PMID- 27050419
OWN - NLM
STAT- MEDLINE
DCOM- 20170522
LR  - 20171106
IS  - 1872-8359 (Electronic)
IS  - 0167-7012 (Linking)
VI  - 125
DP  - 2016 Jun
TI  - A quick colorimetric method for total lipid quantification in microalgae.
PG  - 28-32
LID - S0167-7012(16)30054-9 [pii]
LID - 10.1016/j.mimet.2016.04.002 [doi]
AB  - Discovering microalgae with high lipid productivity are among the key milestones 
      for achieving sustainable biodiesel production. Current methods of lipid 
      quantification are time intensive and costly. A rapid colorimetric method based 
      on sulfo-phospho-vanillin (SPV) reaction was developed for the quantification of 
      microbial lipids to facilitate screening for lipid producing microalgae. This 
      method was successfully tested on marine thraustochytrid strains and vegetable 
      oils. The colorimetric method results correlated well with gravimetric method 
      estimates. The new method was less time consuming than gravimetric analysis and 
      is quantitative for lipid determination, even in the presence of carbohydrates, 
      proteins and glycerol.
CI  - Copyright (c) 2016 Elsevier B.V. All rights reserved.
FAU - Byreddy, Avinesh R
AU  - Byreddy AR
AD  - Centre for Chemistry and Biotechnology, School of Life and Environmental 
      Sciences, Waurn Ponds, Geelong, Deakin University, Victoria 3217, Australia.
FAU - Gupta, Adarsha
AU  - Gupta A
AD  - Centre for Chemistry and Biotechnology, School of Life and Environmental 
      Sciences, Waurn Ponds, Geelong, Deakin University, Victoria 3217, Australia.
FAU - Barrow, Colin J
AU  - Barrow CJ
AD  - Centre for Chemistry and Biotechnology, School of Life and Environmental 
      Sciences, Waurn Ponds, Geelong, Deakin University, Victoria 3217, Australia.
FAU - Puri, Munish
AU  - Puri M
AD  - Centre for Chemistry and Biotechnology, School of Life and Environmental 
      Sciences, Waurn Ponds, Geelong, Deakin University, Victoria 3217, Australia. 
      Electronic address: munish.puri@deakin.edu.au.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20160402
PL  - Netherlands
TA  - J Microbiol Methods
JT  - Journal of microbiological methods
JID - 8306883
RN  - 0 (Benzaldehydes)
RN  - 0 (Biofuels)
RN  - 0 (Lipids)
RN  - 0 (Plant Oils)
RN  - CHI530446X (vanillin)
SB  - IM
MH  - Benzaldehydes
MH  - Biofuels
MH  - Colorimetry/*methods
MH  - Data Accuracy
MH  - Esterification
MH  - Industrial Microbiology
MH  - Lipids/*analysis
MH  - Microalgae/*chemistry
MH  - Plant Oils/chemistry
OTO - NOTNLM
OT  - Algae
OT  - Biodiesel
OT  - Esterification
OT  - Industrial biotechnology
OT  - Vanillin
EDAT- 2016/04/07 06:00
MHDA- 2017/05/23 06:00
CRDT- 2016/04/07 06:00
PHST- 2016/03/02 00:00 [received]
PHST- 2016/04/01 00:00 [revised]
PHST- 2016/04/01 00:00 [accepted]
PHST- 2016/04/07 06:00 [entrez]
PHST- 2016/04/07 06:00 [pubmed]
PHST- 2017/05/23 06:00 [medline]
AID - S0167-7012(16)30054-9 [pii]
AID - 10.1016/j.mimet.2016.04.002 [doi]
PST - ppublish
SO  - J Microbiol Methods. 2016 Jun;125:28-32. doi: 10.1016/j.mimet.2016.04.002. Epub 
      2016 Apr 2.