PMID- 27047168
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20160406
LR  - 20200930
IS  - 0972-8988 (Print)
IS  - 2231-0916 (Electronic)
IS  - 0972-8988 (Linking)
VI  - 8
IP  - 7
DP  - 2015 Jul
TI  - Molecular identification and genetic diversity of open reading frame 7 field 
      isolated porcine reproductive and respiratory syndrome in North Sumatera, 
      Indonesia, in the period of 2008-2014.
PG  - 875-80
LID - 10.14202/vetworld.2015.875-880 [doi]
AB  - AIM: Molecular identification and genetic diversity of open reading frame 7 
      (ORF7) of field isolated porcine reproductive and respiratory syndrome virus 
      (PRRSV) in North Sumatera, Indonesia, in the period of 2008-2014. MATERIALS AND 
      METHODS: A total of 47 PRRSV samples were collected from the death case of pigs. 
      The samples were collected from different districts in the period of 2008-2014 
      from North Sumatera province. Two pairs of primer were designed to amplify ORF7 
      of Type 1 and 2 PRRSV based on the sequence of reference viruses VR2332 and 
      Lelystad. Viral RNAs were extracted from samples using PureLink micro-to-Midi 
      total RNA purification system (Invitrogen). To amplify the ORF7 of PRRSV, the 
      synthesis cDNA and DNA amplification were performed by reverse transcription 
      polymerase chain reaction (RT-PCR) and nested PCR method. Then the DNA sequencing 
      of PCR products and phylogenetic analysis were accomplished by molecular 
      evolutionary genetics analysis version 6.0 software program. RESULTS: RT-: PCR 
      and nested PCR used in this study had successfully detected of 18 samples 
      positive PRRS virus with the amplification products at 703bp and 508bp, 
      respectively. Sequencing of the ORF7 shows that 18 PRRS viruses isolated from 
      North Sumatera belonged to North American (NA). JXA1 Like and classic NA type 
      viruses. Several mutations were detected, particularly in the area of nuclear 
      localization signal (NLS1) and in NLS2. In the local viruses, which were related 
      closed to JXA1 virus; there are two differences in amino acids in position 12 and 
      43 of ORF7. Our tested viruses showed that the amino acid positions 12 and 43 are 
      Asparagine and Arginine, while the reference virus (VR2332, Lelystad, and JXA1) 
      occupied both by Lysine. Based on differences in two amino acids at position 12 
      and 43 showed that viruses from North Sumatera has its own uniqueness and related 
      closed to highly pathogenic PRRS (HP-PRRS) virus (JXA1). CONCLUSION: The results 
      demonstrated that North Sumatera type PRRS virus has caused PRRS outbreaks in pig 
      in North Sumatera between 2008 and 2014. The JAX1 like viruses had unique amino 
      acid residue in position 12 and 43 of asparagine and lysine, and these were 
      genetic determinants of North Sumatera viruses compared to other PRRS viruses.
FAU - Faisal, Faisal
AU  - Faisal F
AD  - Department of Veterinary Science, Faculty of Veterinary Medicine, Gadjah Mada 
      University, Yogyakarta, Indonesia; Department of Molecular Biology, Animal 
      Disease Investigation Centre of Medan, North Sumatera, Indonesia.
FAU - Widayanti, Rini
AU  - Widayanti R
AD  - Department of Biochemistry, Faculty of Veterinary Medicine, Gadjah Mada 
      University, Yogyakarta, Indonesia.
FAU - Haryanto, Aris
AU  - Haryanto A
AD  - Department of Biochemistry, Faculty of Veterinary Medicine, Gadjah Mada 
      University, Yogyakarta, Indonesia.
FAU - Tabu, Charles Rangga
AU  - Tabu CR
AD  - Department of Pathology, Faculty of Veterinary Medicine, Gadjah Mada University, 
      Yogyakarta, Indonesia.
LA  - eng
PT  - Journal Article
DEP - 20150716
PL  - India
TA  - Vet World
JT  - Veterinary world
JID - 101504872
PMC - PMC4774680
OTO - NOTNLM
OT  - Indonesia
OT  - North Sumatera
OT  - open reading frame 7
OT  - porcine reproductive and respiratory syndrome virus
EDAT- 2016/04/06 06:00
MHDA- 2016/04/06 06:01
PMCR- 2015/07/01
CRDT- 2016/04/06 06:00
PHST- 2015/03/11 00:00 [received]
PHST- 2015/06/13 00:00 [revised]
PHST- 2015/06/22 00:00 [accepted]
PHST- 2016/04/06 06:00 [entrez]
PHST- 2016/04/06 06:00 [pubmed]
PHST- 2016/04/06 06:01 [medline]
PHST- 2015/07/01 00:00 [pmc-release]
AID - 10.14202/vetworld.2015.875-880 [doi]
PST - ppublish
SO  - Vet World. 2015 Jul;8(7):875-80. doi: 10.14202/vetworld.2015.875-880. Epub 2015 
      Jul 16.