PMID- 27047509
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20160406
LR  - 20200930
IS  - 1664-462X (Print)
IS  - 1664-462X (Electronic)
IS  - 1664-462X (Linking)
VI  - 7
DP  - 2016
TI  - Activation of Shikimate, Phenylpropanoid, Oxylipins, and Auxin Pathways in 
      Pectobacterium carotovorum Elicitors-Treated Moss.
PG  - 328
LID - 10.3389/fpls.2016.00328 [doi]
LID - 328
AB  - Plants have developed complex defense mechanisms to cope with microbial 
      pathogens. Pathogen-associated molecular patterns (PAMPs) and damage-associated 
      molecular patterns (DAMPs) are perceived by pattern recognition receptors (PRRs), 
      leading to the activation of defense. While substantial progress has been made in 
      understanding the activation of plant defense by PAMPs and DAMPs recognition in 
      tracheophytes, far less information exists on related processes in early 
      divergent plants like mosses. The aim of this study was to identify genes that 
      were induced in P. patens in response to elicitors of Pectobacterium carotovorum 
      subsp. carotovorum, using a cDNA suppression subtractive hybridization (SSH) 
      method. A total of 239 unigenes were identified, including genes involved in 
      defense responses related to the shikimate, phenylpropanoid, and oxylipin 
      pathways. The expression levels of selected genes related to these pathways were 
      analyzed using quantitative RT-PCR, confirming their rapid induction by P.c. 
      carotovorum derived elicitors. In addition, P. patens induced cell wall 
      reinforcement after elicitor treatment by incorporation of phenolic compounds, 
      callose deposition, and elevated expression of Dirigent-like encoding genes. 
      Small molecule defense markers and phytohormones such as cinnamic acid, 
      12-oxo-phytodienoic acid, and auxin levels all increased in elicitor-treated moss 
      tissues. In contrast, salicylic acid levels decreased while abscisic acid levels 
      remained unchanged. P. patens reporter lines harboring an auxin-inducible 
      promoter fused to beta-glucuronidase revealed GUS activity in protonemal and 
      gametophores tissues treated with elicitors of P.c. carotovorum, consistent with 
      a localized activation of auxin signaling. These results indicate that P. patens 
      activates the shikimate, phenylpropanoid, oxylipins, and auxin pathways upon 
      treatment with P.c. carotovorum derived elicitors.
FAU - Alvarez, Alfonso
AU  - Alvarez A
AD  - Departamento de Biologia Molecular, Instituto de Investigaciones Biologicas 
      Clemente EstableMontevideo, Uruguay; Laboratorio de Fisiologia Vegetal, Facultad 
      de Ciencias, Centro de Investigaciones Nucleares, Universidad de la 
      RepublicaMontevideo, Uruguay.
FAU - Montesano, Marcos
AU  - Montesano M
AD  - Laboratorio de Fisiologia Vegetal, Facultad de Ciencias, Centro de 
      Investigaciones Nucleares, Universidad de la Republica Montevideo, Uruguay.
FAU - Schmelz, Eric
AU  - Schmelz E
AD  - Section of Cell and Developmental Biology, University of California San Diego, La 
      Jolla, CA, USA.
FAU - Ponce de Leon, Ines
AU  - Ponce de Leon I
AD  - Departamento de Biologia Molecular, Instituto de Investigaciones Biologicas 
      Clemente Estable Montevideo, Uruguay.
LA  - eng
PT  - Journal Article
DEP - 20160322
PL  - Switzerland
TA  - Front Plant Sci
JT  - Frontiers in plant science
JID - 101568200
PMC - PMC4801897
OTO - NOTNLM
OT  - Pectobacterium carotovorum
OT  - Physcomitrella patens
OT  - auxin
OT  - cell wall
OT  - defense
OT  - phenylpropanoid
EDAT- 2016/04/06 06:00
MHDA- 2016/04/06 06:01
PMCR- 2016/01/01
CRDT- 2016/04/06 06:00
PHST- 2015/12/30 00:00 [received]
PHST- 2016/03/03 00:00 [accepted]
PHST- 2016/04/06 06:00 [entrez]
PHST- 2016/04/06 06:00 [pubmed]
PHST- 2016/04/06 06:01 [medline]
PHST- 2016/01/01 00:00 [pmc-release]
AID - 10.3389/fpls.2016.00328 [doi]
PST - epublish
SO  - Front Plant Sci. 2016 Mar 22;7:328. doi: 10.3389/fpls.2016.00328. eCollection 
      2016.