PMID- 27073452
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20211130
IS  - 1792-0981 (Print)
IS  - 1792-1015 (Electronic)
IS  - 1792-0981 (Linking)
VI  - 11
IP  - 4
DP  - 2016 Apr
TI  - CEP3 and CEP17 DNA probe potential in the genetic diagnosis and prognostic 
      prediction of esophageal squamous cell cancer.
PG  - 1375-1380
AB  - The aim of the present study was to investigate the clinical application of 
      molecular pathological diagnosis for the prognosis of Kazakh patients with 
      esophageal squamous cell carcinoma (ESCC) using centromere enumeration probes 
      (CEPs) for chromosomes 3 and 17. A total of 40 patients with ESCC that had 
      received radical surgical treatment and 10 healthy control participants were 
      enrolled in the present study. Touch preparations of fresh cancerous and normal 
      tissues were completed and fluorescence in situ hybridization (FISH) was 
      performed to count the copy numbers of CEP 3 and 17, and abnormalities were 
      analyzed, in comparison with routine pathological diagnoses. FISH analysis 
      demonstrated that abnormal copy numbers of CEP 3 and 17 (aneuploidy) were 
      detected in all 40 patients with ESCC. CEP 3 and 17 polyploidy rates differed 
      significantly between poorly differentiated, moderately differentiated and 
      well-differentiated ESCC groups (P<0.05): Well-differentiated, 35.38 and 30.92%; 
      moderately differentiated, 55.81 and 44.43%; and poorly differentiated, 76.26 and 
      71.90%, respectively. Furthermore, polyploidy rates were significantly increased 
      in the group with lymph node metastasis, as compared with the group without (CEP 
      3, P=0.0001; CEP 17, P=0.012). Variations in the copy numbers of CEP 3 and 17 
      were demonstrated to be correlated with the level of differentiation and lymph 
      node metastasis in patients with ESCC. Therefore, the present results indicate 
      that DNA probes may be used to predict prognostic factors in patients with ESCC. 
      Furthermore, FISH technology is an objective and qualitative method that is 
      capable of detecting variations in CEP 3 and 17; therefore, FISH may be used in 
      the genetic diagnosis of ESCC in Kazakh patients.
FAU - Niyaz, Madiniyat
AU  - Niyaz M
AD  - Xinjiang Esophageal Cancer Research Institute, Medical Research Center, First 
      Affiliated Hospital of Xinjiang Medical University, Urumqi, Xinjiang 830054, P.R. 
      China.
FAU - Abdurahman, Ablajan
AU  - Abdurahman A
AD  - Department of Thoracic Surgery, First Affiliated Hospital of Xinjiang Medical 
      University, Urumqi, Xinjiang 830054, P.R. China.
FAU - Turghun, Abdugheni
AU  - Turghun A
AD  - Department of Thoracic Surgery, First Affiliated Hospital of Xinjiang Medical 
      University, Urumqi, Xinjiang 830054, P.R. China.
FAU - Awut, Idiris
AU  - Awut I
AD  - Department of Thoracic Surgery, First Affiliated Hospital of Xinjiang Medical 
      University, Urumqi, Xinjiang 830054, P.R. China.
LA  - eng
PT  - Journal Article
DEP - 20160216
PL  - Greece
TA  - Exp Ther Med
JT  - Experimental and therapeutic medicine
JID - 101531947
PMC - PMC4812153
OTO - NOTNLM
OT  - DNA probe
OT  - Kazakh patients
OT  - aneuploid
OT  - esophageal cancer
OT  - fluorescence in situ hybridization
EDAT- 2016/04/14 06:00
MHDA- 2016/04/14 06:01
PMCR- 2016/02/16
CRDT- 2016/04/14 06:00
PHST- 2014/11/10 00:00 [received]
PHST- 2015/11/25 00:00 [accepted]
PHST- 2016/04/14 06:00 [entrez]
PHST- 2016/04/14 06:00 [pubmed]
PHST- 2016/04/14 06:01 [medline]
PHST- 2016/02/16 00:00 [pmc-release]
AID - ETM-0-0-3080 [pii]
AID - 10.3892/etm.2016.3080 [doi]
PST - ppublish
SO  - Exp Ther Med. 2016 Apr;11(4):1375-1380. doi: 10.3892/etm.2016.3080. Epub 2016 Feb 
      16.