PMID- 27036746
OWN - NLM
STAT- MEDLINE
DCOM- 20160726
LR  - 20190227
IS  - 1139-6709 (Print)
IS  - 1139-6709 (Linking)
VI  - 18
IP  - 3
DP  - 2015 Sep
TI  - Dormancy in Deinococcus sp. UDEC-P1 as a survival strategy to escape from 
      deleterious effects of carbon starvation and temperature.
PG  - 189-94
LID - 10.2436/20.1501.01.249 [doi]
AB  - Dormancy is characterized by low metabolism and absence of protein synthesis and 
      cellular division enabling bacterial cells to survive under stress. The aim was 
      to determine if carbon starvation and low temperature are factors that modify the 
      proportion of dormant/active cells in Deinococcus sp. UDEC-P1. By flow cytometry, 
      RedoxSensor Green (RSG) was used to quantify metabolic activity and Propidium 
      Iodide (PI) to evaluate membrane integrity in order to determine the percentage 
      of dormant cells. Cell size and morphology were determined using scanning 
      electronic microscopy. Under carbon starvation at 30 degrees C, Deinococcus sp. UDEC-P1 
      increased its proportion of dormant cells from 0.1% to 20%, decreased the count 
      of culturable cells and average cell volume decreased 7.1 times. At 4 degrees C, however, 
      the proportion of dormant cells increased only to 6%, without a change in the 
      count of culturable cells and an average cellular volume decrease of 4.1 times 
      and 3% of the dormant cells were able to be awakened. Results indicate a greater 
      proportion of dormant Deinococcus sp. UDEC-P1 cells at 30 masculineC and it suggests that 
      carbon starvation is more deleterious condition at 30 masculineC than 4 masculineC. For this reason 
      Deinococcus sp. UDEC-P1 cells are more likely to enter into dormancy at higher 
      temperature as a strategy to survive.
CI  - Copyright(c) by the Spanish Society for Microbiology and Institute for Catalan 
      Studies.
FAU - Guerra, Matias
AU  - Guerra M
AD  - Department of Microbiology, Faculty of Biological Sciences, University of 
      Concepcion, Concepcion, Chile.
FAU - Gonzalez, Karina
AU  - Gonzalez K
AD  - Department of Microbiology, Faculty of Biological Sciences, University of 
      Concepcion, Concepcion, Chile.
FAU - Gonzalez, Carlos
AU  - Gonzalez C
AD  - Department of Microbiology, Faculty of Biological Sciences, University of 
      Concepcion, Concepcion, Chile.
FAU - Parra, Boris
AU  - Parra B
AD  - Department of Microbiology, Faculty of Biological Sciences, University of 
      Concepcion, Concepcion, Chile.
FAU - Martinez, Miguel
AU  - Martinez M
AD  - Department of Microbiology, Faculty of Biological Sciences, University of 
      Concepcion, Concepcion, Chile.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Switzerland
TA  - Int Microbiol
JT  - International microbiology : the official journal of the Spanish Society for 
      Microbiology
JID - 9816585
RN  - 7440-44-0 (Carbon)
SB  - IM
MH  - Carbon/*metabolism
MH  - Deinococcus/genetics/*growth & development/*metabolism
MH  - Ecosystem
MH  - Microbial Viability
MH  - Temperature
OTO - NOTNLM
OT  - Deinococcus
OT  - dormancy
OT  - flow cytometry
OT  - metabolism
OT  - starvation
EDAT- 2016/04/03 06:00
MHDA- 2016/07/28 06:00
CRDT- 2016/04/03 06:00
PHST- 2015/07/29 00:00 [received]
PHST- 2015/09/20 00:00 [accepted]
PHST- 2016/04/03 06:00 [entrez]
PHST- 2016/04/03 06:00 [pubmed]
PHST- 2016/07/28 06:00 [medline]
AID - 10.2436/20.1501.01.249 [doi]
PST - ppublish
SO  - Int Microbiol. 2015 Sep;18(3):189-94. doi: 10.2436/20.1501.01.249.