PMID- 27061405
OWN - NLM
STAT- MEDLINE
DCOM- 20170104
LR  - 20200422
IS  - 1096-9071 (Electronic)
IS  - 0146-6615 (Print)
IS  - 0146-6615 (Linking)
VI  - 88
IP  - 7
DP  - 2016 Jul
TI  - Comparison of Luminex xTAG(R) RVP fast assay and real time RT-PCR for the detection 
      of respiratory viruses in adults with community-acquired pneumonia.
PG  - 1173-9
LID - 10.1002/jmv.24463 [doi]
AB  - Community-acquired pneumonia (CAP) is the third cause of death worldwide. Viruses 
      are frequently detected in adult CAP. Highly sensitive diagnostic techniques 
      should be used due to poor viral shedding. Different sampling methods can affect 
      viral detection, being necessary to establish the optimal type of sample for 
      identifying respiratory viruses in adults. The detection rates of respiratory 
      viruses by Luminex xTAG(R) RVP fast assay, real time RT-PCR (rtRT-PCR) (Sacace(R)), 
      and immunofluorescence assay (IFA) in adult CAP were performed in nasopharyngeal 
      swabs (NPS) and aspirates (NPA) from 179 hospitalized adults. Positivity was 
      47.5% for Luminex(R), 42.5% for rtRT-PCR (P = 0.3), and 2.7% for IFA (2.7%) 
      (P < 0.0). The sensitivity, specificity, and kappa coefficient of xTAG(R) RVP 
      compared with rtRT-PCR were 84.2%, 79.6%, and 0.62%, respectively. Luminex(R) and 
      rtRT-PCR detected 65 (58.0%) and 57 (50.9%) viruses in 112 NPA and 35 (34.3%) and 
      31 (30.4%) in 102 NPS, respectively (P < 0.01). xTAG(R) RVP is appropriate for 
      detecting respiratory viruses in CAP adults. Both molecular techniques yielded 
      better results with nasopharyngeal aspirate than swabs.
CI  - (c) 2016 Wiley Periodicals, Inc.
FAU - Luchsinger, Vivian
AU  - Luchsinger V
AD  - Program of Virology, Faculty of Medicine, University of Chile, Santiago, Chile.
FAU - Prades, Yara
AU  - Prades Y
AD  - Program of Virology, Faculty of Medicine, University of Chile, Santiago, Chile.
FAU - Ruiz, Mauricio
AU  - Ruiz M
AD  - Clinical Hospital of the University of Chile, Santiago, Chile.
FAU - Pizarro, Rolando
AU  - Pizarro R
AD  - Dr. Lucio Cordova Hospital of Infectious Diseases, Santiago, Chile.
FAU - Rossi, Patricio
AU  - Rossi P
AD  - San Jose Hospital Complex, Santiago, Chile.
FAU - Lizama, Luis
AU  - Lizama L
AD  - Program of Virology, Faculty of Medicine, University of Chile, Santiago, Chile.
FAU - Garmendia, Maria Luisa
AU  - Garmendia ML
AD  - Institute of Nutrition and Food Technology, University of Chile, Santiago, Chile.
FAU - Meza, Angela
AU  - Meza A
AD  - San Jose Hospital Complex, Santiago, Chile.
FAU - Larranaga, Carmen
AU  - Larranaga C
AD  - Program of Virology, Faculty of Medicine, University of Chile, Santiago, Chile.
FAU - Avendano, Luis F
AU  - Avendano LF
AD  - Program of Virology, Faculty of Medicine, University of Chile, Santiago, Chile.
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20160202
PL  - United States
TA  - J Med Virol
JT  - Journal of medical virology
JID - 7705876
RN  - 0 (Reagent Kits, Diagnostic)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Community-Acquired Infections/*diagnosis/*virology
MH  - Female
MH  - Fluorescent Antibody Technique, Indirect
MH  - Hospitalization
MH  - Humans
MH  - Male
MH  - Middle Aged
MH  - Molecular Diagnostic Techniques/methods
MH  - Nasopharynx/virology
MH  - Pneumonia, Viral/*diagnosis/*virology
MH  - Reagent Kits, Diagnostic
MH  - Real-Time Polymerase Chain Reaction/*methods
MH  - Sensitivity and Specificity
MH  - Viruses/*isolation & purification
MH  - Young Adult
PMC - PMC7166572
OTO - NOTNLM
OT  - Luminex xTAG RVP
OT  - community-acquired pneumonia
OT  - diagnosis
OT  - polymerase chain reaction
OT  - respiratory viruses
EDAT- 2016/04/12 06:00
MHDA- 2017/01/05 06:00
PMCR- 2020/04/19
CRDT- 2016/04/11 06:00
PHST- 2015/12/26 00:00 [accepted]
PHST- 2016/04/11 06:00 [entrez]
PHST- 2016/04/12 06:00 [pubmed]
PHST- 2017/01/05 06:00 [medline]
PHST- 2020/04/19 00:00 [pmc-release]
AID - JMV24463 [pii]
AID - 10.1002/jmv.24463 [doi]
PST - ppublish
SO  - J Med Virol. 2016 Jul;88(7):1173-9. doi: 10.1002/jmv.24463. Epub 2016 Feb 2.