PMID- 27075778 OWN - NLM STAT- MEDLINE DCOM- 20170301 LR - 20181113 IS - 2045-2322 (Electronic) IS - 2045-2322 (Linking) VI - 6 DP - 2016 Apr 14 TI - A Fluorescence Approach to Assess the Production of Soluble Microbial Products from Aerobic Granular Sludge Under the Stress of 2,4-Dichlorophenol. PG - 24444 LID - 10.1038/srep24444 [doi] LID - 24444 AB - In this study, a fluorescence approach was used to evaluate the production of soluble microbial products (SMP) in aerobic granular sludge system under the stress of 2,4-dichlorophenol (2,4-DCP). A combined use of three-dimension excitation emission matrix fluorescence spectroscopy (3D-EEM), Parallel factor analysis (PARAFAC), synchronous fluorescence and two-dimensional correlation spectroscopy (2D-COS) were explored to respect the SMP formation in the exposure of different doses of 2,4-DCP. Data implied that the presence of 2,4-DCP had an obvious inhibition on biological nitrogen removal. According to EEM-PARAFAC, two fluorescent components were derived and represented to the presence of fulvic-like substances and humic-like substances in Component 1 and protein-like substances in Component 2. It was found from synchronous fluorescence that protein-like peak presented slightly higher intensity than that of fulvic-like peak. 2D-COS further revealed that fluorescence change took place sequentially in the following order: protein-like fraction > fulvic-like fraction. The obtained results could provide a potential application of fluorescence spectra in the released SMP assessment in the exposure of toxic compound during wastewater treatment. FAU - Wei, Dong AU - Wei D AD - School of Resources and Environment, University of Jinan, Jinan 250022, PR China. FAU - Dong, Heng AU - Dong H AD - School of Resources and Environment, University of Jinan, Jinan 250022, PR China. FAU - Wu, Na AU - Wu N AD - School of Resources and Environment, University of Jinan, Jinan 250022, PR China. FAU - Ngo, Huu Hao AU - Ngo HH AD - School of Civil and Environmental Engineering, University of Technology Sydney, Broadway, NSW 2007, Australia. FAU - Guo, Wenshan AU - Guo W AD - School of Civil and Environmental Engineering, University of Technology Sydney, Broadway, NSW 2007, Australia. FAU - Du, Bin AU - Du B AD - School of Resources and Environment, University of Jinan, Jinan 250022, PR China. FAU - Wei, Qin AU - Wei Q AD - Key Laboratory of Chemical Sensing &Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, PR China. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20160414 PL - England TA - Sci Rep JT - Scientific reports JID - 101563288 RN - 0 (Biological Products) RN - 0 (Chlorophenols) RN - 0 (Sewage) RN - 0 (Water Pollutants, Chemical) RN - N762921K75 (Nitrogen) RN - R669TG1950 (2,4-dichlorophenol) SB - IM MH - Aerobiosis MH - Biological Products/*analysis MH - Chlorophenols/*toxicity MH - Microbial Consortia/*drug effects MH - Nitrogen/metabolism MH - Sewage/*microbiology MH - Spectrometry, Fluorescence/*methods MH - Water Pollutants, Chemical/*toxicity PMC - PMC4830994 EDAT- 2016/04/15 06:00 MHDA- 2017/03/03 06:00 PMCR- 2016/04/14 CRDT- 2016/04/15 06:00 PHST- 2015/12/29 00:00 [received] PHST- 2016/03/22 00:00 [accepted] PHST- 2016/04/15 06:00 [entrez] PHST- 2016/04/15 06:00 [pubmed] PHST- 2017/03/03 06:00 [medline] PHST- 2016/04/14 00:00 [pmc-release] AID - srep24444 [pii] AID - 10.1038/srep24444 [doi] PST - epublish SO - Sci Rep. 2016 Apr 14;6:24444. doi: 10.1038/srep24444.