PMID- 27047962
OWN - NLM
STAT- MEDLINE
DCOM- 20161216
LR  - 20220409
IS  - 2314-6141 (Electronic)
IS  - 2314-6133 (Print)
VI  - 2016
DP  - 2016
TI  - Human Umbilical Cord Mesenchymal Stem Cells Therapy in Cyclophosphamide-Induced 
      Premature Ovarian Failure Rat Model.
PG  - 2517514
LID - 10.1155/2016/2517514 [doi]
LID - 2517514
AB  - Premature ovarian failure (POF) is one of the most common causes of infertility 
      in women. In our present study, we established cyclophosphamide- (CTX-) induced 
      POF rat model and elucidated its effect on ovarian function. We detected the 
      serum estrogen, follicle stimulating hormone, and anti-Mullerian hormone of mice 
      models by ELISA and evaluated their folliculogenesis by histopathology 
      examination. Our study revealed that CTX administration could severely disturb 
      hormone secretion and influence folliculogenesis in rat. This study also detected 
      ovarian cells apoptosis by deoxy-UTP-digoxigenin nick end labeling (TUNEL) and 
      demonstrated marked ovarian cells apoptosis in rat models following CTX 
      administration. In order to explore the potential of human umbilical cord 
      mesenchymal stem cells (UCMSCs) in POF treatment, the above indexes were used to 
      evaluate ovarian function. We found that human UCMSCs transplantation recovered 
      disturbed hormone secretion and folliculogenesis in POF rat, in addition to 
      reduced ovarian cell apoptosis. We also tracked transplanted UCMSCs in ovaries by 
      fluorescence in situ hybridization (FISH). The results manifested that the 
      transplanted human UCMSCs could reside in ovarian tissues and could survive for a 
      comparatively long time without obvious proliferation. Our present study provides 
      new insights into the great clinical potential of human UCMSCs in POF treatment.
FAU - Song, Dan
AU  - Song D
AD  - Center for Reproduction and Genetics, Suzhou Municipal Hospital, Nanjing Medical 
      University Affiliated Suzhou Hospital, Suzhou, Jiangsu 215002, China.
FAU - Zhong, Yun
AU  - Zhong Y
AD  - Center for Reproduction and Genetics, Suzhou Municipal Hospital, Nanjing Medical 
      University Affiliated Suzhou Hospital, Suzhou, Jiangsu 215002, China.
FAU - Qian, Chunfeng
AU  - Qian C
AD  - Center for Reproduction and Genetics, Suzhou Municipal Hospital, Nanjing Medical 
      University Affiliated Suzhou Hospital, Suzhou, Jiangsu 215002, China.
FAU - Zou, Qinyan
AU  - Zou Q
AD  - Center for Reproduction and Genetics, Suzhou Municipal Hospital, Nanjing Medical 
      University Affiliated Suzhou Hospital, Suzhou, Jiangsu 215002, China.
FAU - Ou, Jian
AU  - Ou J
AD  - Center for Reproduction and Genetics, Suzhou Municipal Hospital, Nanjing Medical 
      University Affiliated Suzhou Hospital, Suzhou, Jiangsu 215002, China.
FAU - Shi, Yichao
AU  - Shi Y
AD  - Center for Reproduction and Genetics, Suzhou Municipal Hospital, Nanjing Medical 
      University Affiliated Suzhou Hospital, Suzhou, Jiangsu 215002, China.
FAU - Gao, Liang
AU  - Gao L
AD  - Center for Reproduction and Genetics, Suzhou Municipal Hospital, Nanjing Medical 
      University Affiliated Suzhou Hospital, Suzhou, Jiangsu 215002, China.
FAU - Wang, Gaigai
AU  - Wang G
AD  - Center for Reproduction and Genetics, Suzhou Municipal Hospital, Nanjing Medical 
      University Affiliated Suzhou Hospital, Suzhou, Jiangsu 215002, China.
FAU - Liu, Zhenxing
AU  - Liu Z
AD  - Center for Reproduction and Genetics, Suzhou Municipal Hospital, Nanjing Medical 
      University Affiliated Suzhou Hospital, Suzhou, Jiangsu 215002, China.
FAU - Li, Haibo
AU  - Li H
AD  - Center for Reproduction and Genetics, Suzhou Municipal Hospital, Nanjing Medical 
      University Affiliated Suzhou Hospital, Suzhou, Jiangsu 215002, China.
FAU - Ding, Hailei
AU  - Ding H
AD  - Jiangsu Beike Biotechnology Co., Ltd., Jiangsu 215002, China.
FAU - Wu, Huihua
AU  - Wu H
AD  - Center for Reproduction and Genetics, Suzhou Municipal Hospital, Nanjing Medical 
      University Affiliated Suzhou Hospital, Suzhou, Jiangsu 215002, China.
FAU - Wang, Fuxin
AU  - Wang F
AD  - Center for Reproduction and Genetics, Suzhou Municipal Hospital, Nanjing Medical 
      University Affiliated Suzhou Hospital, Suzhou, Jiangsu 215002, China.
FAU - Wang, Jing
AU  - Wang J
AD  - Experimental Animal Center, Soochow University, Suzhou, Jiangsu 215006, China.
FAU - Li, Hong
AU  - Li H
AUID- ORCID: 0000-0002-6186-2152
AD  - Center for Reproduction and Genetics, Suzhou Municipal Hospital, Nanjing Medical 
      University Affiliated Suzhou Hospital, Suzhou, Jiangsu 215002, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20160307
PL  - United States
TA  - Biomed Res Int
JT  - BioMed research international
JID - 101600173
RN  - 8N3DW7272P (Cyclophosphamide)
SB  - IM
MH  - Animals
MH  - Apoptosis/drug effects
MH  - Cyclophosphamide/toxicity
MH  - Disease Models, Animal
MH  - Female
MH  - Humans
MH  - *Mesenchymal Stem Cell Transplantation
MH  - Mice
MH  - Ovary/pathology
MH  - Primary Ovarian Insufficiency/chemically induced/*surgery
MH  - Rats
MH  - Rats, Wistar
MH  - Umbilical Cord/*cytology
PMC - PMC4800076
EDAT- 2016/04/06 06:00
MHDA- 2016/12/17 06:00
PMCR- 2016/03/07
CRDT- 2016/04/06 06:00
PHST- 2015/12/17 00:00 [received]
PHST- 2016/01/27 00:00 [revised]
PHST- 2016/02/02 00:00 [accepted]
PHST- 2016/04/06 06:00 [entrez]
PHST- 2016/04/06 06:00 [pubmed]
PHST- 2016/12/17 06:00 [medline]
PHST- 2016/03/07 00:00 [pmc-release]
AID - 10.1155/2016/2517514 [doi]
PST - ppublish
SO  - Biomed Res Int. 2016;2016:2517514. doi: 10.1155/2016/2517514. Epub 2016 Mar 7.