PMID- 27058019
OWN - NLM
STAT- MEDLINE
DCOM- 20170414
LR  - 20220330
IS  - 1557-8550 (Electronic)
IS  - 1549-5418 (Linking)
VI  - 34
IP  - 11
DP  - 2016 Nov
TI  - Effects of Photobiomodulation and Mesenchymal Stem Cells on Articular Cartilage 
      Defects in a Rabbit Model.
PG  - 543-549
AB  - OBJECTIVE: The aim of this study was to evaluate the effectiveness of the 
      application of cultured autologous bone marrow mesenchymal stem cells (BMSCs) 
      with scaffold and low-level laser therapy (LLLT) on the repair of articular 
      cartilage defects in rabbits. BACKGROUND DATA: For healing of the articular 
      cartilage defects, although positive effects of BMSCs and LLLT have been 
      demonstrated, their combination effect is still unknown; therefore, we 
      investigated combining these two techniques has a synergistic effect. MATERIALS 
      AND METHODS: After bone marrow aspiration from 10 rabbits, BMSCs were isolated, 
      cultured in monolayer, suspended on a type I collagen scaffold and then implanted 
      onto a full-thickness osteochondral defect (4 mm in diameter), artificially made 
      on the patellar groove of both knees in the same rabbits. Then a knee was 
      selected randomly in each rabbit as the experimental group, and subjected to 
      Ga-Al-As (810 nm) laser irradiation with energy density of 4 J/cm(2) every other 
      day for 3 weeks. As the control group, the other knee did not receive LLLT. After 
      this period, animals were euthanized and osteochondral defects were evaluated by 
      histomorphometric methods. RESULTS: No significant difference in new cartilage 
      formation and inflammation was found between the groups (p > 0.05). However, 
      there was significantly more new bone formation in the experimental group (p < 
      0.05). CONCLUSIONS: In terms of our research, although better healing in 
      osteochondral defects was seen when combining BMSCs and LLLT compared with the 
      use of BMSCs alone, this improvement was predominantly caused by new bone 
      formation rather than new cartilage formation.
FAU - Fekrazad, Reza
AU  - Fekrazad R
AD  - 1 Department of Periodontology, Dental Faculty, AJA University of Medical 
      Sciences , Tehran, Iran .
FAU - Eslaminejad, Mohamadreza Baghaban
AU  - Eslaminejad MB
AD  - 2 Department of Stem Cells and Developmental Biology, Cell Science Research 
      Center, Royan Institute for Stem Cell Biology and Technology , ACECR, Tehran, 
      Iran .
FAU - Shayan, Arman M
AU  - Shayan AM
AD  - 3 Department of Orthodontics, Faculty of Dentistry, Tabriz University of Medical 
      Sciences , Tabriz, Iran .
FAU - Kalhori, Katayoun A M
AU  - Kalhori KA
AD  - 4 Oral & Maxillofacial Pathologist, Iranian Medical Laser Association, Tehran, 
      Iran .
FAU - Abbas, Fatemeh Mashhadi
AU  - Abbas FM
AD  - 5 Department of Oral & Maxillofacial Pathology, Shahid Beheshti University of 
      Medical Sciences , Tehran, Iran .
FAU - Taghiyar, Leila
AU  - Taghiyar L
AD  - 6 Department of Stem Cells and Developmental Biology, Cell Sciences Research 
      Center, Royan Institute for Stem Cell Biology and Technology , ACECR, Tehran, 
      Iran .
FAU - Sepehr Pedram, Mir
AU  - Sepehr Pedram M
AD  - 7 Department of Surgery & Radiology, Faculty of Veterinary Medicine, University 
      of Tehran , Tehran, Iran .
FAU - Ghuchani, Mostafa Sadeghi
AU  - Ghuchani MS
AD  - 8 Department of Orthodontics, Gorgan Faculty of Dentistry, Golestan University of 
      Medical Sciences , Golestan, Iran .
LA  - eng
PT  - Journal Article
DEP - 20160408
PL  - United States
TA  - Photomed Laser Surg
JT  - Photomedicine and laser surgery
JID - 101222340
SB  - IM
MH  - Analysis of Variance
MH  - Animals
MH  - Biopsy, Needle
MH  - Bone Marrow Cells/radiation effects
MH  - Bone Marrow Transplantation/*methods
MH  - Cartilage, Articular/injuries/pathology/*radiation effects
MH  - Cells, Cultured
MH  - Disease Models, Animal
MH  - Immunohistochemistry
MH  - Knee Joint/pathology/radiation effects
MH  - Low-Level Light Therapy/*methods
MH  - Male
MH  - Mesenchymal Stem Cells/*radiation effects
MH  - Rabbits
MH  - Random Allocation
MH  - Regeneration/physiology/radiation effects
MH  - Treatment Outcome
OTO - NOTNLM
OT  - articular cartilage repair
OT  - bone marrow mesenchymal stem cells
OT  - low-level laser therapy
OT  - osteochondral defect
OT  - photobiomodulation
OT  - rabbit model
EDAT- 2016/04/09 06:00
MHDA- 2017/04/15 06:00
CRDT- 2016/04/09 06:00
PHST- 2016/04/09 06:00 [pubmed]
PHST- 2017/04/15 06:00 [medline]
PHST- 2016/04/09 06:00 [entrez]
AID - 10.1089/pho.2015.4028 [doi]
PST - ppublish
SO  - Photomed Laser Surg. 2016 Nov;34(11):543-549. doi: 10.1089/pho.2015.4028. Epub 
      2016 Apr 8.