PMID- 27067271
OWN - NLM
STAT- MEDLINE
DCOM- 20170207
LR  - 20191210
IS  - 1651-2251 (Electronic)
IS  - 0001-6489 (Linking)
VI  - 136
IP  - 9
DP  - 2016 Sep
TI  - Droplet digital PCR and qRT-PCR to detect circulating miR-21 in laryngeal 
      squamous cell carcinoma and pre-malignant laryngeal lesions.
PG  - 923-32
LID - 10.3109/00016489.2016.1165862 [doi]
AB  - CONCLUSIONS: The present study indicates that miR-21 is involved in progression 
      from normal to pre-malignant laryngeal lesions (PLLs) and from PLLs to laryngeal 
      squamous cell carcinoma (LSCC). Furthermore, normalized PCR results for miR-21 
      might be used to discriminate between normal and ordinary hyperplasia before the 
      emergence of dysplasias and pre-malignant lesions with malignant potential. 
      OBJECTIVE: To investigate a sensitive marker that contributes to progression from 
      normal tissue to PLLs and from PLLs to LSCC. METHODS: In 116 PLLs and LSCC 
      patients and 19 without dysplasia matched sets of tissue and plasma samples from 
      Beijing Tongren Hospital, miR-21 was analysed by droplet digital PCR and 
      quantitative real-time polymerase chain reaction based on paraffin-embedded 
      tumour tissue and plasma. RESULTS: Compared with controls, miR-21 levels in 
      tissue and plasma were significantly higher for both PLL and LSCC groups (for 
      both groups vs controls: p < 0.0001 for tissue; p < 0.05 for plasma). Areas under 
      receiver-operating curves (AUC) for tissue miR-21 were PLL group vs controls: 
      0.9026 and LSCC group vs controls: 0.8681. For plasma miR-21, AUCs were PLL group 
      vs controls: 0.7355 and LSCC group vs control: 0.6723.
FAU - Wei, Li
AU  - Wei L
AD  - a Department of Pathology , Beijing Tongren Hospital, Capital Medical University, 
      Beijing Key Laboratory of Head and Neck Molecular Diagnostic Pathology , Beijing 
      , PR China ;
FAU - Mao, Meiling
AU  - Mao M
AD  - a Department of Pathology , Beijing Tongren Hospital, Capital Medical University, 
      Beijing Key Laboratory of Head and Neck Molecular Diagnostic Pathology , Beijing 
      , PR China ;
AD  - b Beijing Tsinghua Changgung Hospital , Beijing , PR China.
FAU - Liu, Honggang
AU  - Liu H
AD  - a Department of Pathology , Beijing Tongren Hospital, Capital Medical University, 
      Beijing Key Laboratory of Head and Neck Molecular Diagnostic Pathology , Beijing 
      , PR China ;
LA  - eng
PT  - Comparative Study
PT  - Evaluation Study
PT  - Journal Article
DEP - 20160412
PL  - England
TA  - Acta Otolaryngol
JT  - Acta oto-laryngologica
JID - 0370354
RN  - 0 (Biomarkers, Tumor)
RN  - 0 (MIRN21 microRNA, human)
RN  - 0 (MicroRNAs)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Biomarkers, Tumor/blood
MH  - Carcinoma, Squamous Cell/*blood/diagnosis/pathology
MH  - Feasibility Studies
MH  - Female
MH  - Humans
MH  - Laryngeal Neoplasms/*blood/diagnosis/pathology
MH  - Larynx/pathology
MH  - Male
MH  - MicroRNAs/*blood
MH  - Middle Aged
MH  - Polymerase Chain Reaction/methods
MH  - Precancerous Conditions/*blood/diagnosis/pathology
MH  - Prospective Studies
MH  - ROC Curve
MH  - Young Adult
OTO - NOTNLM
OT  - Pre-malignant laryngeal lesion
OT  - droplet digital PCR
OT  - laryngeal squamous cell carcinoma
OT  - miRNA-21
EDAT- 2016/04/14 06:00
MHDA- 2017/02/09 06:00
CRDT- 2016/04/13 06:00
PHST- 2016/04/13 06:00 [entrez]
PHST- 2016/04/14 06:00 [pubmed]
PHST- 2017/02/09 06:00 [medline]
AID - 10.3109/00016489.2016.1165862 [doi]
PST - ppublish
SO  - Acta Otolaryngol. 2016 Sep;136(9):923-32. doi: 10.3109/00016489.2016.1165862. 
      Epub 2016 Apr 12.