PMID- 27034039
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20160404
LR  - 20160401
IS  - 0022-3646 (Print)
IS  - 0022-3646 (Linking)
VI  - 45
IP  - 3
DP  - 2009 Jun
TI  - UREASE GENE SEQUENCES FROM ALGAE AND HETEROTROPHIC BACTERIA IN AXENIC AND 
      NONAXENIC PHYTOPLANKTON CULTURES(1).
PG  - 625-34
LID - 10.1111/j.1529-8817.2009.00680.x [doi]
AB  - While urea has long been recognized as an important form of nitrogen in 
      planktonic ecosystems, very little is known about how many or which phytoplankton 
      and bacteria can use urea as a nitrogen source. We developed a method, targeting 
      the gene encoding urease, for the direct detection and identification of 
      ureolytic organisms and tested it on seven axenic phytoplankton cultures (three 
      diatoms, two prymnesiophytes, a eustigmatophyte, and a pelagophyte) and on three 
      nonaxenic Aureococcus anophagefferens Hargraves et Sieburth cultures (CCMP1784 
      and two CCMP1708 cultures from different laboratories). The urease amplicon 
      sequences from axenic phytoplankton cultures were consistent with genomic data in 
      the three species for which both were available. Seven of 12 phytoplankton 
      species have one or more introns in the amplified region of their urease gene(s). 
      The 63 urease amplicons that were cloned and sequenced from nonaxenic A. 
      anophagefferens cultures grouped into 17 distinct sequence types. Eleven types 
      were related to alpha-Proteobacteria, including three types likely belonging to the 
      genus Roseovarius. Four types were related to gamma-Proteobacteria, including two 
      likely belonging to the genus Marinobacter, and two types were related to 
      beta-Proteobacteria. Terminal restriction fragment length polymorphism (TRFLP) 
      analyses suggested that the sequenced amplicons represented approximately half of 
      the diversity of bacterial urease genes present in the nonaxenic cultures. While 
      many of the bacterial urease sequence types were apparently lab- or 
      culture-specific, others were found in all three nonaxenic cultures, suggesting 
      the possibility of specific relationships between these bacteria and A. 
      anophagefferens.
CI  - (c) 2009 Phycological Society of America.
FAU - Baker, Kristopher M
AU  - Baker KM
AD  - School of Marine and Atmospheric Sciences, Stony Brook University, Stony Brook, 
      New York 11794-5000, USA.
FAU - Gobler, Christopher J
AU  - Gobler CJ
AD  - School of Marine and Atmospheric Sciences, Stony Brook University, Stony Brook, 
      New York 11794-5000, USA.
FAU - Collier, Jackie L
AU  - Collier JL
AD  - School of Marine and Atmospheric Sciences, Stony Brook University, Stony Brook, 
      New York 11794-5000, USA.
LA  - eng
SI  - GENBANK/AF432598
SI  - GENBANK/AF432601
SI  - GENBANK/DQ642537
SI  - GENBANK/DQ642599
SI  - GENBANK/DQ642600
SI  - GENBANK/EU520523
SI  - GENBANK/EU520524
SI  - GENBANK/EU520525
SI  - GENBANK/EU520526
PT  - Journal Article
DEP - 20090521
PL  - United States
TA  - J Phycol
JT  - Journal of phycology
JID - 9882935
OTO - NOTNLM
OT  - Aureococcus anophagefferens
OT  - Nannochloropsis gaditana
OT  - Phaeodactylum tricornutum
OT  - Pseudoisochrysis paradoxa
OT  - Thalassiosira oceanica
OT  - Thalassiosira pseudonana
OT  - algal-bacterial interactions
OT  - intron
OT  - urea
OT  - urease
EDAT- 2009/06/01 00:00
MHDA- 2009/06/01 00:01
CRDT- 2016/04/02 06:00
PHST- 2016/04/02 06:00 [entrez]
PHST- 2009/06/01 00:00 [pubmed]
PHST- 2009/06/01 00:01 [medline]
AID - 10.1111/j.1529-8817.2009.00680.x [doi]
PST - ppublish
SO  - J Phycol. 2009 Jun;45(3):625-34. doi: 10.1111/j.1529-8817.2009.00680.x. Epub 2009 
      May 21.