PMID- 27069535
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20160412
LR  - 20201001
IS  - 1943-8141 (Print)
IS  - 1943-8141 (Electronic)
IS  - 1943-8141 (Linking)
VI  - 8
IP  - 1
DP  - 2016
TI  - miR-144 suppresses the growth and metastasis of laryngeal squamous cell carcinoma 
      by targeting IRS1.
PG  - 1-11
AB  - Increasing evidence has been suggested that microRNA-144 (miR-144) involved in 
      tumor initiation, development and metastasis in various cancers. However, the 
      biological roles and potential mechanisms of miR-144 in laryngeal squamous cell 
      carcinoma (LSCC) remain unclear. In the present study, we discovered that miR-144 
      expression levels in LSCC tissues were significantly lower than those of adjacent 
      normal tissues. Furthermore, overexpression of miR-144 in LSCC cells inhibited 
      cell proliferation, colony formation, migration, and invasion in vitro. 
      Consistently, stable overexpression of miR-144 suppressed the growth of LSCC cell 
      xenografts in vivo. Bioinformatic algorithms and luciferase reporter assays 
      confirmed that insulin receptor substrate 1 (IRS1) is a direct target of miR-144. 
      Overexpreesion of miR-144 obviously decreased IRS1 expression thereby suppressing 
      phosphatidylinositol 3-kinase (PI3K)/AKT pathway activation. Further functional 
      studies suggested that downregulation of IRS1 had similar effects as that of 
      miR-144 overexpression, and upregulation of IRS1 partially reversed the 
      inhibitory effects of miR-144. These findings suggested that miR-144 functioned 
      as a tumor suppressor in LSCC by targeting IRS1, and that miR-144 might serve as 
      a potential target for LSCC treatment.
FAU - Wu, Xin
AU  - Wu X
AD  - Department of Otorhinolaryngology-Head and Neck Surgery, The First Hospital of 
      Jilin University 3302 Jilin Main Road, Changchun 130021, China.
FAU - Cui, Chang-Lei
AU  - Cui CL
AD  - Department of Anesthesiology, The First Hospital of Jilin University 3302 Jilin 
      Main Road, Changchun 130021, China.
FAU - Chen, Wei-Lun
AU  - Chen WL
AD  - Department of Otorhinolaryngology-Head and Neck Surgery, The First Hospital of 
      Jilin University 3302 Jilin Main Road, Changchun 130021, China.
FAU - Fu, Zhong-Ying
AU  - Fu ZY
AD  - Department of Otorhinolaryngology-Head and Neck Surgery, The First Hospital of 
      Jilin University 3302 Jilin Main Road, Changchun 130021, China.
FAU - Cui, Xiang-Yan
AU  - Cui XY
AD  - Department of Otorhinolaryngology-Head and Neck Surgery, The First Hospital of 
      Jilin University 3302 Jilin Main Road, Changchun 130021, China.
FAU - Gong, Xu
AU  - Gong X
AD  - Department of Hand Surgery, The First Hospital of Jilin University 3302 Jilin 
      Main Road, Changchun 130021, China.
LA  - eng
PT  - Journal Article
DEP - 20160115
PL  - United States
TA  - Am J Transl Res
JT  - American journal of translational research
JID - 101493030
PMC - PMC4759411
OTO - NOTNLM
OT  - IRS1
OT  - growth
OT  - laryngeal squamous cell carcinoma
OT  - metastasis
OT  - miR-144
EDAT- 2016/04/14 06:00
MHDA- 2016/04/14 06:01
PMCR- 2016/01/15
CRDT- 2016/04/13 06:00
PHST- 2015/11/12 00:00 [received]
PHST- 2015/12/15 00:00 [accepted]
PHST- 2016/04/13 06:00 [entrez]
PHST- 2016/04/14 06:00 [pubmed]
PHST- 2016/04/14 06:01 [medline]
PHST- 2016/01/15 00:00 [pmc-release]
PST - epublish
SO  - Am J Transl Res. 2016 Jan 15;8(1):1-11. eCollection 2016.