PMID- 27060321
OWN - NLM
STAT- MEDLINE
DCOM- 20160630
LR  - 20160410
IS  - 1003-9406 (Print)
IS  - 1003-9406 (Linking)
VI  - 33
IP  - 2
DP  - 2016 Apr
TI  - [Karyotype analysis and fluorescence in situ hybridization detection of 122 
      patients with myelodysplastic syndrome].
PG  - 221-6
LID - 10.3760/cma.j.issn.1003-9406.2016.02.021 [doi]
AB  - OBJECTIVE: To assess the value of karyotype analysis and fluorescence in situ 
      hybridization(FISH) assay for the diagnosis of myelodysplastic syndrome (MDS). 
      METHODS: The karyotypes of 122 initially treated MDS patients were analyzed with 
      conventional R-banding and FISH using probes including GLP CSF1R/D5S23, D5S721, 
      GLP EGR1/D5S23, D5S721, GLP D7S486/CSP7, GLP D7S522/CSP7, GLP D20S108, CSP8 and 
      CSP X/Y. RESULTS: The detection rate of chromosomal abnormalities was 54.9% for 
      the 122 patients. Among these, those involving 3 or more chromosomes are most 
      common (16.4%), followed by +8(14.8%), -7/7q-(7.4%), -5/5q-(5.7%), 20q-(2.5%), 
      and -Y in male patients (5.0%). Two MDS-RAEB II patients detected with t(8;21) 
      should be diagnosed with acute myelocytic leukemia. FISH analysis showed that 54 
      patients were positive (44.3%). Among these, 30.3% had CSP8 amplification, 
      followed by GLP D7S486/CSP7 and GLP D7S522/CSP7 deletion (12.3%), GLP 
      CSF1R/D5S23, D5S721 and GLP EGR1/D5S23, D5S721 deletion (9.8%), GLP D20S108 
      deletion (7.4%), and CSPX/Y deletion (5%). CONCLUSION: With a detection rate of 
      54.9%, R-banding still constitutes the basic examination for MDS. As detection of 
      interstitial chromosomal abnormalities in MDS can be greatly enhanced by FISH, 
      combined karyotype analysis and FISH can improve the diagnosis of MDS and 
      facilitate assessment of its prognosis.
FAU - Jing, Yuan
AU  - Jing Y
AD  - Department of Hematology, the First Affiliated Hospital, Dalian Medical 
      University, Dalian, Liaoning 116011, China. jingyuanan@aliyun.com.
FAU - Lin, Shuang
AU  - Lin S
FAU - Jiang, Feng
AU  - Jiang F
FAU - Wang, Fangting
AU  - Wang F
FAU - Fang, Meiyun
AU  - Fang M
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Zhonghua Yi Xue Yi Chuan Xue Za Zhi
JT  - Zhonghua yi xue yi chuan xue za zhi = Zhonghua yixue yichuanxue zazhi = Chinese 
      journal of medical genetics
JID - 9425197
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Child
MH  - Chromosome Aberrations
MH  - Chromosome Banding
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Karyotyping
MH  - Male
MH  - Middle Aged
MH  - Myelodysplastic Syndromes/*genetics
MH  - Sequence Deletion
MH  - Young Adult
EDAT- 2016/04/10 06:00
MHDA- 2016/07/01 06:00
CRDT- 2016/04/10 06:00
PHST- 2016/04/10 06:00 [entrez]
PHST- 2016/04/10 06:00 [pubmed]
PHST- 2016/07/01 06:00 [medline]
AID - 940632047 [pii]
AID - 10.3760/cma.j.issn.1003-9406.2016.02.021 [doi]
PST - ppublish
SO  - Zhonghua Yi Xue Yi Chuan Xue Za Zhi. 2016 Apr;33(2):221-6. doi: 
      10.3760/cma.j.issn.1003-9406.2016.02.021.