PMID- 27069754
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20160412
LR  - 20201001
IS  - 2160-1992 (Print)
IS  - 2160-1992 (Electronic)
IS  - 2160-1992 (Linking)
VI  - 5
IP  - 2
DP  - 2015
TI  - Fixed nuclei as alternative template of BIOMED-2 multiplex polymerase chain 
      reaction for immunoglobulin gene clonality testing in B-cell malignancies.
PG  - 62-78
AB  - Evaluation of immunoglobulin (Ig) gene rearrangements with BIOMED-2 multiplex PCR 
      has become a standard detection of clonality in mature B cell malignancies. 
      Conventionally, this method is relatively labor-intensive and time-consuming, as 
      it requires DNA isolation from bone marrow aspirates (BM) or peripheral blood 
      (PB) in patients with BM or PB involvement. On the other hand, fluorescence in 
      situ hybridization (FISH) is routinely used as genetic screening in B cell 
      malignancies, but the surplus fixed nuclei initially prepared for FISH usually 
      turn useless afterwards. We sought to use these surplus nuclei after FISH as a 
      template to perform PCR-based Ig gene clonality testing. Templates of 12 patients 
      with mature B cell malignancies, which consisted of both DNA isolated with 
      commercial DNA isolation kit from fresh BM or PB (DNA group) and the fixed nuclei 
      initially prepared for FISH (nuclei group) from the same individuals, were 
      subjected to PCR with BIOMED-2 primer sets for immunoglobulin heavy chain and 
      kappa light chain under recommended conditions. Our result, for the first time, 
      showed a high consistency between the two groups in detecting B cell clonality, 
      which indicates that nuclei for FISH can function as a reliable template 
      comparable to fresh tissue-isolated DNA in PCR based Ig clonality testing. This 
      offers a simple, rapid and more economical alternative to standard Ig testing 
      based on regular DNA.
FAU - Tang, Yuan
AU  - Tang Y
AD  - Department of Pathology, West China Hospital, Sichuan University China.
FAU - Chen, Jie
AU  - Chen J
AD  - Department of Pathology, West China Hospital, Sichuan University China.
FAU - Wang, Jianchao
AU  - Wang J
AD  - Department of Pathology, West China Hospital, Sichuan University China.
FAU - Zheng, Ke
AU  - Zheng K
AD  - Department of Pathology, West China Hospital, Sichuan University China.
FAU - Liao, Dianying
AU  - Liao D
AD  - Department of Pathology, West China Hospital, Sichuan University China.
FAU - Liao, Xiaomei
AU  - Liao X
AD  - Department of Hematology, West China Hospital, Sichuan University China.
FAU - Liu, Weiping
AU  - Liu W
AD  - Department of Pathology, West China Hospital, Sichuan University China.
FAU - Wang, Lin
AU  - Wang L
AD  - Department of Pathology, West China Hospital, Sichuan University China.
LA  - eng
PT  - Journal Article
DEP - 20151225
PL  - United States
TA  - Am J Blood Res
JT  - American journal of blood research
JID - 101569577
PMC - PMC4769348
OTO - NOTNLM
OT  - BIOMED-2 PCR
OT  - Carnoy's fixative
OT  - FISH
OT  - Fixed nuclei
OT  - immunoglobulin (Ig) clonality testing
EDAT- 2015/01/01 00:00
MHDA- 2015/01/01 00:01
PMCR- 2015/12/25
CRDT- 2016/04/13 06:00
PHST- 2015/10/01 00:00 [received]
PHST- 2015/11/09 00:00 [accepted]
PHST- 2016/04/13 06:00 [entrez]
PHST- 2015/01/01 00:00 [pubmed]
PHST- 2015/01/01 00:01 [medline]
PHST- 2015/12/25 00:00 [pmc-release]
PST - epublish
SO  - Am J Blood Res. 2015 Dec 25;5(2):62-78. eCollection 2015.