PMID- 27070750
OWN - NLM
STAT- MEDLINE
DCOM- 20170330
LR  - 20200220
IS  - 1802-9973 (Electronic)
IS  - 0862-8408 (Linking)
VI  - 65
IP  - 3
DP  - 2016 Jul 18
TI  - Plasma concentrations and subcutaneous adipose tissue mRNA expression of 
      clusterin in obesity and type 2 diabetes mellitus: the effect of short-term 
      hyperinsulinemia, very-low-calorie diet and bariatric surgery.
PG  - 481-92
AB  - Clusterin is a heterodimeric glycoprotein with wide range of functions. To 
      further explore its possible regulatory role in energy homeostasis and in adipose 
      tissue, we measured plasma clusterin and its mRNA expression in subcutaneous 
      adipose tissue (SCAT) of 15 healthy lean women, 15 obese women (OB) and 15 obese 
      women with type 2 diabetes mellitus (T2DM) who underwent a 2-week very 
      low-calorie diet (VLCD), 10 obese women without T2DM who underwent laparoscopic 
      sleeve gastrectomy (LSG) and 8 patients with T2DM, 8 patients with impaired 
      glucose tolerance (IGT) and 8 normoglycemic patients who underwent 
      hyperinsulinemic euglycemic clamp (HEC). VLCD decreased plasma clusterin in OB 
      but not in T2DM patients while LSG and HEC had no effect. Clusterin mRNA 
      expression in SCAT at baseline was increased in OB and T2DM patients compared 
      with controls. Clusterin mRNA expression decreased 6 months after LSG and 
      remained decreased 12 months after LSG. mRNA expression of clusterin was elevated 
      at the end of HEC compared with baseline only in normoglycemic but not in IGT or 
      T2DM patients. In summary, our data suggest a possible local regulatory role for 
      clusterin in the adipose tissue rather than its systemic involvement in the 
      regulation of energy homeostasis.
FAU - Klouckova, J
AU  - Klouckova J
AD  - Institute of Medical Biochemistry and Laboratory Diagnostics, First Faculty of 
      Medicine, Charles University and General University Hospital, Prague, Czech 
      Republic, Department of Obesitology, Institute of Endocrinology, Prague, Czech 
      Republic. mhalu@lf1.cuni.cz.
FAU - Lacinova, Z
AU  - Lacinova Z
FAU - Kavalkova, P
AU  - Kavalkova P
FAU - Trachta, P
AU  - Trachta P
FAU - Kasalicky, M
AU  - Kasalicky M
FAU - Haluzikova, D
AU  - Haluzikova D
FAU - Mraz, M
AU  - Mraz M
FAU - Haluzik, M
AU  - Haluzik M
LA  - eng
PT  - Journal Article
DEP - 20160412
PL  - Czech Republic
TA  - Physiol Res
JT  - Physiological research
JID - 9112413
RN  - 0 (Clusterin)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - *Bariatric Surgery
MH  - *Caloric Restriction
MH  - Case-Control Studies
MH  - Clusterin/*blood
MH  - Diabetes Mellitus, Type 2/*blood/complications
MH  - Energy Metabolism
MH  - Female
MH  - Humans
MH  - Middle Aged
MH  - Obesity/*blood/complications/therapy
MH  - Protein Array Analysis
MH  - RNA, Messenger/metabolism
MH  - Subcutaneous Fat/*metabolism
EDAT- 2016/04/14 06:00
MHDA- 2017/03/31 06:00
CRDT- 2016/04/13 06:00
PHST- 2016/04/13 06:00 [entrez]
PHST- 2016/04/14 06:00 [pubmed]
PHST- 2017/03/31 06:00 [medline]
AID - 933121 [pii]
AID - 10.33549/physiolres.933121 [doi]
PST - ppublish
SO  - Physiol Res. 2016 Jul 18;65(3):481-92. doi: 10.33549/physiolres.933121. Epub 2016 
      Apr 12.