PMID- 27063491
OWN - NLM
STAT- MEDLINE
DCOM- 20171020
LR  - 20171117
IS  - 1878-7568 (Electronic)
IS  - 1742-7061 (Linking)
VI  - 41
DP  - 2016 Sep 1
TI  - Liposome-based intranasal delivery of lipopeptide vaccine candidates against 
      group A streptococcus.
PG  - 161-8
LID - S1742-7061(16)30165-9 [pii]
LID - 10.1016/j.actbio.2016.04.012 [doi]
AB  - Group A streptococcus (GAS), an exclusively human pathogen, causes a wide range 
      of diseases ranging from trivial to life threatening. Treatment of infection is 
      often ineffective following entry of bacteria into the bloodstream. To date, 
      there is no vaccine available against GAS. In this study, cationic liposomes 
      encapsulating lipopeptide-based vaccine candidates against GAS have been employed 
      for intranasal vaccine delivery. Cationic liposomes were prepared with 
      dimethyldioctadecylammonium bromide (DDAB) using the film hydration method. 
      Female Swiss mice were immunized intranasally with the liposomes. In contrast to 
      unmodified peptides, lipopeptides entrapped by liposomes induced both mucosal and 
      systemic immunity, IgA and IgG (IgG1 and IgG2a) production in mice, respectively. 
      High levels of antibody (IgA and IgG) titres were detected even five months post 
      immunization. Thus, the combination of lipopeptides and liposomes generates a 
      very promising delivery system for intranasal vaccines. STATEMENT OF 
      SIGNIFICANCE: Group A streptococcus, causing rheumatic heart diseases, kills 
      approximately half a million people annually. There is no vaccine available 
      against the infection. Mucosal immunity is vital in ensuring an individual is 
      protected as this gram positive bacteria initially colonizes at the throat. 
      Herein, we demonstrated that lipopeptides entrapped by liposomes induced both 
      mucosal and systemic immunity. High levels of antibody (IgA and IgG) titres were 
      detected even five months post immunization and lead vaccine candidate was able 
      to induce humoral immune responses even after single immunization. Thus, the 
      combination of lipopeptides and liposomes generates a very promising delivery 
      system for intranasal vaccines.
CI  - Copyright (c) 2016 Acta Materialia Inc. Published by Elsevier Ltd. All rights 
      reserved.
FAU - Ghaffar, Khairunnisa Abdul
AU  - Ghaffar KA
AD  - The University of Queensland, School of Chemistry and Molecular Biosciences, 
      Brisbane, QLD 4072, Australia.
FAU - Marasini, Nirmal
AU  - Marasini N
AD  - The University of Queensland, School of Chemistry and Molecular Biosciences, 
      Brisbane, QLD 4072, Australia.
FAU - Giddam, Ashwini Kumar
AU  - Giddam AK
AD  - The University of Queensland, School of Chemistry and Molecular Biosciences, 
      Brisbane, QLD 4072, Australia.
FAU - Batzloff, Michael R
AU  - Batzloff MR
AD  - Institute for Glycomics, Griffith University, Gold Coast 4215, Australia.
FAU - Good, Michael F
AU  - Good MF
AD  - Institute for Glycomics, Griffith University, Gold Coast 4215, Australia.
FAU - Skwarczynski, Mariusz
AU  - Skwarczynski M
AD  - The University of Queensland, School of Chemistry and Molecular Biosciences, 
      Brisbane, QLD 4072, Australia.
FAU - Toth, Istvan
AU  - Toth I
AD  - The University of Queensland, School of Chemistry and Molecular Biosciences, 
      Brisbane, QLD 4072, Australia; The University of Queensland, School of Pharmacy, 
      Brisbane, QLD 4072, Australia; The University of Queensland, Institute for 
      Molecular Biosciences, St Lucia, QLD 4072, Australia. Electronic address: 
      i.toth@uq.edu.au.
LA  - eng
PT  - Journal Article
DEP - 20160407
PL  - England
TA  - Acta Biomater
JT  - Acta biomaterialia
JID - 101233144
RN  - 0 (Immunoglobulin A)
RN  - 0 (Immunoglobulin G)
RN  - 0 (Lipopeptides)
RN  - 0 (Liposomes)
RN  - 0 (Streptococcal Vaccines)
SB  - IM
MH  - Administration, Intranasal
MH  - Amino Acid Sequence
MH  - Animals
MH  - Endocytosis
MH  - Immunoglobulin A/metabolism
MH  - Immunoglobulin G/metabolism
MH  - Lipopeptides/*administration & dosage/chemical synthesis/chemistry/immunology
MH  - Liposomes
MH  - Mice
MH  - Streptococcal Vaccines/*administration & dosage/immunology
MH  - Streptococcus pyogenes/*immunology
OTO - NOTNLM
OT  - Cationic liposomes
OT  - Group A Streptococcus
OT  - Intranasal immunization
OT  - Peptide vaccine
EDAT- 2016/04/12 06:00
MHDA- 2017/10/21 06:00
CRDT- 2016/04/12 06:00
PHST- 2015/12/14 00:00 [received]
PHST- 2016/02/25 00:00 [revised]
PHST- 2016/04/07 00:00 [accepted]
PHST- 2016/04/12 06:00 [entrez]
PHST- 2016/04/12 06:00 [pubmed]
PHST- 2017/10/21 06:00 [medline]
AID - S1742-7061(16)30165-9 [pii]
AID - 10.1016/j.actbio.2016.04.012 [doi]
PST - ppublish
SO  - Acta Biomater. 2016 Sep 1;41:161-8. doi: 10.1016/j.actbio.2016.04.012. Epub 2016 
      Apr 7.