (Mon2) were significantly elevated in 
      symptomatic and asymptomatic CS-patients compared to controls. Mon2 counts 
      positively correlated with the ESRS. Moreover, stroke patients showed an 
      elevation of Mon2 compared to controls, independent of the ESRS. MCP-1 levels 
      were significantly higher in patients with symptomatic than in those with 
      asymptomatic CS. Several histological criteria significantly differed between 
      symptomatic and asymptomatic plaques. However, there was no association of 
      monocyte subsets or chemokines with histological features of plaque 
      vulnerability. Due to the multifactorial influence on monocyte subsets, the 
      usability as clinical markers for plaque vulnerability seems to be limited. 
      However, monocyte subsets may be critically involved in the pathology of CS.
FAU - Grosse, Gerrit M
AU  - Grosse GM
AD  - Department of Neurology, Hannover Medical School, 30625 Hannover, Germany. 
      grosse.gerrit@mh-hannover.de.
FAU - Schulz-Schaeffer, Walter J
AU  - Schulz-Schaeffer WJ
AD  - Department of Neuropathology, University Medical Center Gottingen, 37099 
      Gottingen, Germany. wjschulz@med.uni-goettingen.de.
FAU - Teebken, Omke E
AU  - Teebken OE
AD  - Department of Vascular Surgery, Klinikum Peine, 31226 Peine, Germany. 
      omke.teebken@klinikum-peine.de.
FAU - Schuppner, Ramona
AU  - Schuppner R
AD  - Department of Neurology, Hannover Medical School, 30625 Hannover, Germany. 
      schuppner.ramona@mh-hannover.de.
FAU - Dirks, Meike
AU  - Dirks M
AD  - Department of Neurology, Hannover Medical School, 30625 Hannover, Germany. 
      dirks.meike@mh-hannover.de.
FAU - Worthmann, Hans
AU  - Worthmann H
AD  - Department of Neurology, Hannover Medical School, 30625 Hannover, Germany. 
      worthmann.hans@mh-hannover.de.
FAU - Lichtinghagen, Ralf
AU  - Lichtinghagen R
AD  - Department of Clinical Chemistry, Hannover Medical School, 30625 Hannover, 
      Germany. lichtinghagen.ralf@mh-hannover.de.
FAU - Maye, Gerrit
AU  - Maye G
AD  - Department of Nephrology and Hypertension, Hannover Medical School, 30625 
      Hannover, Germany. gerritmaye@gmail.com.
FAU - Limbourg, Florian P
AU  - Limbourg FP
AD  - Department of Nephrology and Hypertension, Hannover Medical School, 30625 
      Hannover, Germany. limbourg.florian@mh-hannover.de.
FAU - Weissenborn, Karin
AU  - Weissenborn K
AD  - Department of Neurology, Hannover Medical School, 30625 Hannover, Germany. 
      Weissenborn.Karin@mh-hannover.de.
AD  - Center for Systems Neuroscience (ZSN), 30559 Hannover, Germany. 
      Weissenborn.Karin@mh-hannover.de.
LA  - eng
PT  - Journal Article
DEP - 20160323
PL  - Switzerland
TA  - Int J Mol Sci
JT  - International journal of molecular sciences
JID - 101092791
RN  - 0 (Biomarkers)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Chemokine CX3CL1)
RN  - 0 (Chemokines)
SB  - IM
MH  - Aged
MH  - Aged, 80 and over
MH  - Biomarkers/metabolism
MH  - Carotid Stenosis/complications/metabolism/*pathology
MH  - Case-Control Studies
MH  - Chemokine CCL2/blood
MH  - Chemokine CX3CL1/blood
MH  - Chemokines/*blood
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Female
MH  - Flow Cytometry
MH  - Humans
MH  - Leukocyte Count
MH  - Male
MH  - Middle Aged
MH  - Monocytes/cytology/immunology/*metabolism
MH  - Risk Factors
MH  - Stroke/epidemiology/*etiology
PMC - PMC4848889
OTO - NOTNLM
OT  - atherosclerosis
OT  - biomarkers
OT  - carotid stenosis
OT  - ischemic stroke
OT  - plaque analysis
EDAT- 2016/03/31 06:00
MHDA- 2016/12/15 06:00
PMCR- 2016/04/01
CRDT- 2016/03/30 06:00
PHST- 2016/01/30 00:00 [received]
PHST- 2016/03/08 00:00 [revised]
PHST- 2016/03/17 00:00 [accepted]
PHST- 2016/03/30 06:00 [entrez]
PHST- 2016/03/31 06:00 [pubmed]
PHST- 2016/12/15 06:00 [medline]
PHST- 2016/04/01 00:00 [pmc-release]
AID - ijms17040433 [pii]
AID - ijms-17-00433 [pii]
AID - 10.3390/ijms17040433 [doi]
PST - epublish
SO  - Int J Mol Sci. 2016 Mar 23;17(4):433. doi: 10.3390/ijms17040433.