PMID- 27055186
OWN - NLM
STAT- MEDLINE
DCOM- 20170522
LR  - 20181202
IS  - 1530-0447 (Electronic)
IS  - 0031-3998 (Linking)
VI  - 80
IP  - 2
DP  - 2016 Aug
TI  - Cysteinyl-glycine reduces mucosal proinflammatory cytokine response to fMLP in a 
      parenterally-fed piglet model.
PG  - 293-8
LID - 10.1038/pr.2016.69 [doi]
AB  - BACKGROUND: PepT1 transports dietary and bacterial peptides in the gut. We 
      hypothesized that cysteinyl-glycine would ameliorate the inflammatory effect of a 
      bacterial peptide, formyl-methionyl-leucyl-phenylalanine (fMLP), in both sow-fed 
      and parenterally-fed piglets. METHODS: An intestinal perfusion experiment was 
      performed in piglets (N = 12) that were sow-reared or provided with parenteral 
      nutrition (PN) for 4 d. In each piglet, five segments of isolated intestine were 
      perfused with five treatments including cysteine and glycine, cysteinyl-glycine, 
      fMLP, free cysteine and glycine with fMLP, or cysteinyl-glycine with fMLP. 
      Mucosal cytokine responses and intestinal morphology was assessed in each gut 
      segment. RESULTS: PN piglets had lower mucosal IL-10 by approximately 20% (P < 
      0.01). Cysteinyl-glycine lowered TNF-alpha response to fMLP in PN-fed animals and 
      IFN-gamma response to fMLP in both groups (P < 0.05). The free cysteine and glycine 
      treatment reduced TNF-alpha in sow-fed animals (P < 0.05). fMLP affected villus 
      height in parenterally (P < 0.05), but not sow-fed animals. CONCLUSION: 
      Parenteral feeding conferred a susceptibility to mucosal damage by fMLP. The 
      dipeptide was more effective at attenuating the inflammatory response to a 
      bacterial peptide than free amino acids. This may be due to competitive 
      inhibition of fMLP transport or a greater efficiency of transport of dipeptides.
FAU - Nosworthy, Matthew G
AU  - Nosworthy MG
AD  - Department of Biochemistry, Memorial University of Newfoundland, St. John's, 
      Newfoundland, Canada.
FAU - Brunton, Janet A
AU  - Brunton JA
AD  - Department of Biochemistry, Memorial University of Newfoundland, St. John's, 
      Newfoundland, Canada.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20160407
PL  - United States
TA  - Pediatr Res
JT  - Pediatric research
JID - 0100714
RN  - 0 (Cytokines)
RN  - 0 (Dipeptides)
RN  - 130068-27-8 (Interleukin-10)
RN  - 384644SZ9T (cysteinylglycine)
RN  - 3OWL53L36A (Mannitol)
RN  - 59880-97-6 (N-Formylmethionine Leucyl-Phenylalanine)
RN  - EC 1.11.1.7 (Peroxidase)
RN  - K848JZ4886 (Cysteine)
RN  - TE7660XO1C (Glycine)
SB  - IM
MH  - Animals
MH  - Cysteine/chemistry
MH  - Cytokines/*metabolism
MH  - Dipeptides/*chemistry
MH  - Disease Models, Animal
MH  - Genetic Predisposition to Disease
MH  - Glycine/chemistry
MH  - Inflammation/*metabolism
MH  - Interleukin-10/metabolism
MH  - Intestinal Mucosa/metabolism
MH  - Mannitol/chemistry
MH  - Mucous Membrane/*metabolism
MH  - N-Formylmethionine Leucyl-Phenylalanine/*chemistry
MH  - Parenteral Nutrition
MH  - Perfusion
MH  - Peroxidase/metabolism
MH  - Random Allocation
MH  - Swine
MH  - Time Factors
EDAT- 2016/04/08 06:00
MHDA- 2017/05/23 06:00
CRDT- 2016/04/08 06:00
PHST- 2015/09/23 00:00 [received]
PHST- 2016/02/02 00:00 [accepted]
PHST- 2016/04/08 06:00 [entrez]
PHST- 2016/04/08 06:00 [pubmed]
PHST- 2017/05/23 06:00 [medline]
AID - pr201669 [pii]
AID - 10.1038/pr.2016.69 [doi]
PST - ppublish
SO  - Pediatr Res. 2016 Aug;80(2):293-8. doi: 10.1038/pr.2016.69. Epub 2016 Apr 7.