PMID- 27069766
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20160412
LR  - 20240324
IS  - 2160-8407 (Print)
IS  - 2160-8407 (Electronic)
VI  - 6
IP  - 1
DP  - 2016
TI  - (68)Ga-DOTATOC PET and gene expression profile in patients with neuroendocrine 
      carcinomas: strong correlation between PET tracer uptake and gene expression of 
      somatostatin receptor subtype 2.
PG  - 59-72
AB  - Somatostatin receptor expression on both protein and gene expression level was 
      compared with in vivo (68)Ga-DOTATOC PET/CT in patients with neuroendocrine 
      carcinomas (NEC). Twenty-one patients with verified NEC who underwent a 
      (68)Ga-DOTATOC PET/CT between November 2012 and May 2014, were retrospectively 
      included. By real-time polymerase chain reaction, we quantitatively determined 
      the gene expression of several genes and compared with (68)Ga-DOTATOC PET uptake. 
      By immunohistochemistry we qualitatively studied the expression of assorted 
      proteins in NEC. The median age at diagnosis was 68 years (range 41-84) years. 
      All patients had WHO performance status 0-1. Median Ki67 index was 50% (range 
      20-100%). Gene expression of somatostatin receptor subtype (SSTR) 2 and Ki67 were 
      both positively correlated to the (68)Ga-DOTATOC uptake (r=0.89; p<0.0001 and 
      r=0.5; p=0.021, respectively). Furthermore, SSTR2 and SSTR5 gene expression were 
      strongly and positively correlated (r=0.57; p=0.006). This study as the first 
      verifies a positive and close correlation of (68)Ga-DOTATOC uptake and gene 
      expression of SSTR2 in NEC. SSTR2 gene expression has a stronger correlation to 
      (68)Ga-DOTATOC uptake than SSTR5. In addition, the results indicate that the gene 
      expression levels of SSTR2 and SSTR5 at large follow one another.
FAU - Olsen, Ingrid H
AU  - Olsen IH
AD  - European NET Centre of Excellence, Rigshospitalet, University of 
      CopenhagenDenmark; Department of Clinical Physiology, Nuclear Medicine & PET and 
      Cluster for Molecular Imaging, Rigshospitalet and University of 
      CopenhagenDenmark; Department of Endocrinology, Rigshospitalet, University of 
      CopenhagenDenmark; Department of Surgical Gastroenterology, Rigshospitalet, 
      University of CopenhagenDenmark; Department of Oncology, Rigshospitalet, 
      University of CopenhagenDenmark; Department of Pathology, Rigshospitalet, 
      University of CopenhagenDenmark.
FAU - Langer, Seppo W
AU  - Langer SW
AD  - European NET Centre of Excellence, Rigshospitalet, University of 
      CopenhagenDenmark; Department of Oncology, Rigshospitalet, University of 
      CopenhagenDenmark.
FAU - Federspiel, Birgitte H
AU  - Federspiel BH
AD  - European NET Centre of Excellence, Rigshospitalet, University of 
      CopenhagenDenmark; Department of Pathology, Rigshospitalet, University of 
      CopenhagenDenmark.
FAU - Oxbol, Jytte
AU  - Oxbol J
AD  - European NET Centre of Excellence, Rigshospitalet, University of 
      CopenhagenDenmark; Department of Clinical Physiology, Nuclear Medicine & PET and 
      Cluster for Molecular Imaging, Rigshospitalet and University of 
      CopenhagenDenmark.
FAU - Loft, Annika
AU  - Loft A
AD  - European NET Centre of Excellence, Rigshospitalet, University of 
      CopenhagenDenmark; Department of Clinical Physiology, Nuclear Medicine & PET and 
      Cluster for Molecular Imaging, Rigshospitalet and University of 
      CopenhagenDenmark.
FAU - Berthelsen, Anne Kiil
AU  - Berthelsen AK
AD  - European NET Centre of Excellence, Rigshospitalet, University of 
      CopenhagenDenmark; Department of Clinical Physiology, Nuclear Medicine & PET and 
      Cluster for Molecular Imaging, Rigshospitalet and University of 
      CopenhagenDenmark.
FAU - Mortensen, Jann
AU  - Mortensen J
AD  - European NET Centre of Excellence, Rigshospitalet, University of 
      CopenhagenDenmark; Department of Clinical Physiology, Nuclear Medicine & PET and 
      Cluster for Molecular Imaging, Rigshospitalet and University of 
      CopenhagenDenmark.
FAU - Oturai, Peter
AU  - Oturai P
AD  - European NET Centre of Excellence, Rigshospitalet, University of 
      CopenhagenDenmark; Department of Clinical Physiology, Nuclear Medicine & PET and 
      Cluster for Molecular Imaging, Rigshospitalet and University of 
      CopenhagenDenmark.
FAU - Knigge, Ulrich
AU  - Knigge U
AD  - European NET Centre of Excellence, Rigshospitalet, University of 
      CopenhagenDenmark; Department of Clinical Physiology, Nuclear Medicine & PET and 
      Cluster for Molecular Imaging, Rigshospitalet and University of 
      CopenhagenDenmark; Department of Endocrinology, Rigshospitalet, University of 
      CopenhagenDenmark; Department of Surgical Gastroenterology, Rigshospitalet, 
      University of CopenhagenDenmark.
FAU - Kjaer, Andreas
AU  - Kjaer A
AD  - European NET Centre of Excellence, Rigshospitalet, University of 
      CopenhagenDenmark; Department of Clinical Physiology, Nuclear Medicine & PET and 
      Cluster for Molecular Imaging, Rigshospitalet and University of 
      CopenhagenDenmark.
LA  - eng
PT  - Journal Article
DEP - 20160128
PL  - United States
TA  - Am J Nucl Med Mol Imaging
JT  - American journal of nuclear medicine and molecular imaging
JID - 101564121
PMC - PMC4749505
OTO - NOTNLM
OT  - 68Ga-DOTATOC PET/CT
OT  - Neuroendocrine carcinoma
OT  - gene expression
OT  - imaging
OT  - immunohistochemistry
OT  - mammalian target of rapamycin
OT  - neuroendocrine tumors
OT  - somatostatin receptor type 2
OT  - somatostatin receptor type 5
OT  - urokinase-type plasminogen activator receptor
EDAT- 2016/04/14 06:00
MHDA- 2016/04/14 06:01
PMCR- 2016/01/28
CRDT- 2016/04/13 06:00
PHST- 2015/09/24 00:00 [received]
PHST- 2015/12/04 00:00 [accepted]
PHST- 2016/04/13 06:00 [entrez]
PHST- 2016/04/14 06:00 [pubmed]
PHST- 2016/04/14 06:01 [medline]
PHST- 2016/01/28 00:00 [pmc-release]
PST - epublish
SO  - Am J Nucl Med Mol Imaging. 2016 Jan 28;6(1):59-72. eCollection 2016.