PMID- 27046221
OWN - NLM
STAT- MEDLINE
DCOM- 20160815
LR  - 20190219
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 11
IP  - 4
DP  - 2016
TI  - Geniposide Protects Primary Cortical Neurons against Oligomeric Abeta1-42-Induced 
      Neurotoxicity through a Mitochondrial Pathway.
PG  - e0152551
LID - 10.1371/journal.pone.0152551 [doi]
LID - e0152551
AB  - Mitochondrial dysfunction plays a key role in the progression of Alzheimer's 
      disease (AD). The accumulation of amyloid-beta peptide (Abeta) in the brains of AD 
      patients is thought to be closely related to neuronal mitochondrial dysfunction 
      and oxidative stress. Therefore, protecting mitochondria from Abeta-induced 
      neurotoxicity is an effective strategy for AD therapeutics. In a previous study, 
      we found that geniposide, a pharmacologically active compound purified from 
      gardenia fruit, has protective effects on oxidative stress and mitochondrial 
      dysfunction in AD transgenic mouse models. However, whether geniposide has a 
      protective effect on Abeta-induced neuronal dysfunction remains unknown. In the 
      present study, we demonstrate that geniposide protects cultured primary cortical 
      neurons from Abeta-mediated mitochondrial dysfunction by recovering ATP generation, 
      mitochondrial membrane potential (MMP), and cytochrome c oxidase (CcO) and 
      caspase 3/9 activity; by reducing ROS production and cytochrome c leakage; as 
      well as by inhibiting apoptosis. These findings suggest that geniposide may 
      attenuate Abeta-induced neuronal injury by inhibiting mitochondrial dysfunction and 
      oxidative stress.
FAU - Zhao, Chunhui
AU  - Zhao C
AD  - Beijing Area Major Laboratory of Protection and Utilization of Traditional 
      Chinese Medicine, Beijing Normal University, Beijing, China.
AD  - College of Resources Science Technology, Beijing Normal University, Beijing, 
      China.
AD  - Engineering Research Center of Natural Medicine, Ministry of Education, Beijing 
      Normal University, Beijing, China.
FAU - Lv, Cui
AU  - Lv C
AD  - Beijing Area Major Laboratory of Protection and Utilization of Traditional 
      Chinese Medicine, Beijing Normal University, Beijing, China.
AD  - Laboratory of Immunology for Environment and Health, Shandong Analysis and Test 
      Center, Shandong Academy of science, Jinan, China.
AD  - College of Resources Science Technology, Beijing Normal University, Beijing, 
      China.
FAU - Li, Hang
AU  - Li H
AD  - Beijing Area Major Laboratory of Protection and Utilization of Traditional 
      Chinese Medicine, Beijing Normal University, Beijing, China.
AD  - College of Resources Science Technology, Beijing Normal University, Beijing, 
      China.
AD  - Engineering Research Center of Natural Medicine, Ministry of Education, Beijing 
      Normal University, Beijing, China.
FAU - Du, Shijing
AU  - Du S
AD  - Beijing Area Major Laboratory of Protection and Utilization of Traditional 
      Chinese Medicine, Beijing Normal University, Beijing, China.
AD  - College of Resources Science Technology, Beijing Normal University, Beijing, 
      China.
AD  - Engineering Research Center of Natural Medicine, Ministry of Education, Beijing 
      Normal University, Beijing, China.
FAU - Liu, Xiaoli
AU  - Liu X
AD  - Engineering Research Center of Natural Medicine, Ministry of Education, Beijing 
      Normal University, Beijing, China.
AD  - Engineering Research Center of Sanqi Biotechnology and Pharmaceutical, Yun Nan 
      Province, Kunming, China.
FAU - Li, Zhi
AU  - Li Z
AD  - Engineering Research Center of Natural Medicine, Ministry of Education, Beijing 
      Normal University, Beijing, China.
FAU - Xin, Wenfeng
AU  - Xin W
AD  - Engineering Research Center of Natural Medicine, Ministry of Education, Beijing 
      Normal University, Beijing, China.
AD  - Engineering Research Center of Sanqi Biotechnology and Pharmaceutical, Yun Nan 
      Province, Kunming, China.
FAU - Zhang, Wensheng
AU  - Zhang W
AD  - Beijing Area Major Laboratory of Protection and Utilization of Traditional 
      Chinese Medicine, Beijing Normal University, Beijing, China.
AD  - College of Resources Science Technology, Beijing Normal University, Beijing, 
      China.
AD  - Engineering Research Center of Natural Medicine, Ministry of Education, Beijing 
      Normal University, Beijing, China.
AD  - Engineering Research Center of Sanqi Biotechnology and Pharmaceutical, Yun Nan 
      Province, Kunming, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20160405
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (Amyloid beta-Peptides)
RN  - 0 (Biopolymers)
RN  - 0 (Iridoids)
RN  - 0 (Peptide Fragments)
RN  - 0 (Reactive Oxygen Species)
RN  - 0 (amyloid beta-protein (1-42))
RN  - 145295QLXY (geniposide)
SB  - IM
MH  - Amyloid beta-Peptides/*metabolism
MH  - Animals
MH  - Apoptosis/drug effects
MH  - Biopolymers/chemistry/*pharmacology
MH  - Cerebral Cortex/cytology/*drug effects
MH  - Female
MH  - Iridoids/*pharmacology
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Transgenic
MH  - Mitochondria/*metabolism
MH  - Neurons/*drug effects
MH  - Peptide Fragments/*metabolism
MH  - Reactive Oxygen Species/metabolism
PMC - PMC4821580
COIS- Competing Interests: The authors have declared that no competing interests exist.
EDAT- 2016/04/06 06:00
MHDA- 2016/08/16 06:00
PMCR- 2016/04/05
CRDT- 2016/04/06 06:00
PHST- 2015/11/14 00:00 [received]
PHST- 2016/03/16 00:00 [accepted]
PHST- 2016/04/06 06:00 [entrez]
PHST- 2016/04/06 06:00 [pubmed]
PHST- 2016/08/16 06:00 [medline]
PHST- 2016/04/05 00:00 [pmc-release]
AID - PONE-D-15-48943 [pii]
AID - 10.1371/journal.pone.0152551 [doi]
PST - epublish
SO  - PLoS One. 2016 Apr 5;11(4):e0152551. doi: 10.1371/journal.pone.0152551. 
      eCollection 2016.