PMID- 27036295
OWN - NLM
STAT- MEDLINE
DCOM- 20161222
LR  - 20181113
IS  - 1746-6148 (Electronic)
IS  - 1746-6148 (Linking)
VI  - 12
DP  - 2016 Mar 31
TI  - IRES-mediated translation of foot-and-mouth disease virus (FMDV) in cultured 
      cells derived from FMDV-susceptible and -insusceptible animals.
PG  - 66
LID - 10.1186/s12917-016-0694-8 [doi]
LID - 66
AB  - BACKGROUND: Foot-and-mouth disease virus (FMDV) possess a positive sense, single 
      stranded RNA genome. Internal ribosomal entry site (IRES) element exists within 
      its 5' untranslated region (5'UTR) of the viral RNA. Translation of the viral RNA 
      is initiated by internal entry of the 40S ribosome within the IRES element. This 
      process is facilitated by cellular factors known as IRES trans-acting factors 
      (ITAFs). Foot-and-mouth disease (FMD) is host-restricted disease for 
      cloven-hoofed animals such as cattle and pigs, but the factors determining the 
      host range have not been identified yet. Although, ITAFs are known to promote 
      IRES-mediated translation, these findings were confirmed only in cells derived 
      from FMDV-insusceptible animals so far. We evaluated and compared the 
      IRES-mediated translation activities among cell lines derived from four different 
      animal species using bicistronic luciferase reporter plasmid, which possesses an 
      FMDV-IRES element between Renilla and Firefly luciferase genes. Furthermore, we 
      analyzed the effect of the cellular factors on IRES-mediated translation by 
      silencing the cellular factors using siRNA in both FMDV-susceptible and 
      -insusceptible animal cells. RESULTS: Our data indicated that IRES-mediated 
      translational activity was not linked to FMDV host range. ITAF45 promoted 
      IRES-mediated translation in all cell lines, and the effects of poly-pyrimidine 
      tract binding protein (PTB) and eukaryotic initiation factor 4E-binding protein 1 
      (4E-BP1) were observed only in FMDV-susceptible cells. Thus, PTB and 4E-BP1 may 
      influence the host range of FMDV. CONCLUSIONS: IRES-mediated translation activity 
      of FMDV was not predictive of its host range. ITAF45 promoted IRES-mediated 
      translation in all cells, and the effects of PTB and 4E-BP1 were observed only in 
      FMDV-susceptible cells.
FAU - Kanda, Takehiro
AU  - Kanda T
AD  - Department of Animal Hygiene, Joint Facility of Veterinary Medicine, Kagoshima 
      University, Kagoshima, Kagoshima, Japan.
FAU - Ozawa, Makoto
AU  - Ozawa M
AD  - Department of Animal Hygiene, Joint Facility of Veterinary Medicine, Kagoshima 
      University, Kagoshima, Kagoshima, Japan.
AD  - Transboundary Animal Disease Center, Joint Facility of Veterinary Medicine, 
      Kagoshima University, Kagoshima, Kagoshima, Japan.
FAU - Tsukiyama-Kohara, Kyoko
AU  - Tsukiyama-Kohara K
AD  - Department of Animal Hygiene, Joint Facility of Veterinary Medicine, Kagoshima 
      University, Kagoshima, Kagoshima, Japan. kkohara@vet.kagoshima-u.ac.jp.
AD  - Transboundary Animal Disease Center, Joint Facility of Veterinary Medicine, 
      Kagoshima University, Kagoshima, Kagoshima, Japan. kkohara@vet.kagoshima-u.ac.jp.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20160331
PL  - England
TA  - BMC Vet Res
JT  - BMC veterinary research
JID - 101249759
RN  - 0 (Host-Derived Cellular Factors)
RN  - 0 (Internal Ribosome Entry Sites)
RN  - 0 (Phosphoproteins)
RN  - 0 (RNA, Small Interfering)
RN  - 139076-35-0 (Polypyrimidine Tract-Binding Protein)
SB  - IM
MH  - Animals
MH  - Cattle
MH  - Cell Line
MH  - Disease Susceptibility
MH  - Dogs
MH  - Foot-and-Mouth Disease Virus/genetics/*physiology
MH  - *Gene Expression Regulation, Viral
MH  - HEK293 Cells
MH  - Host Specificity/*physiology
MH  - Host-Derived Cellular Factors/metabolism
MH  - Humans
MH  - Internal Ribosome Entry Sites/genetics/*physiology
MH  - Madin Darby Canine Kidney Cells
MH  - Phosphoproteins/genetics/metabolism
MH  - Polypyrimidine Tract-Binding Protein/genetics/metabolism
MH  - RNA, Small Interfering/genetics
MH  - Swine
PMC - PMC4815274
OTO - NOTNLM
OT  - 4E-BP1
OT  - FMDV
OT  - IRES
OT  - ITAF
OT  - ITAF45
OT  - PTB
OT  - Translation
EDAT- 2016/04/03 06:00
MHDA- 2016/12/23 06:00
PMCR- 2016/03/31
CRDT- 2016/04/03 06:00
PHST- 2015/09/29 00:00 [received]
PHST- 2016/03/23 00:00 [accepted]
PHST- 2016/04/03 06:00 [entrez]
PHST- 2016/04/03 06:00 [pubmed]
PHST- 2016/12/23 06:00 [medline]
PHST- 2016/03/31 00:00 [pmc-release]
AID - 10.1186/s12917-016-0694-8 [pii]
AID - 694 [pii]
AID - 10.1186/s12917-016-0694-8 [doi]
PST - epublish
SO  - BMC Vet Res. 2016 Mar 31;12:66. doi: 10.1186/s12917-016-0694-8.