PMID- 27040221 OWN - NLM STAT- MEDLINE DCOM- 20161228 LR - 20161230 IS - 1873-0191 (Electronic) IS - 0928-4931 (Linking) VI - 63 DP - 2016 Jun TI - Preparation, characterization and properties of nano-hydroxyapatite/polypropylene carbonate biocomposite. PG - 285-91 LID - S0928-4931(16)30146-1 [pii] LID - 10.1016/j.msec.2016.02.054 [doi] AB - The combination of nano-hydroxyapatite (n-HA) and polypropylene carbonate (PPC) was used to make a composite materials by a coprecipitation method. The physical and chemical properties of the composite were tested. Scanning electron microscope (SEM) observation indicated that the biomimetic n-HA crystals were uniformly distributed in the polymer matrix. As the n-HA content increased in the composite, the fracture mechanism of the composites changes from gliding fracture to gliding and brittle fracture. Furthermore, the chemical interaction between inorganic n-HA and polypropylene carbonate was also investigated and discussed in detail. The hydrogen bonds might be formed between -OH/CO3(2-) of n-HA crystal and the ester group (-COO-) of PPC. The tensile strength of n-HA/PPC (40/60) was similar to that of the cancellous bone, and reached ca 58 MPa. The osteoblasts were cultured for up to 7 days, and then the adhesion and proliferation of osteoblasts were measured by Methyl thiazolyl tetrazolium (MTT) colorimetry assay and SEM. The cells proliferated, grew normally in fusiform shape and well attached. The in vitro test confirmed that the n-HA/PPC composites were biocompatible and showed undetectable negative effect on osteoblasts. In vivo implantation of the composite in New Zealand white rabbits was performed. It can stimulate the growth of a new bone, and at the same time the material begins to degrade. These results suggested that the composite may be suitable for the reparation or replacement of bone defects and possessed the potential for clinical applications. CI - Copyright (c) 2016 Elsevier B.V. All rights reserved. FAU - Liao, Jianguo AU - Liao J AD - School of Materials Science and Engineering, Henan Polytechnic University, Jiaozuo 454000, China. Electronic address: liaojianguo10@hpu.edu.cn. FAU - Li, Yanqun AU - Li Y AD - School of Materials Science and Engineering, Henan Polytechnic University, Jiaozuo 454000, China. FAU - Zou, Qin AU - Zou Q AD - Analytical & Testing Center, Sichuan University, Chengdu 610064, China. FAU - Duan, Xingze AU - Duan X AD - School of Materials Science and Engineering, Henan Polytechnic University, Jiaozuo 454000, China. FAU - Yang, Zhengpeng AU - Yang Z AD - School of Materials Science and Engineering, Henan Polytechnic University, Jiaozuo 454000, China. FAU - Xie, Yufen AU - Xie Y AD - School of Materials Science and Engineering, Henan Polytechnic University, Jiaozuo 454000, China. FAU - Liu, Haohuai AU - Liu H AD - School of Chemistry and Chemical Engineering, Analytical and Testing Center, Guangzhou University, Guangzhou 510006, China. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20160222 PL - Netherlands TA - Mater Sci Eng C Mater Biol Appl JT - Materials science & engineering. C, Materials for biological applications JID - 101484109 RN - 0 (Biocompatible Materials) RN - 0 (Bone Substitutes) RN - 0 (Polypropylenes) RN - 50862-75-4 (polypropylene carbonate) RN - 91D9GV0Z28 (Durapatite) SB - IM MH - Animals MH - Biocompatible Materials/*chemistry/pharmacology MH - Bone Substitutes/chemistry/pharmacology MH - Cell Line MH - Cell Survival/drug effects MH - Durapatite/*chemistry MH - Femur/pathology MH - Humans MH - Microscopy, Electron, Scanning MH - Polypropylenes/*chemistry MH - Rabbits MH - Spectroscopy, Fourier Transform Infrared MH - Spectrum Analysis, Raman MH - Tensile Strength MH - X-Ray Diffraction OTO - NOTNLM OT - Biocompatibility OT - Bone substitute OT - Composite OT - Nano-hydroxyapatite OT - Polypropylene carbonate EDAT- 2016/04/05 06:00 MHDA- 2016/12/29 06:00 CRDT- 2016/04/05 06:00 PHST- 2015/09/24 00:00 [received] PHST- 2016/01/21 00:00 [revised] PHST- 2016/02/19 00:00 [accepted] PHST- 2016/04/05 06:00 [entrez] PHST- 2016/04/05 06:00 [pubmed] PHST- 2016/12/29 06:00 [medline] AID - S0928-4931(16)30146-1 [pii] AID - 10.1016/j.msec.2016.02.054 [doi] PST - ppublish SO - Mater Sci Eng C Mater Biol Appl. 2016 Jun;63:285-91. doi: 10.1016/j.msec.2016.02.054. Epub 2016 Feb 22.