PMID- 27031857
OWN - NLM
STAT- MEDLINE
DCOM- 20160812
LR  - 20190219
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 11
IP  - 3
DP  - 2016
TI  - In Silico and Fluorescence In Situ Hybridization Mapping Reveals Collinearity 
      between the Pennisetum squamulatum Apomixis Carrier-Chromosome and Chromosome 2 
      of Sorghum and Foxtail Millet.
PG  - e0152411
LID - 10.1371/journal.pone.0152411 [doi]
LID - e0152411
AB  - Apomixis, or clonal propagation through seed, is a trait identified within 
      multiple species of the grass family (Poaceae). The genetic locus controlling 
      apomixis in Pennisetum squamulatum (syn Cenchrus squamulatus) and Cenchrus 
      ciliaris (syn Pennisetum ciliare, buffelgrass) is the apospory-specific genomic 
      region (ASGR). Previously, the ASGR was shown to be highly conserved but inverted 
      in marker order between P. squamulatum and C. ciliaris based on fluorescence in 
      situ hybridization (FISH) and varied in both karyotype and position of the ASGR 
      on the ASGR-carrier chromosome among other apomictic Cenchrus/Pennisetum species. 
      Using in silico transcript mapping and verification of physical positions of some 
      of the transcripts via FISH, we discovered that the ASGR-carrier chromosome from 
      P. squamulatum is collinear with chromosome 2 of foxtail millet and sorghum 
      outside of the ASGR. The in silico ordering of the ASGR-carrier chromosome 
      markers, previously unmapped in P. squamulatum, allowed for the identification of 
      a backcross line with structural changes to the P. squamulatum ASGR-carrier 
      chromosome derived from gamma irradiated pollen.
FAU - Sapkota, Sirjan
AU  - Sapkota S
AD  - Department of Horticulture, University of Georgia-Tifton Campus, Tifton, Georgia, 
      31793, United States of America.
FAU - Conner, Joann A
AU  - Conner JA
AD  - Department of Horticulture, University of Georgia-Tifton Campus, Tifton, Georgia, 
      31793, United States of America.
FAU - Hanna, Wayne W
AU  - Hanna WW
AD  - Department of Crop and Soil Sciences, University of Georgia-Tifton Campus, 
      Tifton, Georgia, 31793, United States of America.
FAU - Simon, Bindu
AU  - Simon B
AD  - Department of Horticulture, University of Georgia-Tifton Campus, Tifton, Georgia, 
      31793, United States of America.
FAU - Fengler, Kevin
AU  - Fengler K
AD  - DuPont Pioneer, DuPont Pioneer, Johnston, Iowa, 50131, United States of America.
FAU - Deschamps, Stephane
AU  - Deschamps S
AD  - DuPont Experimental Station, Wilmington, Delaware, 19803, United States of 
      America.
FAU - Cigan, Mark
AU  - Cigan M
AD  - DuPont Pioneer, DuPont Pioneer, Johnston, Iowa, 50131, United States of America.
FAU - Ozias-Akins, Peggy
AU  - Ozias-Akins P
AD  - Department of Horticulture, University of Georgia-Tifton Campus, Tifton, Georgia, 
      31793, United States of America.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20160331
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
SB  - IM
MH  - Apomixis/genetics
MH  - Chromosomes, Artificial, Bacterial
MH  - *Chromosomes, Plant
MH  - Contig Mapping
MH  - Genetic Linkage
MH  - In Situ Hybridization, Fluorescence
MH  - Pennisetum/*genetics
MH  - Setaria Plant/genetics
MH  - Sorghum/*genetics
PMC - PMC4816547
COIS- Competing Interests: The financial support of Pioneer Hi-Bred does not alter the 
      authors' adherence to PLOS ONE policies on sharing data and materials.
EDAT- 2016/04/01 06:00
MHDA- 2016/08/16 06:00
PMCR- 2016/03/31
CRDT- 2016/04/01 06:00
PHST- 2016/01/25 00:00 [received]
PHST- 2016/03/14 00:00 [accepted]
PHST- 2016/04/01 06:00 [entrez]
PHST- 2016/04/01 06:00 [pubmed]
PHST- 2016/08/16 06:00 [medline]
PHST- 2016/03/31 00:00 [pmc-release]
AID - PONE-D-16-03094 [pii]
AID - 10.1371/journal.pone.0152411 [doi]
PST - epublish
SO  - PLoS One. 2016 Mar 31;11(3):e0152411. doi: 10.1371/journal.pone.0152411. 
      eCollection 2016.