PMID- 27077767
OWN - NLM
STAT- MEDLINE
DCOM- 20170405
LR  - 20220408
IS  - 1607-8454 (Electronic)
IS  - 1024-5332 (Linking)
VI  - 21
IP  - 7
DP  - 2016 Aug
TI  - Mad2 overexpression is associated with high cell proliferation and reduced 
      disease-free survival in primary gastrointestinal diffuse large B-cell lymphoma.
PG  - 399-403
LID - 10.1080/10245332.2015.1101970 [doi]
AB  - OBJECTIVES: Primary gastrointestinal diffuse large B-cell lymphoma (PGI-DLBCL) is 
      a rare hematological malignancy with limited results on carcinogenesis and 
      clinical characteristics. The aims of the current study were to examine mitotic 
      arrest deficiency protein 2 (Mad2) expressions in PGI-DLBCL, and assess its 
      association with Ki-67 expression, Helicobacter pylori (H. pylori) infection, 
      BCL-6 gene rearrangement, and clinicopathological variables. METHODS: Cancer 
      tissues from 38 PGI-DLBCL patients were examined for Mad2, Ki-67, and H. pylori 
      expression by immunohistochemistry, using normal gastrointestinal tissues and 
      nodal DLBCL as controls. BCL-6 gene translocation was analyzed by fluorescence in 
      situ hybridization (FISH), and Mad2 expression status was evaluated along with 
      clinicopathological characteristics. RESULTS: Mad2 expression was increased in 
      PGI-DLBCL patients when compared with controls. The expression of Mad2 was 
      51.55 +/- 22.88% in PGI-DLBCL, which was higher than reactive lymph node 
      (28.77 +/- 10.89%) and lymphoid nodule in normal gastrointestinal tissue 
      (26.41 +/- 11.30%) (P = 0.002), while it was comparable to nodal DLBCL 
      (57.23 +/- 20.79%) (P = 0.358). Mad2 overexpression had a positive correlation with 
      Ki-67 proliferation index (r = 0.55, P = 0.01) in PGI-DLBCL, and patients with 
      BCL-6 gene rearrangement had lower Mad2 expression (P = 0.032) than patients with 
      intact BCL-6, while no relation was found between Mad2 expression and H. pylori 
      infection. PGI-DLBCL patients with higher Mad2 expression had lower estimated 
      disease-free survival (DFS) (17.10% vs. 53.00%) (P = 0.049). However, no 
      correlation was found between Mad2 expression levels and overall survival (OS) 
      (P = 0.443). CONCLUSIONS: Aberrant Mad2 expression was associated with cell 
      proliferation and genetic instability, which may contribute to the carcinogenesis 
      of PGI-DLBCL. Mad2 overexpression indicated a poor DFS and may be a potential 
      biomarker for estimating prognosis for PGI-DLBCL patients.
FAU - Chen, Fei
AU  - Chen F
AD  - a Department of Hematology , Zhongnan Hospital of Wuhan University , 169 Dong Hu 
      Road, Wuhan 430071 , China.
FAU - Liu, Shangqin
AU  - Liu S
AD  - a Department of Hematology , Zhongnan Hospital of Wuhan University , 169 Dong Hu 
      Road, Wuhan 430071 , China.
FAU - Zhou, Yi
AU  - Zhou Y
AD  - a Department of Hematology , Zhongnan Hospital of Wuhan University , 169 Dong Hu 
      Road, Wuhan 430071 , China.
FAU - Shen, Hui
AU  - Shen H
AD  - a Department of Hematology , Zhongnan Hospital of Wuhan University , 169 Dong Hu 
      Road, Wuhan 430071 , China.
FAU - Zuo, Xuelan
AU  - Zuo X
AD  - a Department of Hematology , Zhongnan Hospital of Wuhan University , 169 Dong Hu 
      Road, Wuhan 430071 , China.
LA  - eng
PT  - Journal Article
DEP - 20160316
PL  - England
TA  - Hematology
JT  - Hematology (Amsterdam, Netherlands)
JID - 9708388
RN  - 0 (Ki-67 Antigen)
RN  - 0 (MAD2L1 protein, human)
RN  - 0 (Mad2 Proteins)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Case-Control Studies
MH  - Cell Proliferation/physiology
MH  - Disease-Free Survival
MH  - Female
MH  - Gastrointestinal Neoplasms/*metabolism/pathology
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Ki-67 Antigen/biosynthesis/metabolism
MH  - Lymphoma, Large B-Cell, Diffuse/genetics/*metabolism/pathology
MH  - Mad2 Proteins/*biosynthesis/genetics/metabolism
MH  - Male
MH  - Middle Aged
OTO - NOTNLM
OT  - B-cell lymphoma-6 gene (BCL-6)
OT  - Mitotic arrest deficiency protein 2 (Mad2)
OT  - Nuclear associated antigen (Ki-67)
OT  - Primary gastrointestinal diffuse large B-cell lymphoma (PGI-DLBCL)
EDAT- 2016/04/15 06:00
MHDA- 2017/04/06 06:00
CRDT- 2016/04/15 06:00
PHST- 2016/04/15 06:00 [entrez]
PHST- 2016/04/15 06:00 [pubmed]
PHST- 2017/04/06 06:00 [medline]
AID - 10.1080/10245332.2015.1101970 [doi]
PST - ppublish
SO  - Hematology. 2016 Aug;21(7):399-403. doi: 10.1080/10245332.2015.1101970. Epub 2016 
      Mar 16.