PMID- 27042289
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20160404
LR  - 20181113
IS  - 2042-6410 (Print)
IS  - 2042-6410 (Electronic)
IS  - 2042-6410 (Linking)
VI  - 7
DP  - 2016
TI  - Expression profile of androgen-modulated microRNAs in the fetal murine lung.
PG  - 20
LID - 10.1186/s13293-016-0072-z [doi]
LID - 20
AB  - BACKGROUND: Androgens are known to delay lung development. As a consequence, the 
      incidence and morbidity of respiratory distress syndrome of the neonate are 
      higher for male than for female premature infants. We previously reported that 
      many genes were expressed with a sex difference in the mouse developing lung and 
      that several genes were under the control of androgens in the male fetal lung. 
      microRNAs are small non-coding RNAs known to negatively regulate the expression 
      of specific genes. In this study, we examined whether murine miRNAs are under the 
      control of androgens in the male developing lung. METHODS: Expression profiling 
      of microRNAs was performed by microarrays using RNA extracted from male fetal 
      lungs isolated on gestational day (GD) 17.0 and GD 18.0 after daily injection of 
      pregnant mice from GD 10.0 with the antiandrogen flutamide or vehicle only. To 
      identify putative miRNA target genes, the data obtained here were combined with 
      gene profiling data reported previously using the same RNA preparations. qPCR was 
      used to confirm microarray data with fetal lungs from other litters than those 
      used in microarrays. RESULTS: Flutamide induced downregulation and upregulation 
      of several miRNAs on GD 17.0 and GD 18.0. Of the 43 mature miRNAs modulated by 
      flutamide on GD 17.0, 60 % were downregulated, whereas this proportion was only 
      of 34 % for the 35 mature miRNAs modulated on GD 18.0. For 29 and 26 
      flutamide-responsive miRNAs, we found a corresponding target inversely regulated 
      by androgens on GD 17.0 and 18.0, respectively. The androgen-regulated target 
      genes were involved in several biological processes (lipid metabolism, cell 
      proliferation, and lung development) and molecular functions, mainly 
      transcription factor binding. CONCLUSIONS: Regulation of male lung development 
      involves several miRNAs that are under androgen modulation in vivo.
FAU - Bouhaddioui, Wafae
AU  - Bouhaddioui W
AD  - Reproduction, Mother and Youth Health, Centre de Recherche du CHU de Quebec, 2705 
      Laurier Boulevard, Rm T-3-67, Quebec City, Quebec Canada ; Centre de Recherche en 
      Biologie de la Reproduction (CRBR), Faculte de Medecine, Universite Laval, Quebec 
      City, Quebec Canada.
FAU - Provost, Pierre R
AU  - Provost PR
AD  - Reproduction, Mother and Youth Health, Centre de Recherche du CHU de Quebec, 2705 
      Laurier Boulevard, Rm T-3-67, Quebec City, Quebec Canada ; Department of 
      Obstetrics/Gynecology and Reproduction, Faculty of Medicine, Universite Laval, 
      Quebec City, Quebec Canada ; Centre de Recherche en Biologie de la Reproduction 
      (CRBR), Faculte de Medecine, Universite Laval, Quebec City, Quebec Canada.
FAU - Tremblay, Yves
AU  - Tremblay Y
AD  - Reproduction, Mother and Youth Health, Centre de Recherche du CHU de Quebec, 2705 
      Laurier Boulevard, Rm T-3-67, Quebec City, Quebec Canada ; Department of 
      Obstetrics/Gynecology and Reproduction, Faculty of Medicine, Universite Laval, 
      Quebec City, Quebec Canada ; Centre de Recherche en Biologie de la Reproduction 
      (CRBR), Faculte de Medecine, Universite Laval, Quebec City, Quebec Canada.
LA  - eng
PT  - Journal Article
DEP - 20160401
PL  - England
TA  - Biol Sex Differ
JT  - Biology of sex differences
JID - 101548963
PMC - PMC4818395
OTO - NOTNLM
OT  - Androgens
OT  - Lung development
OT  - Preterm birth
OT  - Respiratory distress syndrome
OT  - Sex differences
OT  - Surfactant
OT  - microRNA
EDAT- 2016/04/05 06:00
MHDA- 2016/04/05 06:01
PMCR- 2016/04/01
CRDT- 2016/04/05 06:00
PHST- 2015/11/23 00:00 [received]
PHST- 2016/03/21 00:00 [accepted]
PHST- 2016/04/05 06:00 [entrez]
PHST- 2016/04/05 06:00 [pubmed]
PHST- 2016/04/05 06:01 [medline]
PHST- 2016/04/01 00:00 [pmc-release]
AID - 72 [pii]
AID - 10.1186/s13293-016-0072-z [doi]
PST - epublish
SO  - Biol Sex Differ. 2016 Apr 1;7:20. doi: 10.1186/s13293-016-0072-z. eCollection 
      2016.