PMID- 27032379
OWN - NLM
STAT- MEDLINE
DCOM- 20170405
LR  - 20171206
IS  - 1724-6008 (Electronic)
IS  - 0393-6155 (Linking)
VI  - 31
IP  - 3
DP  - 2016 Jul 30
TI  - Expression of MMP and TIMP mRNA in peripheral blood leukocytes of patients with 
      invasive ductal carcinoma of the breast.
PG  - e309-16
LID - 2EF09187-81AB-4442-823E-8DE2A9907214 [pii]
LID - 10.5301/jbm.5000203 [doi]
AB  - PURPOSE: An imbalance between matrix metalloproteinases (MMPs) and tissue 
      inhibitors of MMPs (TIMPs) appears critical for tumor progression and metastasis. 
      This study aimed to determine whether gene expression of MMP1, MMP2, MMP9, TIMP1 
      and TIMP3 and the MMP/TIMP expression ratio in peripheral blood leukocytes (PBLs) 
      and the MMP1 and TIMP1 contents or MMP1/TIMP1 ratio in plasma were associated 
      with clinicopathological characteristics in invasive ductal carcinoma (IDC) of 
      the breast. MATERIALS AND METHODS: Blood samples were collected from women newly 
      diagnosed with IDC who had not received prior treatment (n = 102). Gene 
      expression in PBLs was analyzed by quantitative real-time polymerase chain 
      reaction. Concentrations of MMP1 and TIMP1 in plasma were measured using ELISA. 
      RESULTS: In univariate analysis the expression levels of MMP2 and TIMP1 mRNA were 
      significantly higher in premenopausal compared to postmenopausal patients 
      (p<0.001 and p = 0.014, respectively). MMP2 mRNA expression negatively 
      correlated with age (p<0.001, r = -0.43). We found that the MMP2/TIMP3 
      expression ratio was significantly higher in women after menopause (p = 0.007). 
      The MMP2/TIMP1 expression ratio was higher in human epidermal growth factor 
      receptor 2 (HER2)-positive patients (p = 0.022). Low-grade tumors had 
      significantly lower MMP1/TIMP1 and MMP2/TIMP1 expression ratios (p = 0.047 and p 
      = 0.048, respectively). TIMP1 plasma concentration was significantly higher in 
      small tumors compared with T2-T3 tumors (p = 0.013). CONCLUSIONS: These findings 
      reveal an important association between tumor characteristics and expression 
      ratios of MMP1/TIMP1 and MMP2/TIMP1 in PBLs and TIMP1 concentration in plasma. 
      Menopausal status may influence the mRNA expression levels of MMP2 and TIMP1 as 
      well as the MMP2/TIMP3 expression ratio in IDC of the breast.
FAU - Wieczorek, Edyta
AU  - Wieczorek E
AD  - Department of Toxicology and Carcinogenesis, Nofer Institute of Occupational 
      Medicine, Lodz - Poland.
FAU - Galicki, Michal
AU  - Galicki M
AD  - Copernicus Memorial Hospital, Department of Surgical Oncology, Lodz - Poland.
FAU - Tomasik, Bartlomiej
AU  - Tomasik B
AD  - Department of Pediatrics, Oncology, Hematology and Diabetology, Medical 
      University, Lodz - Poland.
FAU - Krol, Magdalena
AU  - Krol M
AD  - Department of Toxicology and Carcinogenesis, Nofer Institute of Occupational 
      Medicine, Lodz - Poland.
FAU - Jablonska, Ewa
AU  - Jablonska E
AD  - Department of Toxicology and Carcinogenesis, Nofer Institute of Occupational 
      Medicine, Lodz - Poland.
FAU - Fendler, Wojciech
AU  - Fendler W
AD  - Department of Pediatrics, Oncology, Hematology and Diabetology, Medical 
      University, Lodz - Poland.
FAU - Gromadzinska, Jolanta
AU  - Gromadzinska J
AD  - Department of Toxicology and Carcinogenesis, Nofer Institute of Occupational 
      Medicine, Lodz - Poland.
FAU - Morawiec, Zbigniew
AU  - Morawiec Z
AD  - Copernicus Memorial Hospital, Department of Surgical Oncology, Lodz - Poland.
FAU - Wasowicz, Wojciech
AU  - Wasowicz W
AD  - Department of Toxicology and Carcinogenesis, Nofer Institute of Occupational 
      Medicine, Lodz - Poland.
FAU - Reszka, Edyta
AU  - Reszka E
AD  - Department of Toxicology and Carcinogenesis, Nofer Institute of Occupational 
      Medicine, Lodz - Poland.
LA  - eng
PT  - Journal Article
DEP - 20160730
PL  - United States
TA  - Int J Biol Markers
JT  - The International journal of biological markers
JID - 8712411
RN  - 0 (RNA, Messenger)
RN  - 0 (TIMP1 protein, human)
RN  - 0 (Tissue Inhibitor of Metalloproteinase-1)
RN  - EC 3.4.24.- (Matrix Metalloproteinases)
SB  - IM
MH  - Breast Neoplasms/blood/*genetics/metabolism
MH  - Carcinoma, Ductal, Breast/blood/*genetics/metabolism
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Female
MH  - Humans
MH  - Leukocytes/cytology/*metabolism
MH  - Matrix Metalloproteinases/biosynthesis/*genetics/metabolism
MH  - Middle Aged
MH  - RNA, Messenger/*biosynthesis/blood/genetics
MH  - Tissue Inhibitor of Metalloproteinase-1/biosynthesis/*genetics/metabolism
EDAT- 2016/04/02 06:00
MHDA- 2017/04/06 06:00
CRDT- 2016/04/02 06:00
PHST- 2016/03/08 00:00 [accepted]
PHST- 2016/04/02 06:00 [entrez]
PHST- 2016/04/02 06:00 [pubmed]
PHST- 2017/04/06 06:00 [medline]
AID - 2EF09187-81AB-4442-823E-8DE2A9907214 [pii]
AID - 10.5301/jbm.5000203 [doi]
PST - epublish
SO  - Int J Biol Markers. 2016 Jul 30;31(3):e309-16. doi: 10.5301/jbm.5000203.