PMID- 27063575
OWN - NLM
STAT- MEDLINE
DCOM- 20170221
LR  - 20220316
IS  - 1095-8355 (Electronic)
IS  - 1065-6995 (Linking)
VI  - 40
IP  - 7
DP  - 2016 Jul
TI  - TGF-beta1 promotes bovine mammary fibroblast proliferation through the ERK 1/2 
      signalling pathway.
PG  - 750-60
LID - 10.1002/cbin.10609 [doi]
AB  - The abnormal proliferation of bovine mammary fibroblasts (BMFBs) impairs mammary 
      gland development and lactation. Severe manifestations develop into breast 
      fibrosis, leading to the culling of cows and causing serious losses to the dairy 
      industry. Transforming growth factor beta1 (TGF-beta1) is an important modulator of 
      cell proliferation and extracellular matrix formation; however, limited 
      information is available on BMFBs. In this study, a convenient and stable culture 
      method for BMFBs was established. Treatment with 5 ng/mL of TGF-beta1 significantly 
      promoted the proliferation of BMFBs and accelerated the cell cycle. TGF-beta1 
      stimulation for up to 12 h significantly increased the relative ERK1/2 mRNA 
      expression and enhanced the protein expression of p-ERK1/2 and cyclin D1. 
      Conversely, the ERK1/2 inhibitor PD98059 blocked these TGF-beta1 effects. Further 
      exploration using a mouse model showed that TGF-beta1 significantly increased the 
      proportion of fibroblasts and accelerating the cell transition from the G1 to 
      G2/M phases. In addition, TGF-beta1 enhanced the expression of fibrosis markers, 
      alpha-SMA and I Collagen, which could be blocked efficiently by the PD98059 in mouse 
      mammary gland. Finally, immunofluorescence analysis confirmed that TGF-beta1 
      promoted fibroblast proliferation in healthy dairy cows after normal long-term 
      dietary corn straw roughage supplementation. It is suggested that the diet may 
      promote mammary fibroblast proliferation by raising the level of TGF-beta1. Our 
      study provides new insights into how nutrition causes undesirable changes in 
      mammary gland structure.
CI  - (c) 2016 International Federation for Cell Biology.
FAU - Gao, Yuanyuan
AU  - Gao Y
AD  - College of Animal Science, Jilin University, Changchun, Jilin, China.
FAU - Wang, Yuping
AU  - Wang Y
AD  - College of Animal Science, Jilin University, Changchun, Jilin, China.
FAU - Li, Yingying
AU  - Li Y
AD  - College of Animal Science, Jilin University, Changchun, Jilin, China.
FAU - Xia, Xiaojing
AU  - Xia X
AD  - College of Veterinary Medicine, Jilin University, Xi'an Road 5333, Changchun, 
      Jilin, China.
FAU - Zhao, Shuang
AU  - Zhao S
AD  - College of Animal Science, Jilin University, Changchun, Jilin, China.
FAU - Che, Yanyi
AU  - Che Y
AD  - College of Veterinary Medicine, Jilin University, Xi'an Road 5333, Changchun, 
      Jilin, China.
FAU - Sun, Yingying
AU  - Sun Y
AD  - College of Veterinary Medicine, Jilin University, Xi'an Road 5333, Changchun, 
      Jilin, China.
FAU - Lei, Liancheng
AU  - Lei L
AD  - College of Veterinary Medicine, Jilin University, Xi'an Road 5333, Changchun, 
      Jilin, China.
LA  - eng
PT  - Journal Article
DEP - 20160505
PL  - England
TA  - Cell Biol Int
JT  - Cell biology international
JID - 9307129
RN  - 0 (Collagen Type I)
RN  - 0 (Transforming Growth Factor beta1)
RN  - 136601-57-5 (Cyclin D1)
SB  - IM
MH  - Animals
MH  - Cattle
MH  - Cattle Diseases/chemically induced/pathology
MH  - Cell Culture Techniques/methods/*veterinary
MH  - Cell Cycle/drug effects
MH  - Cell Division/drug effects
MH  - Cell Proliferation/drug effects
MH  - Collagen Type I/metabolism
MH  - Cyclin D1/metabolism
MH  - Extracellular Matrix/metabolism
MH  - Female
MH  - Fibroblasts/*drug effects/enzymology/metabolism/pathology
MH  - Fibrosis/chemically induced/metabolism/veterinary
MH  - MAP Kinase Signaling System/*drug effects
MH  - Mammary Glands, Animal/*drug effects/metabolism/*pathology
MH  - Mice
MH  - Transforming Growth Factor beta1/metabolism/*pharmacology
OTO - NOTNLM
OT  - ERK 1/2 signalling pathway
OT  - TGF-beta1
OT  - fibroblasts
OT  - proliferation
EDAT- 2016/04/12 06:00
MHDA- 2017/02/22 06:00
CRDT- 2016/04/12 06:00
PHST- 2015/10/30 00:00 [received]
PHST- 2016/04/04 00:00 [accepted]
PHST- 2016/04/12 06:00 [entrez]
PHST- 2016/04/12 06:00 [pubmed]
PHST- 2017/02/22 06:00 [medline]
AID - 10.1002/cbin.10609 [doi]
PST - ppublish
SO  - Cell Biol Int. 2016 Jul;40(7):750-60. doi: 10.1002/cbin.10609. Epub 2016 May 5.