PMID- 27077111 OWN - NLM STAT- MEDLINE DCOM- 20161230 LR - 20181113 IS - 2352-3964 (Electronic) IS - 2352-3964 (Linking) VI - 5 DP - 2016 Mar TI - Functional Specialty of CD40 and Dendritic Cell Surface Lectins for Exogenous Antigen Presentation to CD8(+) and CD4(+) T Cells. PG - 46-58 LID - 10.1016/j.ebiom.2016.01.029 [doi] AB - Dendritic cells (DCs) are major antigen-presenting cells that can efficiently prime and cross-prime antigen-specific T cells. Delivering antigen to DCs via surface receptors is thus an appealing strategy to evoke cellular immunity. Nonetheless, which DC surface receptor to target to yield the optimal CD8(+) and CD4(+) T cell responses remains elusive. Herein, we report the superiority of CD40 over 9 different lectins and scavenger receptors at evoking antigen-specific CD8(+) T cell responses. However, lectins (e.g., LOX-1 and Dectin-1) were more efficient than CD40 at eliciting CD4(+) T cell responses. Common and distinct patterns of subcellular and intracellular localization of receptor-bound alphaCD40, alphaLOX-1 and alphaDectin-1 further support their functional specialization at enhancing antigen presentation to either CD8(+) or CD4(+) T cells. Lastly, we demonstrate that antigen targeting to CD40 can evoke potent antigen-specific CD8(+) T cell responses in human CD40 transgenic mice. This study provides fundamental information for the rational design of vaccines against cancers and viral infections. FAU - Yin, Wenjie AU - Yin W AD - Baylor Institute for Immunology Research, 3434 Live Oak Street, Dallas, TX 75204, USA; Institute of Biomedical Studies, Baylor University, South 5th Street, Waco, TX 76706, USA. FAU - Gorvel, Laurent AU - Gorvel L AD - Unite de Recherche sur les Maladies Infectieuses, Tropicales Emergentes, IRD 198, CNRS UMR7278, INSERM U1095, 27 bd Jean Moulin, 13385 Cedex 05, Marseille, France. FAU - Zurawski, Sandra AU - Zurawski S AD - Baylor Institute for Immunology Research, 3434 Live Oak Street, Dallas, TX 75204, USA. FAU - Li, Dapeng AU - Li D AD - Baylor Institute for Immunology Research, 3434 Live Oak Street, Dallas, TX 75204, USA. FAU - Ni, Ling AU - Ni L AD - Baylor Institute for Immunology Research, 3434 Live Oak Street, Dallas, TX 75204, USA. FAU - Duluc, Dorothee AU - Duluc D AD - Baylor Institute for Immunology Research, 3434 Live Oak Street, Dallas, TX 75204, USA. FAU - Upchurch, Katherine AU - Upchurch K AD - Baylor Institute for Immunology Research, 3434 Live Oak Street, Dallas, TX 75204, USA; Institute of Biomedical Studies, Baylor University, South 5th Street, Waco, TX 76706, USA. FAU - Kim, JongRok AU - Kim J AD - Baylor Institute for Immunology Research, 3434 Live Oak Street, Dallas, TX 75204, USA. FAU - Gu, Chao AU - Gu C AD - Baylor Institute for Immunology Research, 3434 Live Oak Street, Dallas, TX 75204, USA; Institute of Biomedical Studies, Baylor University, South 5th Street, Waco, TX 76706, USA. FAU - Ouedraogo, Richard AU - Ouedraogo R AD - Baylor Institute for Immunology Research, 3434 Live Oak Street, Dallas, TX 75204, USA. FAU - Wang, Zhiqing AU - Wang Z AD - Baylor Institute for Immunology Research, 3434 Live Oak Street, Dallas, TX 75204, USA. FAU - Xue, Yaming AU - Xue Y AD - Baylor Institute for Immunology Research, 3434 Live Oak Street, Dallas, TX 75204, USA. FAU - Joo, HyeMee AU - Joo H AD - Baylor Institute for Immunology Research, 3434 Live Oak Street, Dallas, TX 75204, USA; Institute of Biomedical Studies, Baylor University, South 5th Street, Waco, TX 76706, USA. FAU - Gorvel, Jean-Pierre AU - Gorvel JP AD - CIML, Aix Marseille Universite, CNRS UMR 7280, INSERM U1104, parc scientifique de Luminy, Case 906, Marseille, France. FAU - Zurawski, Gerard AU - Zurawski G AD - Baylor Institute for Immunology Research, 3434 Live Oak Street, Dallas, TX 75204, USA; Institute of Biomedical Studies, Baylor University, South 5th Street, Waco, TX 76706, USA. FAU - Oh, SangKon AU - Oh S AD - Baylor Institute for Immunology Research, 3434 Live Oak Street, Dallas, TX 75204, USA; Institute of Biomedical Studies, Baylor University, South 5th Street, Waco, TX 76706, USA. LA - eng GR - U19 AI057234/AI/NIAID NIH HHS/United States PT - Journal Article PT - Research Support, N.I.H., Extramural PT - Research Support, Non-U.S. Gov't DEP - 20160128 PL - Netherlands TA - EBioMedicine JT - EBioMedicine JID - 101647039 RN - 0 (Lectins) RN - 0 (Lectins, C-Type) RN - 0 (Olr1 protein, mouse) RN - 0 (Recombinant Fusion Proteins) RN - 0 (Scavenger Receptors, Class E) RN - 0 (dectin 1) RN - 147205-72-9 (CD40 Ligand) SB - IM CIN - EBioMedicine. 2016 Mar;5:7-8. PMID: 27077097 MH - Animals MH - Antigen Presentation/*immunology MH - CD4-Positive T-Lymphocytes/immunology MH - CD40 Ligand/*immunology MH - CD8-Positive T-Lymphocytes/immunology MH - Cell Differentiation/immunology MH - Dendritic Cells/*immunology MH - Humans MH - *Immunotherapy, Active MH - Lectins/immunology MH - Lectins, C-Type/immunology MH - Lymphocyte Activation/*immunology MH - Mice MH - Mice, Transgenic MH - Recombinant Fusion Proteins/immunology MH - Scavenger Receptors, Class E/immunology PMC - PMC4816850 OTO - NOTNLM OT - ANOVA, analysis of variance OT - AP, alkaline phosphatase OT - APC, antigen-presenting cells OT - CD, cluster of differentiation OT - CD40 OT - CFSE, carboxyfluorescein succinimidyl ester OT - CTL, cytotoxic T lymphocyte OT - Coh, cohesin OT - Cross-presentation OT - DC, dendritic cell OT - Dendritic cell OT - Doc, dockerin OT - EEA1, early endosome antigen 1 OT - ELISA, enzyme-linked immunosorbent assay OT - ELISpot, enzyme-linked immunospot OT - Flu.M1, influenza virus matrix protein 1 OT - GM-CSF, granulocyte-macrophage colony-stimulating factor OT - HA1, hemagglutinin subunit 1 OT - HLA, human leukocyte antigen OT - HPV, human papillomavirus OT - HRP, horseradish peroxidase OT - IFN, interferon OT - IL, interleukin OT - JaCoP, Just another Colocalization Plugin OT - LAMP-1, lysosomal-associated membrane protein 1 OT - Lectins OT - MART-1, melanoma antigen recognized by T cells 1 OT - MHC, major histocompatibility complex OT - Mo-DC, monocyte-derived dendritic cell OT - NHP, non-human primate OT - NP, nucleoprotein OT - PBMC, peripheral blood mononuclear cells OT - PBS, phosphate-buffered saline OT - PSA, prostate specific antigen OT - Poly(I:C), polyinosinic:polycytidylic acid OT - TLR, toll-like receptor OT - TMB, 3,3',5,5'-tetramethylbenzidine OT - TNF, tumor necrosis factor OT - Vaccine OT - hCD40Tg, human CD40 transgenic OT - i.p., intraperitoneal(ly) OT - mAb, monoclonal antibody OT - mDC, myeloid dendritic cell OT - pDC, plasmacytoid dendritic cell OT - s.c., subcutaneous(ly) EDAT- 2016/04/15 06:00 MHDA- 2016/12/31 06:00 PMCR- 2016/01/28 CRDT- 2016/04/15 06:00 PHST- 2015/12/17 00:00 [received] PHST- 2016/01/21 00:00 [revised] PHST- 2016/01/25 00:00 [accepted] PHST- 2016/04/15 06:00 [entrez] PHST- 2016/04/15 06:00 [pubmed] PHST- 2016/12/31 06:00 [medline] PHST- 2016/01/28 00:00 [pmc-release] AID - S2352-3964(16)30026-3 [pii] AID - 10.1016/j.ebiom.2016.01.029 [doi] PST - epublish SO - EBioMedicine. 2016 Jan 28;5:46-58. doi: 10.1016/j.ebiom.2016.01.029. eCollection 2016 Mar.