PMID- 27040676
OWN - NLM
STAT- MEDLINE
DCOM- 20180129
LR  - 20181203
IS  - 1432-0827 (Electronic)
IS  - 0171-967X (Linking)
VI  - 99
IP  - 2
DP  - 2016 Aug
TI  - Effect of miR-26a-5p on the Wnt/Ca(2+) Pathway and Osteogenic Differentiation of 
      Mouse Adipose-Derived Mesenchymal Stem Cells.
PG  - 174-86
LID - 10.1007/s00223-016-0137-3 [doi]
AB  - Elucidation of the molecular mechanisms that regulate the differentiation of 
      adipose-derived mesenchymal stem cells into osteogenic cells may lead to new 
      methods for bone tissue engineering. We examined the role of miR-26a-5p in the 
      regulation of osteogenic differentiation of mouse adipose-derived mesenchymal 
      stem cells (mADSCs) by using mimics and inhibitors of this microRNA. Our results 
      showed that over-expression of miR-26a-5p inhibited osteogenesis and that 
      suppression of endogenous miR-26a-5p promoted osteogenesis. Four bioinformatics 
      algorithms indicated that the 3'UTR of Wnt5a was a potential target of 
      miR-26a-5p. We confirmed this prediction by use of dual-luciferase reporter assay 
      and GFP/RFP assay. We also examined the molecular mechanisms by which miR-26a-5p 
      regulates osteogenesis. Fura-2AM and Western blot assays after transfection 
      indicated that miR-26a-5p repressed WNT5A, inhibited calcium flux and protein 
      kinase C, and suppressed osteogenic differentiation of mADSCs. By contrast, 
      miR-26a-5p inhibition activated these signal proteins and promoted osteogenic 
      differentiation. Taken together, our results suggest that up-regulation of 
      miR-26a-5p inhibits osteogenic differentiation of mADSCs by directly targeting 
      the 3'UTR of Wnt5a, thereby down-regulating the Wnt/Ca(2+) signaling pathway.
FAU - Li, Shasha
AU  - Li S
AD  - The State Key Laboratory of Oral Diseases, Sichuan University, Chengdu, 610041, 
      People's Republic of China.
FAU - Hu, Chen
AU  - Hu C
AD  - Department of Oral and Maxillofacial Surgery, General Hospital of Ningxia Medical 
      University, Yinchuan, 750004, People's Republic of China.
FAU - Li, Jianwei
AU  - Li J
AD  - Department of Oral and Maxillofacial Surgery, Binzhou Medical University 
      Hospital, Binzhou, 256603, People's Republic of China.
FAU - Liu, Lei
AU  - Liu L
AD  - The State Key Laboratory of Oral Diseases, Sichuan University, Chengdu, 610041, 
      People's Republic of China.
FAU - Jing, Wei
AU  - Jing W
AD  - The State Key Laboratory of Oral Diseases, Sichuan University, Chengdu, 610041, 
      People's Republic of China.
FAU - Tang, Wei
AU  - Tang W
AD  - The State Key Laboratory of Oral Diseases, Sichuan University, Chengdu, 610041, 
      People's Republic of China.
FAU - Tian, Weidong
AU  - Tian W
AD  - The State Key Laboratory of Oral Diseases, Sichuan University, Chengdu, 610041, 
      People's Republic of China.
FAU - Long, Jie
AU  - Long J
AD  - The State Key Laboratory of Oral Diseases, Sichuan University, Chengdu, 610041, 
      People's Republic of China. dr.jielong@hotmail.com.
AD  - Department of Oral and Maxillofacial Surgery, West China College of Stomatology, 
      Sichuan University, Chengdu, 610041, People's Republic of China. 
      dr.jielong@hotmail.com.
LA  - eng
PT  - Journal Article
DEP - 20160404
PL  - United States
TA  - Calcif Tissue Int
JT  - Calcified tissue international
JID - 7905481
RN  - 0 (MicroRNAs)
RN  - 0 (Mirn26 microRNA, mouse)
RN  - 0 (Wnt Proteins)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Adipose Tissue/cytology/metabolism
MH  - Adiposity/physiology
MH  - Animals
MH  - Calcium/metabolism
MH  - Cell Differentiation/*genetics
MH  - Gene Expression Regulation/genetics
MH  - Male
MH  - Mesenchymal Stem Cells/*cytology/*metabolism
MH  - Mice, Inbred C57BL
MH  - MicroRNAs/*genetics
MH  - Osteoblasts/*cytology/metabolism
MH  - Osteogenesis/*genetics
MH  - Wnt Proteins/metabolism
MH  - Wnt Signaling Pathway/genetics
OTO - NOTNLM
OT  - Adipose-derived mesenchymal stem cell (ADSC)
OT  - MicroRNA-26a-5p
OT  - Non-canonical Wnt pathway
OT  - Osteogenic differentiation
OT  - WNT5A
EDAT- 2016/04/05 06:00
MHDA- 2018/01/30 06:00
CRDT- 2016/04/05 06:00
PHST- 2016/02/02 00:00 [received]
PHST- 2016/03/24 00:00 [accepted]
PHST- 2016/04/05 06:00 [entrez]
PHST- 2016/04/05 06:00 [pubmed]
PHST- 2018/01/30 06:00 [medline]
AID - 10.1007/s00223-016-0137-3 [pii]
AID - 10.1007/s00223-016-0137-3 [doi]
PST - ppublish
SO  - Calcif Tissue Int. 2016 Aug;99(2):174-86. doi: 10.1007/s00223-016-0137-3. Epub 
      2016 Apr 4.