PMID- 27033213
OWN - NLM
STAT- MEDLINE
DCOM- 20161216
LR  - 20161217
IS  - 1940-6029 (Electronic)
IS  - 1064-3745 (Linking)
VI  - 1393
DP  - 2016
TI  - Generation and Cryopreservation of Clinical Grade Wilms' Tumor 1 mRNA-Loaded 
      Dendritic Cell Vaccines for Cancer Immunotherapy.
PG  - 27-35
LID - 10.1007/978-1-4939-3338-9_3 [doi]
AB  - First described in the 1970s, dendritic cells (DC) are currently subjects of 
      intense investigation to exploit their unique antigen-presenting and 
      immunoregulatory capacities. In cancer, DC show promise to elicit or amplify 
      immune responses directed against cancer cells by activating natural killer (NK) 
      cells and tumor antigen-specific T cells. Wilms' tumor 1 (WT1) protein is a 
      tumor-associated antigen that is expressed in a majority of cancer types and has 
      been designated as an antigen of major interest to be targeted in clinical cancer 
      immunotherapy trials. In this chapter, we describe the generation, 
      cryopreservation, and thawing of clinical grade autologous monocyte-derived DC 
      vaccines that are loaded with WT1 by messenger RNA (mRNA) electroporation. This 
      in-house-developed transfection method gives rise to presentation of multiple 
      antigen epitopes and can be used for all patients without restriction of human 
      leukocyte antigen (HLA) type.
FAU - Smits, Evelien L J M
AU  - Smits EL
AD  - Laboratory of Experimental Hematology, Vaccine and Infectious Disease Institute, 
      University of Antwerp, Campus Drie Eiken, Universiteitsplein 1, D.T.431, Wilrijk, 
      Antwerp, 2610, Belgium. evelien.smits@uza.be.
AD  - Center for Cell Therapy and Regenerative Medicine, Antwerp University Hospital, 
      Edegem, Belgium. evelien.smits@uza.be.
FAU - Stein, Barbara
AU  - Stein B
AD  - Center for Cell Therapy and Regenerative Medicine, Antwerp University Hospital, 
      Edegem, Belgium.
FAU - Nijs, Griet
AU  - Nijs G
AD  - Center for Cell Therapy and Regenerative Medicine, Antwerp University Hospital, 
      Edegem, Belgium.
FAU - Lion, Eva
AU  - Lion E
AD  - Laboratory of Experimental Hematology, Vaccine and Infectious Disease Institute, 
      University of Antwerp, Campus Drie Eiken, Universiteitsplein 1, D.T.431, Wilrijk, 
      Antwerp, 2610, Belgium.
AD  - Center for Cell Therapy and Regenerative Medicine, Antwerp University Hospital, 
      Antwerp, Belgium.
FAU - Van Tendeloo, Viggo F
AU  - Van Tendeloo VF
AD  - Laboratory of Experimental Hematology, Vaccine and Infectious Disease Institute, 
      University of Antwerp, Campus Drie Eiken, Universiteitsplein 1, D.T.431, Wilrijk, 
      Antwerp, 2610, Belgium.
FAU - Willemen, Yannick
AU  - Willemen Y
AD  - Laboratory of Experimental Hematology, Vaccine and Infectious Disease Institute, 
      University of Antwerp, Campus Drie Eiken, Universiteitsplein 1, D.T.431, Wilrijk, 
      Antwerp, 2610, Belgium.
AD  - Center for Cell Therapy and Regenerative Medicine, Antwerp University Hospital, 
      Edegem, Belgium.
FAU - Anguille, Sebastien
AU  - Anguille S
AD  - Laboratory of Experimental Hematology, Vaccine and Infectious Disease Institute, 
      University of Antwerp, Campus Drie Eiken, Universiteitsplein 1, D.T.431, Wilrijk, 
      Antwerp, 2610, Belgium.
AD  - Center for Cell Therapy and Regenerative Medicine, Antwerp University Hospital, 
      Edegem, Belgium.
FAU - Berneman, Zwi N
AU  - Berneman ZN
AD  - Laboratory of Experimental Hematology, Vaccine and Infectious Disease Institute, 
      University of Antwerp, Campus Drie Eiken, Universiteitsplein 1, D.T.431, Wilrijk, 
      Antwerp, 2610, Belgium.
AD  - Center for Cell Therapy and Regenerative Medicine, Antwerp University Hospital, 
      Edegem, Belgium.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
RN  - 0 (Cancer Vaccines)
RN  - 0 (RNA, Messenger)
RN  - 0 (WT1 Proteins)
RN  - 0 (WT1 protein, human)
SB  - IM
MH  - *Cancer Vaccines
MH  - Cells, Cultured
MH  - *Cryopreservation
MH  - *Dendritic Cells
MH  - Electroporation
MH  - Humans
MH  - *RNA, Messenger
MH  - Vaccination
MH  - WT1 Proteins/genetics
MH  - Wilms Tumor/*therapy
OTO - NOTNLM
OT  - Clinical grade
OT  - Dendritic cells
OT  - Electroporation
OT  - RNA
OT  - WT1
OT  - Wilms' tumor 1
EDAT- 2016/04/02 06:00
MHDA- 2016/12/17 06:00
CRDT- 2016/04/02 06:00
PHST- 2016/04/02 06:00 [entrez]
PHST- 2016/04/02 06:00 [pubmed]
PHST- 2016/12/17 06:00 [medline]
AID - 10.1007/978-1-4939-3338-9_3 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2016;1393:27-35. doi: 10.1007/978-1-4939-3338-9_3.