PMID- 27038826
OWN - NLM
STAT- MEDLINE
DCOM- 20170203
LR  - 20170203
IS  - 1432-8798 (Electronic)
IS  - 0304-8608 (Linking)
VI  - 161
IP  - 7
DP  - 2016 Jul
TI  - A single R36Q mutation in the matrix protein of pigeon paramyxovirus type 1 
      reduces virus replication and shedding in pigeons.
PG  - 1949-55
LID - 10.1007/s00705-016-2847-2 [doi]
AB  - Pigeon paramyxovirus type 1 (PPMV-1) is considered an antigenic and variant of 
      avian paramyxovirus type 1 (APMV-1) that has adapted to pigeons as hosts. 
      However, how this host-specific adaption of PPMV-1 is related to its biological 
      characteristics is unknown. In this study, seven unique amino acids in PPMV-1 
      that are not present in other APMV-1 strains (n = 39 versus n = 106) were 
      identified. R36 of the M protein was found to be not only a unique amino acid but 
      also a positive-selection site. To investigate the role of R36 in host 
      adaptation, a recombinant PPMV-1 with R36Q mutation was constructed. Our results 
      indicated that the an R36Q mutation significantly attenuates pathogenicity in 
      chickens, viral growth in both chicken embryo fibroblasts (CEFs) and pigeon 
      embryo fibroblasts (PEFs), and virus replication and shedding in pigeons in 
      comparison with the wild-type virus, suggesting that R36 is a key residue that 
      evolved during the adaptation of PPMV-1 in pigeons.
FAU - Xu, Haixu
AU  - Xu H
AD  - Animal Infectious Disease Laboratory, College of Veterinary Medicine, Yangzhou 
      University, 12 East Wenhui Road, Yangzhou, 225009, Jiangsu, China.
AD  - Jiangsu Co-innovation Center for Prevention and Control of Important Animal 
      Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou, 225009, China.
FAU - Song, Qingqing
AU  - Song Q
AD  - Animal Infectious Disease Laboratory, College of Veterinary Medicine, Yangzhou 
      University, 12 East Wenhui Road, Yangzhou, 225009, Jiangsu, China.
AD  - Jiangsu Co-innovation Center for Prevention and Control of Important Animal 
      Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou, 225009, China.
FAU - Zhu, Jie
AU  - Zhu J
AD  - Shandong Binzhou Wohua Biological Engineering Co., Ltd., Binzhou, 256600, China.
FAU - Liu, Jiajia
AU  - Liu J
AD  - Animal Infectious Disease Laboratory, College of Veterinary Medicine, Yangzhou 
      University, 12 East Wenhui Road, Yangzhou, 225009, Jiangsu, China.
AD  - Jiangsu Co-innovation Center for Prevention and Control of Important Animal 
      Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou, 225009, China.
FAU - Cheng, Xin
AU  - Cheng X
AD  - Animal Infectious Disease Laboratory, College of Veterinary Medicine, Yangzhou 
      University, 12 East Wenhui Road, Yangzhou, 225009, Jiangsu, China.
AD  - Jiangsu Co-innovation Center for Prevention and Control of Important Animal 
      Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou, 225009, China.
FAU - Hu, Shunlin
AU  - Hu S
AD  - Animal Infectious Disease Laboratory, College of Veterinary Medicine, Yangzhou 
      University, 12 East Wenhui Road, Yangzhou, 225009, Jiangsu, China.
AD  - Jiangsu Co-innovation Center for Prevention and Control of Important Animal 
      Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou, 225009, China.
FAU - Wu, Shuang
AU  - Wu S
AD  - Jiangsu Agri-animal Husbandry Vocational College, Taizhou, 225300, China.
FAU - Wang, Xiaoquan
AU  - Wang X
AD  - Animal Infectious Disease Laboratory, College of Veterinary Medicine, Yangzhou 
      University, 12 East Wenhui Road, Yangzhou, 225009, Jiangsu, China.
AD  - Jiangsu Co-innovation Center for Prevention and Control of Important Animal 
      Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou, 225009, China.
FAU - Liu, Xiaowen
AU  - Liu X
AD  - Animal Infectious Disease Laboratory, College of Veterinary Medicine, Yangzhou 
      University, 12 East Wenhui Road, Yangzhou, 225009, Jiangsu, China.
AD  - Jiangsu Co-innovation Center for Prevention and Control of Important Animal 
      Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou, 225009, China.
FAU - Liu, Xiufan
AU  - Liu X
AD  - Animal Infectious Disease Laboratory, College of Veterinary Medicine, Yangzhou 
      University, 12 East Wenhui Road, Yangzhou, 225009, Jiangsu, China. 
      xfliu@yzu.edu.cn.
AD  - Jiangsu Co-innovation Center for Prevention and Control of Important Animal 
      Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou, 225009, China. 
      xfliu@yzu.edu.cn.
LA  - eng
PT  - Journal Article
DEP - 20160402
PL  - Austria
TA  - Arch Virol
JT  - Archives of virology
JID - 7506870
RN  - 0 (Viral Matrix Proteins)
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - Columbidae/*virology
MH  - Molecular Sequence Data
MH  - *Mutation, Missense
MH  - Newcastle Disease/*virology
MH  - Newcastle disease virus/genetics/*physiology
MH  - Viral Matrix Proteins/*genetics/metabolism
MH  - *Virus Replication
MH  - *Virus Shedding
OTO - NOTNLM
OT  - Host-specific adaptation
OT  - Matrix protein
OT  - Pigeon paramyxovirus type 1
OT  - R36
OT  - Unique amino acid
EDAT- 2016/04/04 06:00
MHDA- 2017/02/06 06:00
CRDT- 2016/04/04 06:00
PHST- 2015/11/19 00:00 [received]
PHST- 2016/03/24 00:00 [accepted]
PHST- 2016/04/04 06:00 [entrez]
PHST- 2016/04/04 06:00 [pubmed]
PHST- 2017/02/06 06:00 [medline]
AID - 10.1007/s00705-016-2847-2 [pii]
AID - 10.1007/s00705-016-2847-2 [doi]
PST - ppublish
SO  - Arch Virol. 2016 Jul;161(7):1949-55. doi: 10.1007/s00705-016-2847-2. Epub 2016 
      Apr 2.