PMID- 27066317
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20160412
LR  - 20210109
IS  - 2190-5991 (Print)
IS  - 2190-6009 (Electronic)
IS  - 2190-5991 (Linking)
VI  - 7
IP  - 1
DP  - 2016 Mar
TI  - Cachexia-associated adipose tissue morphological rearrangement in 
      gastrointestinal cancer patients.
PG  - 37-47
LID - 10.1002/jcsm.12037 [doi]
AB  - BACKGROUND AND AIMS: Cachexia is a syndrome characterized by marked involuntary 
      loss of body weight. Recently, adipose tissue (AT) wasting has been shown to 
      occur before the appearance of other classical cachexia markers. We investigated 
      the composition and rearrangement of the extracellular matrix, adipocyte 
      morphology and inflammation in the subcutaneous AT (scAT) pad of gastrointestinal 
      cancer patients. METHODS: Surgical biopsies for scAT were obtained from 
      gastrointestinal cancer patients, who were signed up into the following groups: 
      cancer cachexia (CC, n = 11), weight-stable cancer (WSC, n = 9) and weight-stable 
      control (non-cancer) (control, n = 7). The stable weight groups were considered 
      as those with no important weight change during the last year and body mass index 
      <25 kg/m(2). Subcutaneous AT fibrosis was quantified and characterized by 
      quantitative PCR, histological analysis and immunohistochemistry. RESULTS: The 
      degree of fibrosis and the distribution and collagen types (I and III) were 
      different in WSC and CC patients. CC patients showed more pronounced fibrosis in 
      comparison with WSC. Infiltrating macrophages surrounding adipocytes and CD3 Ly 
      were found in the fibrotic areas of scAT. Subcutaneous AT fibrotic areas 
      demonstrated increased monocyte chemotactic protein 1 (MCP-1) and Cluster of 
      Differentiation (CD)68 gene expression in cancer patients. CONCLUSIONS: Our data 
      indicate architectural modification consisting of fibrosis and inflammatory cell 
      infiltration in scAT as induced by cachexia in gastrointestinal cancer patients. 
      The latter was characterized by the presence of macrophages and lymphocytes, more 
      evident in the fibrotic areas. In addition, increased MCP-1 and CD68 gene 
      expression in scAT from cancer patients may indicate an important role of these 
      markers in the early phases of cancer.
FAU - Batista, Miguel L Jr
AU  - Batista ML Jr
AD  - Laboratory of Adipose Tissue Biology, Integrated Group of Biotechnology 
      University of Mogi das Cruzes Mogi das Cruzes Brazil; Cancer Metabolism Research 
      Group, Institute of Biomedical Sciences University of Sao Paulo Sao Paulo Brazil.
FAU - Henriques, Felipe S
AU  - Henriques FS
AD  - Laboratory of Adipose Tissue Biology, Integrated Group of Biotechnology 
      University of Mogi das Cruzes Mogi das Cruzes Brazil.
FAU - Neves, Rodrigo X
AU  - Neves RX
AD  - Laboratory of Adipose Tissue Biology, Integrated Group of Biotechnology 
      University of Mogi das Cruzes Mogi das Cruzes Brazil; Cancer Metabolism Research 
      Group, Institute of Biomedical Sciences University of Sao Paulo Sao Paulo Brazil.
FAU - Olivan, Mireia R
AU  - Olivan MR
AD  - Cancer Metabolism Research Group, Institute of Biomedical Sciences University of 
      Sao Paulo Sao Paulo Brazil.
FAU - Matos-Neto, Emidio M
AU  - Matos-Neto EM
AD  - Cancer Metabolism Research Group, Institute of Biomedical Sciences University of 
      Sao Paulo Sao Paulo Brazil.
FAU - Alcantara, Paulo S M
AU  - Alcantara PS
AD  - Department of Clinical Surgery, University Hospital University of Sao Paulo Sao 
      Paulo Brazil.
FAU - Maximiano, Linda F
AU  - Maximiano LF
AD  - Department of Clinical Surgery, University Hospital University of Sao Paulo Sao 
      Paulo Brazil.
FAU - Otoch, Jose P
AU  - Otoch JP
AD  - Department of Clinical Surgery, University Hospital University of Sao Paulo Sao 
      Paulo Brazil.
FAU - Alves, Michele J
AU  - Alves MJ
AD  - Cancer Metabolism Research Group, Institute of Biomedical Sciences University of 
      Sao Paulo Sao Paulo Brazil.
FAU - Seelaender, Marilia
AU  - Seelaender M
AD  - Cancer Metabolism Research Group, Institute of Biomedical Sciences University of 
      Sao Paulo Sao Paulo Brazil.
LA  - eng
PT  - Journal Article
DEP - 20150604
PL  - Germany
TA  - J Cachexia Sarcopenia Muscle
JT  - Journal of cachexia, sarcopenia and muscle
JID - 101552883
PMC - PMC4799865
OTO - NOTNLM
OT  - Adipose tissue
OT  - Extracellular matrix
OT  - Fibrosis, Inflammation, Cachexia
EDAT- 2016/04/12 06:00
MHDA- 2016/04/12 06:01
PMCR- 2016/03/01
CRDT- 2016/04/12 06:00
PHST- 2014/12/19 00:00 [received]
PHST- 2015/03/11 00:00 [revised]
PHST- 2015/03/30 00:00 [accepted]
PHST- 2016/04/12 06:00 [entrez]
PHST- 2016/04/12 06:00 [pubmed]
PHST- 2016/04/12 06:01 [medline]
PHST- 2016/03/01 00:00 [pmc-release]
AID - JCSM12037 [pii]
AID - 10.1002/jcsm.12037 [doi]
PST - ppublish
SO  - J Cachexia Sarcopenia Muscle. 2016 Mar;7(1):37-47. doi: 10.1002/jcsm.12037. Epub 
      2015 Jun 4.