PMID- 27074878
OWN - NLM
STAT- MEDLINE
DCOM- 20180501
LR  - 20211025
IS  - 1932-7005 (Electronic)
IS  - 1932-6254 (Linking)
VI  - 11
IP  - 8
DP  - 2017 Aug
TI  - Combined treatment with platelet-rich plasma and insulin favours chondrogenic and 
      osteogenic differentiation of human adipose-derived stem cells in 
      three-dimensional collagen scaffolds.
PG  - 2398-2410
LID - 10.1002/term.2139 [doi]
AB  - Osteochondral lesions due to injury or other pathology commonly result in the 
      development of osteoarthritis and progressive joint destruction. Bioengineered 
      scaffolds are widely studied for regenerative surgery strategies in osteochondral 
      defect management, also combining the use of stem cells, growth factors and 
      hormones. The utility in tissue engineering of human adipose-derived stem cells 
      (ASCs) isolated from adipose tissue has been widely noted. Autologous 
      platelet-rich plasma (PRP) represents an alternative strategy in regenerative 
      medicine for the local release of endogenous growth factors and hormones. Here we 
      compared the effects of three-dimensional (3D) collagen type I scaffold culture 
      and combined treatment with PRP and human recombinant insulin on the 
      chondro-/osteogenic differentiation of ASCs. Histochemical and biomolecular 
      analyses demonstrated that chondro-/osteogenic differentiation was increased in 
      ASC-populated 3D collagen scaffolds compared with two-dimensional (2D) plastic 
      dish culture. Chondro-/osteogenic differentiation was further enhanced in the 
      presence of combined PRP (5% v/v) and insulin (100 nm) treatment. In addition, 
      chondro-/osteogenic differentiation associated with the contraction of 
      ASC-populated 3D collagen scaffold and increased beta1/beta3-integrin expression. 
      Inhibition studies demonstrated that PRP/insulin-induced chondro-/osteogenic 
      differentiation is independent of insulin-like growth factor 1 receptor (IGF-1R) 
      and mammalian target of rapamycin (mTOR) signalling; IGF-R1/mTOR inhibition even 
      enhanced ASC chondro-/osteogenic differentiation. Our findings underline that 3D 
      collagen scaffold culture in association with platelet-derived growth factors and 
      insulin favour the chondro-/osteogenic differentiation of ASCs, suggesting new 
      translational applications in regenerative medicine for the management of 
      osteochondral defects. Copyright (c) 2016 John Wiley & Sons, Ltd.
CI  - Copyright (c) 2016 John Wiley & Sons, Ltd.
FAU - Scioli, Maria Giovanna
AU  - Scioli MG
AD  - Institute of Anatomical Pathology, Department of Biomedicine and Prevention, Tor 
      Vergata University, Rome, Italy.
FAU - Bielli, Alessandra
AU  - Bielli A
AD  - Institute of Anatomical Pathology, Department of Biomedicine and Prevention, Tor 
      Vergata University, Rome, Italy.
FAU - Gentile, Pietro
AU  - Gentile P
AD  - Plastic and Reconstructive Surgery, Department of Biomedicine and Prevention, Tor 
      Vergata University, Rome, Italy.
FAU - Cervelli, Valerio
AU  - Cervelli V
AD  - Plastic and Reconstructive Surgery, Department of Biomedicine and Prevention, Tor 
      Vergata University, Rome, Italy.
FAU - Orlandi, Augusto
AU  - Orlandi A
AD  - Institute of Anatomical Pathology, Department of Biomedicine and Prevention, Tor 
      Vergata University, Rome, Italy.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20160414
PL  - England
TA  - J Tissue Eng Regen Med
JT  - Journal of tissue engineering and regenerative medicine
JID - 101308490
RN  - 0 (Insulin)
RN  - 9007-34-5 (Collagen)
SB  - IM
MH  - Adipose Tissue/cytology/*metabolism
MH  - Cell Differentiation/*drug effects
MH  - Cells, Cultured
MH  - Chondrogenesis/*drug effects
MH  - Collagen/*chemistry
MH  - Humans
MH  - Insulin/chemistry/*pharmacology
MH  - Osteogenesis/*drug effects
MH  - Platelet-Rich Plasma/*chemistry
MH  - Stem Cells/cytology/*metabolism
MH  - Tissue Scaffolds/*chemistry
OTO - NOTNLM
OT  - IGF-1R/mTOR signalling
OT  - chondrogenesis/osteogenesis
OT  - collagen gel scaffold
OT  - human adipose-derived stem cells
OT  - insulin
OT  - platelet-rich plasma
EDAT- 2016/04/15 06:00
MHDA- 2018/05/02 06:00
CRDT- 2016/04/15 06:00
PHST- 2015/06/26 00:00 [received]
PHST- 2015/11/18 00:00 [revised]
PHST- 2015/12/10 00:00 [accepted]
PHST- 2016/04/15 06:00 [pubmed]
PHST- 2018/05/02 06:00 [medline]
PHST- 2016/04/15 06:00 [entrez]
AID - 10.1002/term.2139 [doi]
PST - ppublish
SO  - J Tissue Eng Regen Med. 2017 Aug;11(8):2398-2410. doi: 10.1002/term.2139. Epub 
      2016 Apr 14.