PMID- 27037054
OWN - NLM
STAT- MEDLINE
DCOM- 20170301
LR  - 20170302
IS  - 1879-0003 (Electronic)
IS  - 0141-8130 (Linking)
VI  - 88
DP  - 2016 Jul
TI  - Immobilization of pectinase onto Fe3O4@SiO2-NH2 and its activity and stability.
PG  - 189-95
LID - S0141-8130(16)30295-1 [pii]
LID - 10.1016/j.ijbiomac.2016.03.059 [doi]
AB  - Fe3O4 magnetic nanometer particles (MNPS) with a diameter of 30-40nm and coated 
      by ammoniated silicon dioxides were developed by sol-gel method. The X-ray 
      diffraction (XRD) pattern indicates that the Fe3O4 is wrapped in the amorphous 
      SiO2, and the Fourier-transform infrared spectroscopy (FT-IR) analysis confirmed 
      the NH2 group had been successfully introduced onto the surface of Fe3O4@SiO2 
      nanoparticles. The main factors related to the immobilization process of 
      pectinase onto the magnetic support were optimized by multi-factor orthogonal 
      experiments, and the recommended combination is that 3% glutaraldehyde (coupling 
      agent), pH=8.0, 50 degrees C, and cross-linking time 8h. Scanning electron microscope 
      (SEM) and FT-IR analyses confirmed that the pectinase had been immobilized onto 
      the surface of the amino modified nanoparticles. The storage stability and 
      reusability of the pectinase were enhanced by the immobilization that 64.4% of 
      the total starting activity was retrieved after 7 recycling batches and only 
      about 21.1% of the total activity was lost after 30-day storage. For the 
      application of the immobilized pectinase, the optimal pH and temperature were 8.5 
      and 55 degrees C, respectively.
CI  - Copyright (c) 2016 Elsevier B.V. All rights reserved.
FAU - Fang, Gang
AU  - Fang G
AD  - School of Environmental Engineering, Wuhan Textile University, Wuhan 430073, 
      China.
FAU - Chen, Honggao
AU  - Chen H
AD  - School of Environmental Engineering, Wuhan Textile University, Wuhan 430073, 
      China. Electronic address: honggao_chen@hotmail.com.
FAU - Zhang, Yunpeng
AU  - Zhang Y
AD  - School of Environmental Engineering, Wuhan Textile University, Wuhan 430073, 
      China.
FAU - Chen, Anqing
AU  - Chen A
AD  - School of Environmental Engineering, Wuhan Textile University, Wuhan 430073, 
      China.
LA  - eng
PT  - Journal Article
DEP - 20160329
PL  - Netherlands
TA  - Int J Biol Macromol
JT  - International journal of biological macromolecules
JID - 7909578
RN  - 0 (Cross-Linking Reagents)
RN  - 0 (Enzymes, Immobilized)
RN  - 0 (Magnetite Nanoparticles)
RN  - 7631-86-9 (Silicon Dioxide)
RN  - EC 3.2.1.15 (Polygalacturonase)
RN  - T3C89M417N (Glutaral)
RN  - XM0M87F357 (Ferrosoferric Oxide)
SB  - IM
MH  - Amination
MH  - Cross-Linking Reagents/chemistry
MH  - Enzyme Stability
MH  - Enzymes, Immobilized/*chemistry
MH  - Equipment Reuse
MH  - Ferrosoferric Oxide/*chemistry
MH  - Glutaral/chemistry
MH  - Hydrogen-Ion Concentration
MH  - Kinetics
MH  - Magnetite Nanoparticles/*chemistry/ultrastructure
MH  - Particle Size
MH  - Phase Transition
MH  - Polygalacturonase/*chemistry
MH  - Silicon Dioxide/*chemistry
MH  - Temperature
OTO - NOTNLM
OT  - Immobilization
OT  - Magnetic nanometer particles (MNPS)
OT  - Pectinase
OT  - Reusability
EDAT- 2016/04/03 06:00
MHDA- 2017/03/03 06:00
CRDT- 2016/04/03 06:00
PHST- 2016/01/27 00:00 [received]
PHST- 2016/03/12 00:00 [revised]
PHST- 2016/03/28 00:00 [accepted]
PHST- 2016/04/03 06:00 [entrez]
PHST- 2016/04/03 06:00 [pubmed]
PHST- 2017/03/03 06:00 [medline]
AID - S0141-8130(16)30295-1 [pii]
AID - 10.1016/j.ijbiomac.2016.03.059 [doi]
PST - ppublish
SO  - Int J Biol Macromol. 2016 Jul;88:189-95. doi: 10.1016/j.ijbiomac.2016.03.059. 
      Epub 2016 Mar 29.