PMID- 27054573
OWN - NLM
STAT- MEDLINE
DCOM- 20160825
LR  - 20190219
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 11
IP  - 4
DP  - 2016
TI  - Stability of the Encoding Plasmids and Surface Expression of CS6 Differs in 
      Enterotoxigenic Escherichia coli (ETEC) Encoding Different Heat-Stable (ST) 
      Enterotoxins (STh and STp).
PG  - e0152899
LID - 10.1371/journal.pone.0152899 [doi]
LID - e0152899
AB  - Enterotoxigenic Escherichia coli (ETEC), one of the most common reasons of 
      diarrhea among infants and children in developing countries, causes disease by 
      expression of either or both of the enterotoxins heat-labile (LT) and heat-stable 
      (ST; divided into human-type [STh] and porcine-type [STp] variants), and 
      colonization factors (CFs) among which CS6 is one of the most prevalent ETEC CFs. 
      In this study we show that ETEC isolates expressing CS6+STh have higher copy 
      numbers of the cssABCD operon encoding CS6 than those expressing CS6+STp. Long 
      term cultivation of up to ten over-night passages of ETEC isolates harboring 
      CS6+STh (n = 10) or CS6+STp (n = 15) showed instability of phenotypic expression 
      of CS6 in a majority of the CS6+STp isolates, whereas most of the CS6+STh 
      isolates retained CS6 expression. The observed instability was a correlated with 
      loss of genes cssA and cssD as examined by PCR. Mobilization of the CS6 plasmid 
      from an unstable CS6+STp isolate into a laboratory E. coli strain resulted in 
      loss of the plasmid after a single over-night passage whereas the plasmid from an 
      CS6+STh strain was retained in the laboratory strain during 10 passages. A 
      sequence comparison between the CS6 plasmids from a stable and an unstable ETEC 
      isolate revealed that genes necessary for plasmid stabilization, for example 
      pemI, pemK, stbA, stbB and parM, were not present in the unstable ETEC isolate. 
      Our results indicate that stable retention of CS6 may in part be affected by the 
      stability of the plasmid on which both CS6 and STp or STh are located.
FAU - Tobias, Joshua
AU  - Tobias J
AD  - Department of Microbiology and Immunology, Institute of Biomedicine, University 
      of Gothenburg, S-40530, Goteborg, Sweden.
FAU - Von Mentzer, Astrid
AU  - Von Mentzer A
AD  - Department of Microbiology and Immunology, Institute of Biomedicine, University 
      of Gothenburg, S-40530, Goteborg, Sweden.
FAU - Loayza Frykberg, Patricia
AU  - Loayza Frykberg P
AD  - Department of Microbiology and Immunology, Institute of Biomedicine, University 
      of Gothenburg, S-40530, Goteborg, Sweden.
FAU - Aslett, Martin
AU  - Aslett M
AD  - Pathogen Genomics, Wellcome Trust Sanger Institute, Hinxton, Cambridge, CB10 1SA, 
      United Kingdom.
FAU - Page, Andrew J
AU  - Page AJ
AD  - Pathogen Genomics, Wellcome Trust Sanger Institute, Hinxton, Cambridge, CB10 1SA, 
      United Kingdom.
FAU - Sjoling, Asa
AU  - Sjoling A
AD  - Department of Microbiology and Immunology, Institute of Biomedicine, University 
      of Gothenburg, S-40530, Goteborg, Sweden.
AD  - Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet, S-171 
      77, Stockholm, Sweden.
FAU - Svennerholm, Ann-Mari
AU  - Svennerholm AM
AD  - Department of Microbiology and Immunology, Institute of Biomedicine, University 
      of Gothenburg, S-40530, Goteborg, Sweden.
LA  - eng
GR  - Wellcome Trust/United Kingdom
GR  - WT 098051/Wellcome Trust/United Kingdom
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20160407
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (Enterotoxins)
RN  - 0 (Escherichia coli Proteins)
SB  - IM
MH  - Enterotoxigenic Escherichia coli/genetics/isolation & 
      purification/*metabolism/pathogenicity
MH  - Enterotoxins/chemistry/*metabolism
MH  - Escherichia coli Infections/genetics
MH  - Escherichia coli Proteins/genetics/*metabolism
MH  - Hot Temperature
MH  - Humans
MH  - Mutation/genetics
MH  - Operon
MH  - Plasmids/*chemistry/genetics
MH  - Real-Time Polymerase Chain Reaction
PMC - PMC4824445
COIS- Competing Interests: The authors have declared that no competing interests exist.
EDAT- 2016/04/08 06:00
MHDA- 2016/08/26 06:00
PMCR- 2016/04/07
CRDT- 2016/04/08 06:00
PHST- 2015/12/23 00:00 [received]
PHST- 2016/03/21 00:00 [accepted]
PHST- 2016/04/08 06:00 [entrez]
PHST- 2016/04/08 06:00 [pubmed]
PHST- 2016/08/26 06:00 [medline]
PHST- 2016/04/07 00:00 [pmc-release]
AID - PONE-D-15-55751 [pii]
AID - 10.1371/journal.pone.0152899 [doi]
PST - epublish
SO  - PLoS One. 2016 Apr 7;11(4):e0152899. doi: 10.1371/journal.pone.0152899. 
      eCollection 2016.