PMID- 27032597
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20160404
LR  - 20160401
IS  - 0022-3646 (Print)
IS  - 0022-3646 (Linking)
VI  - 45
IP  - 6
DP  - 2009 Dec
TI  - LOCALIZATION OF MICROCYSTIN SYNTHETASE GENES IN COLONIES OF THE CYANOBACTERIUM 
      MICROCYSTIS USING FLUORESCENCE IN SITU HYBRIDIZATION(1).
PG  - 1400-4
LID - 10.1111/j.1529-8817.2009.00750.x [doi]
AB  - To better understand the production of microcystins (MCs) in Microcystis 
      colonies, fluorescence in situ hybridization (FISH) methods were developed to 
      detect DNA involved in the synthesis of these cyanobacterial hepatotoxins. Using 
      colonies of Microcystis aeruginosa (Kutz.) Kutz. isolated from environmental 
      blooms of cyanobacteria and from a colony-forming, MC-producing laboratory strain 
      of Microcystis, amplified PCR products were observed, coincident with positive 
      controls. The total MC content of individual colonies of Microcystis, determined 
      by ELISA, showed a positive correlation with colony cross-sectional area. FISH 
      analysis of Microcystis colonies gave high fluorescence in comparison to negative 
      controls, indicating the presence of MC synthetase DNA (mcyA) in situ. FISH 
      analysis for MC synthetase genes has the potential to be developed into an 
      effective early warning tool for drinking and recreational water management.
CI  - (c) 2009 Phycological Society of America.
FAU - Metcalf, James S
AU  - Metcalf JS
AD  - Division of Molecular Microbiology, College of Life Sciences, University of 
      Dundee, Dundee, DD1 4HN, UK.
FAU - Reilly, Marianne
AU  - Reilly M
AD  - Division of Molecular Microbiology, College of Life Sciences, University of 
      Dundee, Dundee, DD1 4HN, UK.
FAU - Young, Fiona M
AU  - Young FM
AD  - Division of Molecular Microbiology, College of Life Sciences, University of 
      Dundee, Dundee, DD1 4HN, UK.
FAU - Codd, Geoffrey A
AU  - Codd GA
AD  - Division of Molecular Microbiology, College of Life Sciences, University of 
      Dundee, Dundee, DD1 4HN, UK.
LA  - eng
PT  - Journal Article
DEP - 20090929
PL  - United States
TA  - J Phycol
JT  - Journal of phycology
JID - 9882935
OTO - NOTNLM
OT  - DNA
OT  - ELISA
OT  - Microcystis
OT  - PCR
OT  - fluorescence in situ hybridization
OT  - microcystin
EDAT- 2009/12/01 00:00
MHDA- 2009/12/01 00:01
CRDT- 2016/04/02 06:00
PHST- 2016/04/02 06:00 [entrez]
PHST- 2009/12/01 00:00 [pubmed]
PHST- 2009/12/01 00:01 [medline]
AID - 10.1111/j.1529-8817.2009.00750.x [doi]
PST - ppublish
SO  - J Phycol. 2009 Dec;45(6):1400-4. doi: 10.1111/j.1529-8817.2009.00750.x. Epub 2009 
      Sep 29.