PMID- 27035741
OWN - NLM
STAT- MEDLINE
DCOM- 20161226
LR  - 20181113
IS  - 1791-244X (Electronic)
IS  - 1107-3756 (Print)
IS  - 1107-3756 (Linking)
VI  - 37
IP  - 5
DP  - 2016 May
TI  - Erythronium japonicum attenuates histopathological lung abnormalities in a mouse 
      model of ovalbumin-induced asthma.
PG  - 1221-8
LID - 10.3892/ijmm.2016.2541 [doi]
AB  - Asthma is a chronic lung condition that can induce mucus hypersecretion and 
      pulmonary obstruction and may even cause death, particularly in children and 
      older individuals. Erythronium japonicum (E. japonicum) is a traditional herb 
      used in Korea and East Asian countries that has been found to exert free radical 
      scavenging activity and anti-proliferative effects in human colorectal carcinoma 
      cells. In the present study, we evaluated the anti-asthmatic effects of an 
      extract of E. japonicum in a mouse model of ovalbumin (OVA)‑induced asthma. 
      Female BALB/c mice were sensitized with an intraperitoneal injection of OVA and 
      aluminum hydroxide hydrate on days 1 and 8 and then received the following 
      treatments on days 21 to 25: i) control (no treatment), ii) sterilized tap water 
      (given orally), iii) 1 mg/kg/day dexamethasone (administered orally), iv) 60 
      mg/kg/day E. japonicum extract, and v) 600 mg/kg/day E. japonicum extract. On the 
      same days, all the mice except those in the control group were challenged 1 h 
      later with nebulized 5% OVA for 30 min. We found that treatment with E. japonicum 
      extract suppressed the OVA-induced increase in the number of white blood cells 
      and decreased the IgE level in the bronchoalveolar lavage fluid samples obtained 
      from the mice. Histopathological analysis of the lung tissues revealed that E. 
      japonicum attenuated the asthma-related morphological changes in the mouse lung 
      tissue, including the increased secretion of mucus in the bronchioles, eosinophil 
      infiltration around the bronchioles and vessels, and goblet cell and epithelial 
      cell hyperplasia. Immunohistochemical analysis revealed that treatment with E. 
      japonicum extract suppressed the OVA-induced proliferation of T helper cells 
      (CD4+) and B cells (CD19+) in the mouse lung tissue. Furthermore, treatment with 
      E. japonicum extract modulated the expression of both T helper 2 cell-related 
      factors [GATA binding protein 3 (GATA-3), tumor necrosis factor-alpha (TNF‑alpha), 
      interleukin (IL)-4, IL-5, IL-6 and IL-13], as well as that of T helper 1 
      cell-related factors [(interferon-gamma (IFN-gamma), IL-12p35 and IL-12p40]. These 
      findings suggest that E. japonicum may potentially be used as an anti-asthmatic 
      treatment.
FAU - Seo, Ji-Hye
AU  - Seo JH
AD  - Department of Oriental Medicine Materials, Dongshin University, Naju, Jeonnam 
      58245, Republic of Korea.
FAU - Bang, Mi-Ae
AU  - Bang MA
AD  - R&D Team, Jeonnam Bioindustry Foundation, Food Research Institute (JBF-FRI), 
      Naju, Jeonnam 58275, Republic of Korea.
FAU - Kim, Gyeyeop
AU  - Kim G
AD  - Department of Physical Therapy, Dongshin University, Naju, Jeonnam 58245, 
      Republic of Korea.
FAU - Cho, Seung Sik
AU  - Cho SS
AD  - College of Pharmacy, Mokpo National University, Mokpo, Jeonnam 588554, Republic 
      of Korea.
FAU - Park, Dae-Hun
AU  - Park DH
AD  - Department of Oriental Medicine Materials, Dongshin University, Naju, Jeonnam 
      58245, Republic of Korea.
LA  - eng
PT  - Journal Article
DEP - 20160328
PL  - Greece
TA  - Int J Mol Med
JT  - International journal of molecular medicine
JID - 9810955
RN  - 0 (Anti-Asthmatic Agents)
RN  - 0 (Cytokines)
RN  - 0 (GATA3 Transcription Factor)
RN  - 0 (Plant Extracts)
RN  - 0 (T-Box Domain Proteins)
RN  - 0 (T-box transcription factor TBX21)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - 9006-59-1 (Ovalbumin)
SB  - IM
MH  - Animals
MH  - Anti-Asthmatic Agents/*pharmacology
MH  - Asthma/drug therapy/*etiology/metabolism/*pathology
MH  - Bronchoalveolar Lavage Fluid
MH  - Cytokines/metabolism
MH  - Disease Models, Animal
MH  - GATA3 Transcription Factor/metabolism
MH  - Immunoglobulin E/blood/immunology
MH  - Leukocyte Count
MH  - Lung/*drug effects/*pathology
MH  - Lymphocyte Subsets/drug effects/immunology/metabolism
MH  - Mice
MH  - Ovalbumin/adverse effects
MH  - Plant Extracts/*pharmacology
MH  - Streptophyta/*chemistry
MH  - T-Box Domain Proteins/metabolism
PMC - PMC4829136
EDAT- 2016/04/02 06:00
MHDA- 2016/12/27 06:00
PMCR- 2016/03/28
CRDT- 2016/04/02 06:00
PHST- 2015/10/06 00:00 [received]
PHST- 2016/03/21 00:00 [accepted]
PHST- 2016/04/02 06:00 [entrez]
PHST- 2016/04/02 06:00 [pubmed]
PHST- 2016/12/27 06:00 [medline]
PHST- 2016/03/28 00:00 [pmc-release]
AID - ijmm-37-05-1221 [pii]
AID - 10.3892/ijmm.2016.2541 [doi]
PST - ppublish
SO  - Int J Mol Med. 2016 May;37(5):1221-8. doi: 10.3892/ijmm.2016.2541. Epub 2016 Mar 
      28.