PMID- 27043532
OWN - NLM
STAT- MEDLINE
DCOM- 20161213
LR  - 20181113
IS  - 1422-0067 (Electronic)
IS  - 1422-0067 (Linking)
VI  - 17
IP  - 4
DP  - 2016 Mar 29
TI  - Multiplex Immunoassay of Plasma Cytokine Levels in Men with Alcoholism and the 
      Relationship to Psychiatric Assessments.
PG  - 472
LID - 10.3390/ijms17040472 [doi]
LID - 472
AB  - Chronic alcohol use alters adaptive immunity and cytokine activity influencing 
      immunological and hormone responses, inflammation, and wound healing. Brain 
      cytokine disturbances may impact neurological function, mood, cognition and 
      traits related to alcoholism including impulsiveness. We examined the 
      relationship between plasma cytokine levels and self-rated psychiatric symptoms 
      in 40 adult males (mean age 51 +/- 6 years; range 33-58 years) with current alcohol 
      dependence and 30 control males (mean age 48 +/- 6 years; range 40-58 years) with 
      no history of alcoholism using multiplex sandwich immunoassays with the Luminex 
      magnetic-bead based platform. Log-transformed cytokine levels were analyzed for 
      their relationship with the Symptom Checklist-90R (SCL-90R), Barratt Impulsivity 
      Scales (BIS) and Alcoholism Severity Scale (ASS). Inflammatory cytokines 
      (interferon gamma-induced protein-10 (IP-10); monocyte chemoattractant protein-1 
      (MCP1); regulated on activation, normal T cell expressed and secreted (RANTES)) 
      were significantly elevated in alcoholism compared to controls while bone 
      marrow-derived hematopoietic cytokines and chemokines (granulocyte-colony 
      stimulating factor (GCSF); soluble CD40 ligand (sCD40L); growth-related oncogene 
      (GRO)) were significantly reduced. GRO and RANTES levels were positively 
      correlated with BIS scales; and macrophage-derived chemokine (MDC) levels were 
      positively correlated with SCL-90R scale scores (p < 0.05). Elevated inflammatory 
      mediators in alcoholism may influence brain function leading to increased 
      impulsiveness and/or phobia. The novel association between RANTES and GRO and 
      impulsivity phenotype in alcoholism should be further investigated in alcoholism 
      and psychiatric conditions with core impulsivity and anxiety phenotypes lending 
      support for therapeutic intervention.
FAU - Manzardo, Ann M
AU  - Manzardo AM
AD  - Departments of Psychiatry &amp; Behavioral Sciences and Pediatrics, University of 
      Kansas Medical Center, Kansas City, MO 64101, USA. amanzardo@kumc.edu.
FAU - Poje, Albert B
AU  - Poje AB
AD  - Departments of Psychiatry &amp; Behavioral Sciences and Pediatrics, University of 
      Kansas Medical Center, Kansas City, MO 64101, USA. apoje@kumc.edu.
FAU - Penick, Elizabeth C
AU  - Penick EC
AD  - Departments of Psychiatry &amp; Behavioral Sciences and Pediatrics, University of 
      Kansas Medical Center, Kansas City, MO 64101, USA. epenick@kumc.edu.
FAU - Butler, Merlin G
AU  - Butler MG
AD  - Departments of Psychiatry &amp; Behavioral Sciences and Pediatrics, University of 
      Kansas Medical Center, Kansas City, MO 64101, USA. mbutler4@kumc.edu.
LA  - eng
GR  - UL1TR000001/TR/NCATS NIH HHS/United States
GR  - HD02528/HD/NICHD NIH HHS/United States
GR  - P30 GM103326/GM/NIGMS NIH HHS/United States
GR  - UL1 RR033179/RR/NCRR NIH HHS/United States
GR  - UL1 TR000001/TR/NCATS NIH HHS/United States
GR  - M01 RR023940/RR/NCRR NIH HHS/United States
GR  - MO1RR023940/RR/NCRR NIH HHS/United States
GR  - UL1RR033179/RR/NCRR NIH HHS/United States
GR  - P30 HD002528/HD/NICHD NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20160329
PL  - Switzerland
TA  - Int J Mol Sci
JT  - International journal of molecular sciences
JID - 101092791
RN  - 0 (CCL2 protein, human)
RN  - 0 (CXCL10 protein, human)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Chemokine CCL5)
RN  - 0 (Chemokine CXCL10)
RN  - 0 (Chemokines)
RN  - 0 (Cytokines)
SB  - IM
MH  - Adult
MH  - Alcoholism/blood/*diagnosis
MH  - Chemokine CCL2/blood
MH  - Chemokine CCL5/blood
MH  - Chemokine CXCL10/blood
MH  - Chemokines/blood
MH  - Cytokines/*blood
MH  - Humans
MH  - Immunoassay
MH  - Linear Models
MH  - Male
MH  - Middle Aged
PMC - PMC4848928
OTO - NOTNLM
OT  - GRO
OT  - MDC
OT  - RANTES
OT  - anxiety
OT  - cytokines
OT  - impulsivity
OT  - male alcoholism
EDAT- 2016/04/05 06:00
MHDA- 2016/12/15 06:00
PMCR- 2016/04/01
CRDT- 2016/04/05 06:00
PHST- 2016/02/10 00:00 [received]
PHST- 2016/03/22 00:00 [revised]
PHST- 2016/03/24 00:00 [accepted]
PHST- 2016/04/05 06:00 [entrez]
PHST- 2016/04/05 06:00 [pubmed]
PHST- 2016/12/15 06:00 [medline]
PHST- 2016/04/01 00:00 [pmc-release]
AID - ijms17040472 [pii]
AID - ijms-17-00472 [pii]
AID - 10.3390/ijms17040472 [doi]
PST - epublish
SO  - Int J Mol Sci. 2016 Mar 29;17(4):472. doi: 10.3390/ijms17040472.