PMID- 27023722 OWN - NLM STAT- MEDLINE DCOM- 20170315 LR - 20181113 IS - 1572-994X (Electronic) IS - 0920-8569 (Linking) VI - 52 IP - 4 DP - 2016 Aug TI - Identification and RNA segment assignment of six structural proteins of Scylla serrata reovirus. PG - 556-60 LID - 10.1007/s11262-016-1319-9 [doi] AB - Scylla serrata reovirus (SsRV) is one of the most prevalent viral pathogens of the mud crab (S. serrata). The virus represents an unassigned novel genus in the Reoviridae family, and contains 12 double-stranded RNA genomic segments. Previous analysis of virion proteins concluded that SsRV contains at least eight structural proteins, ranging from 25 to 160 kDa. Here, tandem time-of-flight mass spectrometry and Western blotting were used to re-identify the structural proteins. The results indicate that proteins encoded by SsRV segments S1, S3, S6, S9, S11, and S12 are structural proteins. FAU - Yuan, Yangyang AU - Yuan Y AD - College of Fisheries and Life Sciences, Shanghai Ocean University, Shanghai, People's Republic of China. AD - College of Biological and Environmental Sciences, Zhejiang Wanli University, No. 8, South Qianhu Road, Ningbo, 315100, Zhejiang Province, People's Republic of China. FAU - Fan, Dongyang AU - Fan D AD - College of Biological and Environmental Sciences, Zhejiang Wanli University, No. 8, South Qianhu Road, Ningbo, 315100, Zhejiang Province, People's Republic of China. FAU - Zhang, Zhao AU - Zhang Z AD - College of Biological and Environmental Sciences, Zhejiang Wanli University, No. 8, South Qianhu Road, Ningbo, 315100, Zhejiang Province, People's Republic of China. FAU - Yang, Jifang AU - Yang J AD - College of Biological and Environmental Sciences, Zhejiang Wanli University, No. 8, South Qianhu Road, Ningbo, 315100, Zhejiang Province, People's Republic of China. FAU - Liu, Jingwen AU - Liu J AD - Bioengineering College of Jimei University, Xiamen, People's Republic of China. FAU - Chen, Jigang AU - Chen J AD - College of Biological and Environmental Sciences, Zhejiang Wanli University, No. 8, South Qianhu Road, Ningbo, 315100, Zhejiang Province, People's Republic of China. genomic@163.com. LA - eng PT - Journal Article DEP - 20160329 PL - United States TA - Virus Genes JT - Virus genes JID - 8803967 RN - 0 (RNA, Double-Stranded) RN - 0 (RNA, Viral) RN - 0 (Viral Structural Proteins) SB - IM MH - Animals MH - Brachyura/*virology MH - RNA, Double-Stranded/genetics MH - RNA, Viral/*genetics MH - Reoviridae/*genetics MH - Viral Structural Proteins/*genetics MH - Virion/genetics OTO - NOTNLM OT - Mass spectrometry OT - Reovirus OT - Scylla serrata OT - Structural proteins EDAT- 2016/03/30 06:00 MHDA- 2017/03/16 06:00 CRDT- 2016/03/30 06:00 PHST- 2016/02/03 00:00 [received] PHST- 2016/03/11 00:00 [accepted] PHST- 2016/03/30 06:00 [entrez] PHST- 2016/03/30 06:00 [pubmed] PHST- 2017/03/16 06:00 [medline] AID - 10.1007/s11262-016-1319-9 [pii] AID - 10.1007/s11262-016-1319-9 [doi] PST - ppublish SO - Virus Genes. 2016 Aug;52(4):556-60. doi: 10.1007/s11262-016-1319-9. Epub 2016 Mar 29.