PMID- 27055862
OWN - NLM
STAT- MEDLINE
DCOM- 20180104
LR  - 20180131
IS  - 1464-5157 (Electronic)
IS  - 0265-6736 (Linking)
VI  - 32
IP  - 5
DP  - 2016 Aug
TI  - Punicalagin induces Nrf2 translocation and HO-1 expression via PI3K/Akt, 
      protecting rat intestinal epithelial cells from oxidative stress.
PG  - 465-73
LID - 10.3109/02656736.2016.1155762 [doi]
AB  - PURPOSE: Oxidative stress plays a central role in heat stress-induced 
      gastrointestinal injury. Punicalagin (PUN), a major polyphenol abundant in 
      pomegranate fruit, husk and juice, exhibits antioxidative effects. In this study 
      we used a heat stress model to investigate the intestinal protection effect of 
      PUN and the underlying mechanisms. MATERIALS AND METHODS: IEC-6 cells were 
      pretreated with PUN for 6 h and exposed to 42  degrees C for 6 h. Intracellular reactive 
      oxygen species levels, malondialdehyde, nitrogen oxide, and superoxide dismutase 
      activity were measured. IEC-6 cells were treated with PUN at different times and 
      doses, the protein levels of haeme oxygenase-1 (HO-1) were evaluated. The nuclear 
      translocation of the transcription factor NF-erythroid 2-related factor (Nrf2) 
      and the phosphorylation level of PI3K/Akt were also investigated. RESULTS: PUN 
      significantly decreased the heat stress-induced cell death and apoptosis. Heat 
      stress increased reactive oxygen species, malondialdehyde and nitrogen oxide 
      production, while it decreased superoxide dismutase activity. These effects were 
      markedly inversed when the cells were pretreated with PUN. Furthermore, PUN 
      treatment induced the expression of HO-1 and increased Nrf2 nuclear translocation 
      in a time- and dose-dependent manner. The Nrf2-related cytoprotective effects of 
      PUN were via the PI3K/Akt signalling pathway, as LY294002, a specific PI3K/Akt 
      inhibitor, suppressed the PUN-induced nuclear translocation of Nrf2, the HO-1 
      up-regulation and the protective effect of PUN against oxidative stress. 
      CONCLUSIONS: PUN protects IEC-6 cells against oxidative stress by up-regulating 
      the expression of HO-1 via a mechanism that involves PI3K/Akt activation and Nrf2 
      translocation.
FAU - Xu, Lei
AU  - Xu L
AD  - a Beijing Key Laboratory for Dairy Cow Nutrition , College of Animal Science and 
      Technology, Beijing University of Agriculture , Beijing ;
FAU - He, ShaSha
AU  - He S
AD  - b College of Veterinary Medicine , China Agricultural University (CAU) , Beijing 
      , China.
FAU - Yin, Peng
AU  - Yin P
AD  - b College of Veterinary Medicine , China Agricultural University (CAU) , Beijing 
      , China.
FAU - Li, DeYin
AU  - Li D
AD  - a Beijing Key Laboratory for Dairy Cow Nutrition , College of Animal Science and 
      Technology, Beijing University of Agriculture , Beijing ;
FAU - Mei, Chen
AU  - Mei C
AD  - a Beijing Key Laboratory for Dairy Cow Nutrition , College of Animal Science and 
      Technology, Beijing University of Agriculture , Beijing ;
FAU - Yu, XiaoHong
AU  - Yu X
AD  - a Beijing Key Laboratory for Dairy Cow Nutrition , College of Animal Science and 
      Technology, Beijing University of Agriculture , Beijing ;
FAU - Shi, YaRan
AU  - Shi Y
AD  - a Beijing Key Laboratory for Dairy Cow Nutrition , College of Animal Science and 
      Technology, Beijing University of Agriculture , Beijing ;
FAU - Jiang, LinShu
AU  - Jiang L
AD  - a Beijing Key Laboratory for Dairy Cow Nutrition , College of Animal Science and 
      Technology, Beijing University of Agriculture , Beijing ;
FAU - Liu, FengHua
AU  - Liu F
AD  - a Beijing Key Laboratory for Dairy Cow Nutrition , College of Animal Science and 
      Technology, Beijing University of Agriculture , Beijing ;
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20160407
PL  - England
TA  - Int J Hyperthermia
JT  - International journal of hyperthermia : the official journal of European Society 
      for Hyperthermic Oncology, North American Hyperthermia Group
JID - 8508395
RN  - 0 (Antioxidants)
RN  - 0 (Hydrolyzable Tannins)
RN  - 0 (NF-E2-Related Factor 2)
RN  - 0 (Nfe2l2 protein, rat)
RN  - 0 (Reactive Oxygen Species)
RN  - 31C4KY9ESH (Nitric Oxide)
RN  - 65995-63-3 (punicalagin)
RN  - EC 1.14.14.18 (Heme Oxygenase (Decyclizing))
RN  - EC 1.14.14.18 (Hmox1 protein, rat)
RN  - EC 1.15.1.1 (Superoxide Dismutase)
RN  - EC 2.7.1.- (Phosphatidylinositol 3-Kinases)
RN  - EC 2.7.11.1 (Proto-Oncogene Proteins c-akt)
SB  - IM
MH  - Animals
MH  - Antioxidants/*pharmacology
MH  - Cell Line
MH  - Cell Survival
MH  - Epithelial Cells/*drug effects/metabolism
MH  - Heme Oxygenase (Decyclizing)/metabolism
MH  - Hot Temperature/*adverse effects
MH  - Hydrolyzable Tannins/*pharmacology
MH  - Intestines/*cytology
MH  - NF-E2-Related Factor 2/metabolism
MH  - Nitric Oxide/metabolism
MH  - Oxidative Stress/drug effects
MH  - Phosphatidylinositol 3-Kinases/metabolism
MH  - Proto-Oncogene Proteins c-akt/metabolism
MH  - Rats
MH  - Reactive Oxygen Species/metabolism
MH  - Superoxide Dismutase/metabolism
OTO - NOTNLM
OT  - Cytoprotection
OT  - Nrf2
OT  - oxidative stress
OT  - punicalagin
EDAT- 2016/04/09 06:00
MHDA- 2018/01/05 06:00
CRDT- 2016/04/09 06:00
PHST- 2016/04/09 06:00 [entrez]
PHST- 2016/04/09 06:00 [pubmed]
PHST- 2018/01/05 06:00 [medline]
AID - 10.3109/02656736.2016.1155762 [doi]
PST - ppublish
SO  - Int J Hyperthermia. 2016 Aug;32(5):465-73. doi: 10.3109/02656736.2016.1155762. 
      Epub 2016 Apr 7.