PMID- 27035356
OWN - NLM
STAT- MEDLINE
DCOM- 20170510
LR  - 20210108
IS  - 1097-4652 (Electronic)
IS  - 0021-9541 (Linking)
VI  - 232
IP  - 1
DP  - 2017 Jan
TI  - MG132 Induces Expression of Monocyte Chemotactic Protein-Induced Protein 1 in 
      Vascular Smooth Muscle Cells.
PG  - 122-8
LID - 10.1002/jcp.25396 [doi]
AB  - Monocyte chemoattractant protein-1 (MCP-1) has been reported to induce the 
      expression of monocyte chemotactic protein-induced protein 1 (MCPIP1), which 
      undergoes ubiquitination degradation. Therefore, we predict that in vascular 
      smooth muscle (VSMCs), MCPIP1 may be induced by MCP-1 and undergo degradation, 
      which can be inhibited by the proteasome inhibitor, MG132. Our results showed 
      that treatment of human VSMCs with MCP-1 did not increase the expression of 
      MCPIP1. Treatment with MG132, however, elevated MCPIP1 protein levels through 
      stimulation of the gene transcription, but not through increasing protein 
      stability. MCPIP1 expression induced by MG132 was inhibited by alpha-amanitin 
      inhibition of gene transcription or cycloheximide inhibition of protein 
      synthesis. Our further studies showed that MCPIP1 expression induced by MG132 was 
      inhibited by the inhibitors of AKT and p38 kinase, suggesting a role of the 
      AKT-p38 pathway in MG132 effects. We also found that treatment with MG132 induces 
      apoptosis, but overexpression of MCPIP1 inhibited bromodeoxyuridine (BrdU) 
      incorporation of human VSMCs without induction of significant apoptosis. In 
      summary, MCPIP1 expression is induced by MG132 likely through activation of the 
      AKT-p38 pathway. MCPIP1 inhibits SMC proliferation without induction of 
      apoptosis. J. Cell. Physiol. 232: 122-128, 2017. (c) 2016 Wiley Periodicals, Inc.
CI  - (c) 2016 Wiley Periodicals, Inc.
FAU - Tan, Xi
AU  - Tan X
AD  - Department of Biochemistry and Molecular Biology, Smooth Muscle Research Group, 
      Libin Cardiovascular Institute of Alberta, Cumming School of Medicine, University 
      of Calgary, Calgary, Alberta, Canada.
AD  - Division of Stem Cell Regulation and Application, State Key Laboratory of Chinese 
      Medicine Powder and Medicine Innovation in Hunan (Incubation), Hunan University 
      of Chinese Medicine, Changsha, Hunan, China.
FAU - Gao, Jie
AU  - Gao J
AD  - Department of Physiology and Pharmacology, University of Calgary, Calgary, 
      Alberta, Canada.
FAU - Shi, Zhan
AU  - Shi Z
AD  - Department of Biochemistry and Molecular Biology, Smooth Muscle Research Group, 
      Libin Cardiovascular Institute of Alberta, Cumming School of Medicine, University 
      of Calgary, Calgary, Alberta, Canada.
FAU - Tai, Shi
AU  - Tai S
AD  - Department of Biochemistry and Molecular Biology, Smooth Muscle Research Group, 
      Libin Cardiovascular Institute of Alberta, Cumming School of Medicine, University 
      of Calgary, Calgary, Alberta, Canada.
FAU - Chan, Leona Loretta
AU  - Chan LL
AD  - Department of Biochemistry and Molecular Biology, Smooth Muscle Research Group, 
      Libin Cardiovascular Institute of Alberta, Cumming School of Medicine, University 
      of Calgary, Calgary, Alberta, Canada.
FAU - Yang, Yang
AU  - Yang Y
AD  - Department of Cardiology, The Second Xiangya Hospital, Central South University, 
      Changsha, Hunan, China.
FAU - Peng, Dao-Quan
AU  - Peng DQ
AD  - Department of Cardiology, The Second Xiangya Hospital, Central South University, 
      Changsha, Hunan, China.
FAU - Liao, Duan-Fang
AU  - Liao DF
AD  - Division of Stem Cell Regulation and Application, State Key Laboratory of Chinese 
      Medicine Powder and Medicine Innovation in Hunan (Incubation), Hunan University 
      of Chinese Medicine, Changsha, Hunan, China.
FAU - Jiang, Zhi-Sheng
AU  - Jiang ZS
AD  - Department of Biochemistry and Molecular Biology, Smooth Muscle Research Group, 
      Libin Cardiovascular Institute of Alberta, Cumming School of Medicine, University 
      of Calgary, Calgary, Alberta, Canada.
AD  - Institute of Cardiovascular Disease and Key Lab for Arteriosclerogy of Hunan 
      Province, University of South China, Hengyang, Hunan, China.
FAU - Chang, Ying-Zi
AU  - Chang YZ
AD  - Department of Pharmacology, A. T. Still University, Kirksville College of 
      Osteopathic Medicine, Kirksville, Missouri.
FAU - Gui, Yu
AU  - Gui Y
AD  - Department of Physiology and Pharmacology, University of Calgary, Calgary, 
      Alberta, Canada.
FAU - Zheng, Xi-Long
AU  - Zheng XL
AD  - Department of Biochemistry and Molecular Biology, Smooth Muscle Research Group, 
      Libin Cardiovascular Institute of Alberta, Cumming School of Medicine, University 
      of Calgary, Calgary, Alberta, Canada. xlzheng@ucalgary.ca.
LA  - eng
GR  - MOP-119511/CIHR/Canada
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20160414
PL  - United States
TA  - J Cell Physiol
JT  - Journal of cellular physiology
JID - 0050222
RN  - 0 (CCL2 protein, human)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Leupeptins)
RN  - 0 (Proteasome Inhibitors)
RN  - 0 (Transcription Factors)
RN  - RF1P63GW3K (benzyloxycarbonylleucyl-leucyl-leucine aldehyde)
SB  - IM
MH  - Apoptosis/*drug effects
MH  - Cell Proliferation/*drug effects
MH  - Cells, Cultured
MH  - Chemokine CCL2/*metabolism
MH  - Humans
MH  - Leupeptins/*pharmacology
MH  - Muscle, Smooth, Vascular/cytology/*drug effects
MH  - Proteasome Inhibitors/*pharmacology
MH  - Transcription Factors/metabolism
EDAT- 2016/04/02 06:00
MHDA- 2017/05/11 06:00
CRDT- 2016/04/02 06:00
PHST- 2016/01/11 00:00 [received]
PHST- 2016/03/29 00:00 [accepted]
PHST- 2016/04/02 06:00 [entrez]
PHST- 2016/04/02 06:00 [pubmed]
PHST- 2017/05/11 06:00 [medline]
AID - 10.1002/jcp.25396 [doi]
PST - ppublish
SO  - J Cell Physiol. 2017 Jan;232(1):122-8. doi: 10.1002/jcp.25396. Epub 2016 Apr 14.